Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants

Abstract Genome stability in radioresistant bacterium Deinococcus radiodurans depends on RecA, the main bacterial recombinase. Without RecA, gross genome rearrangements occur during repair of DNA double-strand breaks. Long repeated (insertion) sequences have been identified as hot spots for ectopic...

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Autores principales:	Jelena Repar, Davor Zahradka, Ivan Sović, Ksenija Zahradka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85c9fef53f27432094950937f6ece77f
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spelling	oai:doaj.org-article:85c9fef53f27432094950937f6ece77f2021-12-02T15:49:45ZCharacterization of gross genome rearrangements in Deinococcus radiodurans recA mutants10.1038/s41598-021-89173-92045-2322https://doaj.org/article/85c9fef53f27432094950937f6ece77f2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89173-9https://doaj.org/toc/2045-2322Abstract Genome stability in radioresistant bacterium Deinococcus radiodurans depends on RecA, the main bacterial recombinase. Without RecA, gross genome rearrangements occur during repair of DNA double-strand breaks. Long repeated (insertion) sequences have been identified as hot spots for ectopic recombination leading to genome rearrangements, and single-strand annealing (SSA) postulated to be the most likely mechanism involved in this process. Here, we have sequenced five isolates of D. radiodurans recA mutant carrying gross genome rearrangements to precisely characterize the rearrangements and to elucidate the underlying repair mechanism. The detected rearrangements consisted of large deletions in chromosome II in all the sequenced recA isolates. The mechanism behind these deletions clearly differs from the classical SSA; it utilized short (4–11 bp) repeats as opposed to insertion sequences or other long repeats. Moreover, it worked over larger linear DNA distances from those previously tested. Our data are most compatible with alternative end-joining, a recombination mechanism that operates in eukaryotes, but is also found in Escherichia coli. Additionally, despite the recA isolates being preselected for different rearrangement patterns, all identified deletions were found to overlap in a 35 kb genomic region. We weigh the evidence for mechanistic vs. adaptive reasons for this phenomenon.Jelena ReparDavor ZahradkaIvan SovićKsenija ZahradkaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jelena Repar Davor Zahradka Ivan Sović Ksenija Zahradka Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants
description	Abstract Genome stability in radioresistant bacterium Deinococcus radiodurans depends on RecA, the main bacterial recombinase. Without RecA, gross genome rearrangements occur during repair of DNA double-strand breaks. Long repeated (insertion) sequences have been identified as hot spots for ectopic recombination leading to genome rearrangements, and single-strand annealing (SSA) postulated to be the most likely mechanism involved in this process. Here, we have sequenced five isolates of D. radiodurans recA mutant carrying gross genome rearrangements to precisely characterize the rearrangements and to elucidate the underlying repair mechanism. The detected rearrangements consisted of large deletions in chromosome II in all the sequenced recA isolates. The mechanism behind these deletions clearly differs from the classical SSA; it utilized short (4–11 bp) repeats as opposed to insertion sequences or other long repeats. Moreover, it worked over larger linear DNA distances from those previously tested. Our data are most compatible with alternative end-joining, a recombination mechanism that operates in eukaryotes, but is also found in Escherichia coli. Additionally, despite the recA isolates being preselected for different rearrangement patterns, all identified deletions were found to overlap in a 35 kb genomic region. We weigh the evidence for mechanistic vs. adaptive reasons for this phenomenon.
format	article
author	Jelena Repar Davor Zahradka Ivan Sović Ksenija Zahradka
author_facet	Jelena Repar Davor Zahradka Ivan Sović Ksenija Zahradka
author_sort	Jelena Repar
title	Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants
title_short	Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants
title_full	Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants
title_fullStr	Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants
title_full_unstemmed	Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants
title_sort	characterization of gross genome rearrangements in deinococcus radiodurans reca mutants
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/85c9fef53f27432094950937f6ece77f
work_keys_str_mv	AT jelenarepar characterizationofgrossgenomerearrangementsindeinococcusradioduransrecamutants AT davorzahradka characterizationofgrossgenomerearrangementsindeinococcusradioduransrecamutants AT ivansovic characterizationofgrossgenomerearrangementsindeinococcusradioduransrecamutants AT ksenijazahradka characterizationofgrossgenomerearrangementsindeinococcusradioduransrecamutants
_version_	1718385702365822976

Characterization of gross genome rearrangements in Deinococcus radiodurans recA mutants

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