The olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.

The olfactory bulb (OB) periventricular zone is an extension of the forebrain subventricular zone (SVZ) and thus is a source of neuroprogenitor cells and neural stem cells. While considerable information is available on the SVZ-OB neural stem cell (NSC)/neuroprogenitor cell (NPC) niche in rodents, l...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lee J Martin, Alyssa Katzenelson, Raymond C Koehler, Qing Chang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85e22a6ffb164e288ae32b9e1c32b2c6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:85e22a6ffb164e288ae32b9e1c32b2c6
record_format	dspace
spelling	oai:doaj.org-article:85e22a6ffb164e288ae32b9e1c32b2c62021-11-18T08:45:10ZThe olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.1932-620310.1371/journal.pone.0081105https://doaj.org/article/85e22a6ffb164e288ae32b9e1c32b2c62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278384/?tool=EBIhttps://doaj.org/toc/1932-6203The olfactory bulb (OB) periventricular zone is an extension of the forebrain subventricular zone (SVZ) and thus is a source of neuroprogenitor cells and neural stem cells. While considerable information is available on the SVZ-OB neural stem cell (NSC)/neuroprogenitor cell (NPC) niche in rodents, less work has been done on this system in large animals. The newborn piglet is used as a preclinical translational model of neonatal hypoxic-ischemic brain damage, but information about the endogenous sources of NSCs/NPCs in piglet is needed to implement endogenous or autologous cell-based therapies in this model. We characterized NSC/NPC niches in piglet forebrain and OB-SVZ using western blotting, histological, and cell culture methods. Immunoblotting revealed nestin, a NSC/NPC marker, in forebrain-SVZ and OB-SVZ in newborn piglet. Several progenitor or newborn neuron markers, including Dlx2, musashi, doublecortin, and polysialated neural cell adhesion molecule were also detected in OB-SVZ by immunoblotting. Immunohistochemistry confirmed the presence of nestin, musashi, and doublecortin in forebrain-SVZ and OB-SVZ. Bromodeoxyuridine (BrdU) labeling showed that the forebrain-SVZ and OB-SVZ accumulate newly replicated cells. BrdU-positive cells were immunolabeled for astroglial, oligodendroglial, and neuronal markers. A lateral migratory pathway for newly born neuron migration to primary olfactory cortex was revealed by BrdU labeling and co-labeling for doublecortin and class III β tubulin. Isolated and cultured forebrain-SVZ and OB-SVZ cells from newborn piglet had the capacity to generate numerous neurospheres. Single cell clonal analysis of neurospheres revealed the capacity for self-renewal and multipotency. Neurosphere-derived cells differentiated into neurons, astrocytes, and oligodendrocytes and were amenable to permanent genetic tagging with lentivirus encoding green fluorescent protein. We conclude that the piglet OB-SVZ is a reservoir of NSCs and NPCs suitable to use in autologous cell therapy in preclinical models of neonatal/pediatric brain injury.Lee J MartinAlyssa KatzenelsonRaymond C KoehlerQing ChangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e81105 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lee J Martin Alyssa Katzenelson Raymond C Koehler Qing Chang The olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.
description	The olfactory bulb (OB) periventricular zone is an extension of the forebrain subventricular zone (SVZ) and thus is a source of neuroprogenitor cells and neural stem cells. While considerable information is available on the SVZ-OB neural stem cell (NSC)/neuroprogenitor cell (NPC) niche in rodents, less work has been done on this system in large animals. The newborn piglet is used as a preclinical translational model of neonatal hypoxic-ischemic brain damage, but information about the endogenous sources of NSCs/NPCs in piglet is needed to implement endogenous or autologous cell-based therapies in this model. We characterized NSC/NPC niches in piglet forebrain and OB-SVZ using western blotting, histological, and cell culture methods. Immunoblotting revealed nestin, a NSC/NPC marker, in forebrain-SVZ and OB-SVZ in newborn piglet. Several progenitor or newborn neuron markers, including Dlx2, musashi, doublecortin, and polysialated neural cell adhesion molecule were also detected in OB-SVZ by immunoblotting. Immunohistochemistry confirmed the presence of nestin, musashi, and doublecortin in forebrain-SVZ and OB-SVZ. Bromodeoxyuridine (BrdU) labeling showed that the forebrain-SVZ and OB-SVZ accumulate newly replicated cells. BrdU-positive cells were immunolabeled for astroglial, oligodendroglial, and neuronal markers. A lateral migratory pathway for newly born neuron migration to primary olfactory cortex was revealed by BrdU labeling and co-labeling for doublecortin and class III β tubulin. Isolated and cultured forebrain-SVZ and OB-SVZ cells from newborn piglet had the capacity to generate numerous neurospheres. Single cell clonal analysis of neurospheres revealed the capacity for self-renewal and multipotency. Neurosphere-derived cells differentiated into neurons, astrocytes, and oligodendrocytes and were amenable to permanent genetic tagging with lentivirus encoding green fluorescent protein. We conclude that the piglet OB-SVZ is a reservoir of NSCs and NPCs suitable to use in autologous cell therapy in preclinical models of neonatal/pediatric brain injury.
format	article
author	Lee J Martin Alyssa Katzenelson Raymond C Koehler Qing Chang
author_facet	Lee J Martin Alyssa Katzenelson Raymond C Koehler Qing Chang
author_sort	Lee J Martin
title	The olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.
title_short	The olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.
title_full	The olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.
title_fullStr	The olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.
title_full_unstemmed	The olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.
title_sort	olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/85e22a6ffb164e288ae32b9e1c32b2c6
work_keys_str_mv	AT leejmartin theolfactorybulbinnewbornpigletisareservoirofneuralstemandprogenitorcells AT alyssakatzenelson theolfactorybulbinnewbornpigletisareservoirofneuralstemandprogenitorcells AT raymondckoehler theolfactorybulbinnewbornpigletisareservoirofneuralstemandprogenitorcells AT qingchang theolfactorybulbinnewbornpigletisareservoirofneuralstemandprogenitorcells AT leejmartin olfactorybulbinnewbornpigletisareservoirofneuralstemandprogenitorcells AT alyssakatzenelson olfactorybulbinnewbornpigletisareservoirofneuralstemandprogenitorcells AT raymondckoehler olfactorybulbinnewbornpigletisareservoirofneuralstemandprogenitorcells AT qingchang olfactorybulbinnewbornpigletisareservoirofneuralstemandprogenitorcells
_version_	1718421392456679424

The olfactory bulb in newborn piglet is a reservoir of neural stem and progenitor cells.

Ejemplares similares