Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.

Cadherins, Ca(2+)-dependent adhesion molecules, are crucial for cell-cell junctions and remodeling. Cadherins form inter-junctional lattices by the formation of both cis and trans dimers. Here, we directly visualize and quantify the spatiotemporal dynamics of wild-type and dimer mutant N-cadherin in...

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Autores principales: Stefanie Bunse, Sakshi Garg, Stephan Junek, Dirk Vogel, Nariman Ansari, Ernst H K Stelzer, Erin Schuman
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Publicado: Public Library of Science (PLoS) 2013
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Acceso en línea:https://doaj.org/article/85d759e24ede4870952c6b82b5a313d5
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spelling oai:doaj.org-article:85d759e24ede4870952c6b82b5a313d52021-11-18T08:43:57ZRole of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.1932-620310.1371/journal.pone.0081517https://doaj.org/article/85d759e24ede4870952c6b82b5a313d52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312555/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cadherins, Ca(2+)-dependent adhesion molecules, are crucial for cell-cell junctions and remodeling. Cadherins form inter-junctional lattices by the formation of both cis and trans dimers. Here, we directly visualize and quantify the spatiotemporal dynamics of wild-type and dimer mutant N-cadherin interactions using time-lapse imaging of junction assembly, disassembly and a FRET reporter to assess Ca(2+)-dependent interactions. A trans dimer mutant (W2A) and a cis mutant (V81D/V174D) exhibited an increased Ca(2+)-sensitivity for the disassembly of trans dimers compared to the WT, while another mutant (R14E) was insensitive to Ca(2+)-chelation. Time-lapse imaging of junction assembly and disassembly, monitored in 2D and 3D (using cellular spheroids), revealed kinetic differences in the different mutants as well as different behaviors in the 2D and 3D environment. Taken together, these data provide new insights into the role that the cis and trans dimers play in the dynamic interactions of cadherins.Stefanie BunseSakshi GargStephan JunekDirk VogelNariman AnsariErnst H K StelzerErin SchumanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81517 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Stefanie Bunse
Sakshi Garg
Stephan Junek
Dirk Vogel
Nariman Ansari
Ernst H K Stelzer
Erin Schuman
Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.
description Cadherins, Ca(2+)-dependent adhesion molecules, are crucial for cell-cell junctions and remodeling. Cadherins form inter-junctional lattices by the formation of both cis and trans dimers. Here, we directly visualize and quantify the spatiotemporal dynamics of wild-type and dimer mutant N-cadherin interactions using time-lapse imaging of junction assembly, disassembly and a FRET reporter to assess Ca(2+)-dependent interactions. A trans dimer mutant (W2A) and a cis mutant (V81D/V174D) exhibited an increased Ca(2+)-sensitivity for the disassembly of trans dimers compared to the WT, while another mutant (R14E) was insensitive to Ca(2+)-chelation. Time-lapse imaging of junction assembly and disassembly, monitored in 2D and 3D (using cellular spheroids), revealed kinetic differences in the different mutants as well as different behaviors in the 2D and 3D environment. Taken together, these data provide new insights into the role that the cis and trans dimers play in the dynamic interactions of cadherins.
format article
author Stefanie Bunse
Sakshi Garg
Stephan Junek
Dirk Vogel
Nariman Ansari
Ernst H K Stelzer
Erin Schuman
author_facet Stefanie Bunse
Sakshi Garg
Stephan Junek
Dirk Vogel
Nariman Ansari
Ernst H K Stelzer
Erin Schuman
author_sort Stefanie Bunse
title Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.
title_short Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.
title_full Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.
title_fullStr Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.
title_full_unstemmed Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.
title_sort role of n-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/85d759e24ede4870952c6b82b5a313d5
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AT sakshigarg roleofncadherincisandtransinterfacesinthedynamicsofadherensjunctionsinlivingcells
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