Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis

Fifty-two Staphylococcus aureus recovered from papillary ostium and milk samples collected from cows with subclinical mastitis and milking environments in three small dairy herds located in southeastern Brazil were subjected to PCR identification based on the thermonuclease (nuc) gene. All the stra...

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Autores principales:	Felipe Mendes Zorzi, Luiz Francisco Zafalon, Franklin Bispo Santos, Arthur Ferreira Borges, Ticiano Gomes Nascimento, Irinaldo Diniz Basílio-Júnior, Elsa Masae Mamizuka, Lara Mendes Almeida
Formato:	article
Lenguaje:	EN PT
Publicado:	Universidade de São Paulo 2021
Materias:	MSSA MIONSA Accessory gene regulator (agr) Bovine mastitis Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/85decd9e07974f2ea6b31f03addf2015
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spelling	oai:doaj.org-article:85decd9e07974f2ea6b31f03addf20152021-12-01T16:01:51ZVirulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis1413-95961678-4456https://doaj.org/article/85decd9e07974f2ea6b31f03addf20152021-12-01T00:00:00Zhttps://www.revistas.usp.br/bjvras/article/view/186701https://doaj.org/toc/1413-9596https://doaj.org/toc/1678-4456 Fifty-two Staphylococcus aureus recovered from papillary ostium and milk samples collected from cows with subclinical mastitis and milking environments in three small dairy herds located in southeastern Brazil were subjected to PCR identification based on the thermonuclease (nuc) gene. All the strains were submitted to in vitro antimicrobial susceptibility testing, and we investigated the sequence types (STs), agr groups (I-IV), virulence genes encoding for Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs), biofilm-associated proteins, bi-component toxins, pyrogenic toxin superantigens, and enterotoxins. Screening for oxacillin resistance (2-6 μg/ml oxacillin), beta-lactamase activity assays, and PCR for the mecA/mecC genes detected 26 methicillin-susceptible S. aureus (MSSA) and 26 mec-independent oxacillin-nonsusceptible S. aureus (MIONSA). While MSSA isolates were found to be susceptible to all antimicrobial agents tested, or only resistant to penicillin and ampicillin, MIONSA isolates were multidrug-resistant. ST126-agr group II MSSA isolates were prevalent in milk (n=14) and carried a broad set of virulence genes (clfA, clfB, eno, fnbA, fiB, icaA, icaD, lukED, hla, and hlb), as well as the ST126-agr group II MIONSA isolated from milking liners (n=1), which also carried the eta gene. ST1-agr group III MIONSA isolates (n=4) were found in papillary ostium and milk, but most MIONSA isolates (n=21), which were identified in both papillary ostium and milking liners, were agr-negative and assigned to ST126. The agr-negative and agr group III lineages showed a low potential for virulence. Studies on the characterization of bovine-associated MSSA/MIONSA are essential to reduce S. aureus mastitis to prevent economic losses in dairy production and also to monitor the zoonotic potential of these pathogens associated with invasive infections and treatment failures in healthcare. Felipe Mendes ZorziLuiz Francisco ZafalonFranklin Bispo SantosArthur Ferreira BorgesTiciano Gomes NascimentoIrinaldo Diniz Basílio-JúniorElsa Masae MamizukaLara Mendes AlmeidaUniversidade de São PauloarticleMSSAMIONSAAccessory gene regulator (agr)Bovine mastitisAnimal cultureSF1-1100ENPTBrazilian Journal of Veterinary Research and Animal Science, Vol 58 (2021)
institution	DOAJ
collection	DOAJ
language	EN PT
topic	MSSA MIONSA Accessory gene regulator (agr) Bovine mastitis Animal culture SF1-1100
spellingShingle	MSSA MIONSA Accessory gene regulator (agr) Bovine mastitis Animal culture SF1-1100 Felipe Mendes Zorzi Luiz Francisco Zafalon Franklin Bispo Santos Arthur Ferreira Borges Ticiano Gomes Nascimento Irinaldo Diniz Basílio-Júnior Elsa Masae Mamizuka Lara Mendes Almeida Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis
description	Fifty-two Staphylococcus aureus recovered from papillary ostium and milk samples collected from cows with subclinical mastitis and milking environments in three small dairy herds located in southeastern Brazil were subjected to PCR identification based on the thermonuclease (nuc) gene. All the strains were submitted to in vitro antimicrobial susceptibility testing, and we investigated the sequence types (STs), agr groups (I-IV), virulence genes encoding for Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs), biofilm-associated proteins, bi-component toxins, pyrogenic toxin superantigens, and enterotoxins. Screening for oxacillin resistance (2-6 μg/ml oxacillin), beta-lactamase activity assays, and PCR for the mecA/mecC genes detected 26 methicillin-susceptible S. aureus (MSSA) and 26 mec-independent oxacillin-nonsusceptible S. aureus (MIONSA). While MSSA isolates were found to be susceptible to all antimicrobial agents tested, or only resistant to penicillin and ampicillin, MIONSA isolates were multidrug-resistant. ST126-agr group II MSSA isolates were prevalent in milk (n=14) and carried a broad set of virulence genes (clfA, clfB, eno, fnbA, fiB, icaA, icaD, lukED, hla, and hlb), as well as the ST126-agr group II MIONSA isolated from milking liners (n=1), which also carried the eta gene. ST1-agr group III MIONSA isolates (n=4) were found in papillary ostium and milk, but most MIONSA isolates (n=21), which were identified in both papillary ostium and milking liners, were agr-negative and assigned to ST126. The agr-negative and agr group III lineages showed a low potential for virulence. Studies on the characterization of bovine-associated MSSA/MIONSA are essential to reduce S. aureus mastitis to prevent economic losses in dairy production and also to monitor the zoonotic potential of these pathogens associated with invasive infections and treatment failures in healthcare.
format	article
author	Felipe Mendes Zorzi Luiz Francisco Zafalon Franklin Bispo Santos Arthur Ferreira Borges Ticiano Gomes Nascimento Irinaldo Diniz Basílio-Júnior Elsa Masae Mamizuka Lara Mendes Almeida
author_facet	Felipe Mendes Zorzi Luiz Francisco Zafalon Franklin Bispo Santos Arthur Ferreira Borges Ticiano Gomes Nascimento Irinaldo Diniz Basílio-Júnior Elsa Masae Mamizuka Lara Mendes Almeida
author_sort	Felipe Mendes Zorzi
title	Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis
title_short	Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis
title_full	Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis
title_fullStr	Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis
title_full_unstemmed	Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis
title_sort	virulence, agr groups, antimicrobial resistance and epidemiology of staphylococcus aureus isolated from bovine subclinical mastitis
publisher	Universidade de São Paulo
publishDate	2021
url	https://doaj.org/article/85decd9e07974f2ea6b31f03addf2015
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Virulence, agr groups, antimicrobial resistance and epidemiology of Staphylococcus aureus isolated from bovine subclinical mastitis

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