The virome of German bats: comparing virus discovery approaches

Abstract Bats are known to be reservoirs of several highly pathogenic viruses. Hence, the interest in bat virus discovery has been increasing rapidly over the last decade. So far, most studies have focused on a single type of virus detection method, either PCR, virus isolation or virome sequencing....

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Autores principales:	Claudia Kohl, Annika Brinkmann, Aleksandar Radonić, Piotr Wojtek Dabrowski, Kristin Mühldorfer, Andreas Nitsche, Gudrun Wibbelt, Andreas Kurth
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85e68398e9e64266b967e1adbcd3f176
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spelling	oai:doaj.org-article:85e68398e9e64266b967e1adbcd3f1762021-12-02T14:25:27ZThe virome of German bats: comparing virus discovery approaches10.1038/s41598-021-86435-42045-2322https://doaj.org/article/85e68398e9e64266b967e1adbcd3f1762021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86435-4https://doaj.org/toc/2045-2322Abstract Bats are known to be reservoirs of several highly pathogenic viruses. Hence, the interest in bat virus discovery has been increasing rapidly over the last decade. So far, most studies have focused on a single type of virus detection method, either PCR, virus isolation or virome sequencing. Here we present a comprehensive approach in virus discovery, using all three discovery methods on samples from the same bats. By family-specific PCR screening we found sequences of paramyxoviruses, adenoviruses, herpesviruses and one coronavirus. By cell culture we isolated a novel bat adenovirus and bat orthoreovirus. Virome sequencing revealed viral sequences of ten different virus families and orders: three bat nairoviruses, three phenuiviruses, one orbivirus, one rotavirus, one orthoreovirus, one mononegavirus, five parvoviruses, seven picornaviruses, three retroviruses, one totivirus and two thymoviruses were discovered. Of all viruses identified by family-specific PCR in the original samples, none was found by metagenomic sequencing. Vice versa, none of the viruses found by the metagenomic virome approach was detected by family-specific PCRs targeting the same family. The discrepancy of detected viruses by different detection approaches suggests that a combined approach using different detection methods is necessary for virus discovery studies.Claudia KohlAnnika BrinkmannAleksandar RadonićPiotr Wojtek DabrowskiKristin MühldorferAndreas NitscheGudrun WibbeltAndreas KurthNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claudia Kohl Annika Brinkmann Aleksandar Radonić Piotr Wojtek Dabrowski Kristin Mühldorfer Andreas Nitsche Gudrun Wibbelt Andreas Kurth The virome of German bats: comparing virus discovery approaches
description	Abstract Bats are known to be reservoirs of several highly pathogenic viruses. Hence, the interest in bat virus discovery has been increasing rapidly over the last decade. So far, most studies have focused on a single type of virus detection method, either PCR, virus isolation or virome sequencing. Here we present a comprehensive approach in virus discovery, using all three discovery methods on samples from the same bats. By family-specific PCR screening we found sequences of paramyxoviruses, adenoviruses, herpesviruses and one coronavirus. By cell culture we isolated a novel bat adenovirus and bat orthoreovirus. Virome sequencing revealed viral sequences of ten different virus families and orders: three bat nairoviruses, three phenuiviruses, one orbivirus, one rotavirus, one orthoreovirus, one mononegavirus, five parvoviruses, seven picornaviruses, three retroviruses, one totivirus and two thymoviruses were discovered. Of all viruses identified by family-specific PCR in the original samples, none was found by metagenomic sequencing. Vice versa, none of the viruses found by the metagenomic virome approach was detected by family-specific PCRs targeting the same family. The discrepancy of detected viruses by different detection approaches suggests that a combined approach using different detection methods is necessary for virus discovery studies.
format	article
author	Claudia Kohl Annika Brinkmann Aleksandar Radonić Piotr Wojtek Dabrowski Kristin Mühldorfer Andreas Nitsche Gudrun Wibbelt Andreas Kurth
author_facet	Claudia Kohl Annika Brinkmann Aleksandar Radonić Piotr Wojtek Dabrowski Kristin Mühldorfer Andreas Nitsche Gudrun Wibbelt Andreas Kurth
author_sort	Claudia Kohl
title	The virome of German bats: comparing virus discovery approaches
title_short	The virome of German bats: comparing virus discovery approaches
title_full	The virome of German bats: comparing virus discovery approaches
title_fullStr	The virome of German bats: comparing virus discovery approaches
title_full_unstemmed	The virome of German bats: comparing virus discovery approaches
title_sort	virome of german bats: comparing virus discovery approaches
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/85e68398e9e64266b967e1adbcd3f176
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The virome of German bats: comparing virus discovery approaches

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