Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.
Cadherins, Ca(2+)-dependent adhesion molecules, are crucial for cell-cell junctions and remodeling. Cadherins form inter-junctional lattices by the formation of both cis and trans dimers. Here, we directly visualize and quantify the spatiotemporal dynamics of wild-type and dimer mutant N-cadherin in...
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2013
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oai:doaj.org-article:85d759e24ede4870952c6b82b5a313d52021-11-18T08:43:57ZRole of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells.1932-620310.1371/journal.pone.0081517https://doaj.org/article/85d759e24ede4870952c6b82b5a313d52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312555/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cadherins, Ca(2+)-dependent adhesion molecules, are crucial for cell-cell junctions and remodeling. Cadherins form inter-junctional lattices by the formation of both cis and trans dimers. Here, we directly visualize and quantify the spatiotemporal dynamics of wild-type and dimer mutant N-cadherin interactions using time-lapse imaging of junction assembly, disassembly and a FRET reporter to assess Ca(2+)-dependent interactions. A trans dimer mutant (W2A) and a cis mutant (V81D/V174D) exhibited an increased Ca(2+)-sensitivity for the disassembly of trans dimers compared to the WT, while another mutant (R14E) was insensitive to Ca(2+)-chelation. Time-lapse imaging of junction assembly and disassembly, monitored in 2D and 3D (using cellular spheroids), revealed kinetic differences in the different mutants as well as different behaviors in the 2D and 3D environment. Taken together, these data provide new insights into the role that the cis and trans dimers play in the dynamic interactions of cadherins.Stefanie BunseSakshi GargStephan JunekDirk VogelNariman AnsariErnst H K StelzerErin SchumanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81517 (2013) |
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Medicine R Science Q Stefanie Bunse Sakshi Garg Stephan Junek Dirk Vogel Nariman Ansari Ernst H K Stelzer Erin Schuman Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells. |
description |
Cadherins, Ca(2+)-dependent adhesion molecules, are crucial for cell-cell junctions and remodeling. Cadherins form inter-junctional lattices by the formation of both cis and trans dimers. Here, we directly visualize and quantify the spatiotemporal dynamics of wild-type and dimer mutant N-cadherin interactions using time-lapse imaging of junction assembly, disassembly and a FRET reporter to assess Ca(2+)-dependent interactions. A trans dimer mutant (W2A) and a cis mutant (V81D/V174D) exhibited an increased Ca(2+)-sensitivity for the disassembly of trans dimers compared to the WT, while another mutant (R14E) was insensitive to Ca(2+)-chelation. Time-lapse imaging of junction assembly and disassembly, monitored in 2D and 3D (using cellular spheroids), revealed kinetic differences in the different mutants as well as different behaviors in the 2D and 3D environment. Taken together, these data provide new insights into the role that the cis and trans dimers play in the dynamic interactions of cadherins. |
format |
article |
author |
Stefanie Bunse Sakshi Garg Stephan Junek Dirk Vogel Nariman Ansari Ernst H K Stelzer Erin Schuman |
author_facet |
Stefanie Bunse Sakshi Garg Stephan Junek Dirk Vogel Nariman Ansari Ernst H K Stelzer Erin Schuman |
author_sort |
Stefanie Bunse |
title |
Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells. |
title_short |
Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells. |
title_full |
Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells. |
title_fullStr |
Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells. |
title_full_unstemmed |
Role of N-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells. |
title_sort |
role of n-cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doaj.org/article/85d759e24ede4870952c6b82b5a313d5 |
work_keys_str_mv |
AT stefaniebunse roleofncadherincisandtransinterfacesinthedynamicsofadherensjunctionsinlivingcells AT sakshigarg roleofncadherincisandtransinterfacesinthedynamicsofadherensjunctionsinlivingcells AT stephanjunek roleofncadherincisandtransinterfacesinthedynamicsofadherensjunctionsinlivingcells AT dirkvogel roleofncadherincisandtransinterfacesinthedynamicsofadherensjunctionsinlivingcells AT narimanansari roleofncadherincisandtransinterfacesinthedynamicsofadherensjunctionsinlivingcells AT ernsthkstelzer roleofncadherincisandtransinterfacesinthedynamicsofadherensjunctionsinlivingcells AT erinschuman roleofncadherincisandtransinterfacesinthedynamicsofadherensjunctionsinlivingcells |
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1718421392253255680 |