Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles

Catherine A Schütz,1,* Davide Staedler,2,* Kieran Crosbie-Staunton,3 Dania Movia,4 Catherine Chapuis Bernasconi,1 Blanka Halamoda Kenzaoui,1 Adriele Prina-Mello,3,4 Lucienne Juillerat-Jeanneret11Centre Hospitalier Universitaire Vaudois (CHUV), UNIL, 2Institute of Chemical Sciences and Engi...

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Autores principales:	Schütz CA, Staedler D, Crosbie-Staunton K, Movia D, Chapuis Bernasconi C, Kenzaoui BH, Prina-Mello A, Juillerat-Jeanneret L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/85f1266d34644614b5a4cf6bef5e2bb7
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spelling	oai:doaj.org-article:85f1266d34644614b5a4cf6bef5e2bb72021-12-02T00:39:10ZDifferential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles1178-2013https://doaj.org/article/85f1266d34644614b5a4cf6bef5e2bb72014-07-01T00:00:00Zhttp://www.dovepress.com/differential-stress-reaction-of-human-colon-cells-to-oleic-acid-stabil-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Catherine A Schütz,1,* Davide Staedler,2,* Kieran Crosbie-Staunton,3 Dania Movia,4 Catherine Chapuis Bernasconi,1 Blanka Halamoda Kenzaoui,1 Adriele Prina-Mello,3,4 Lucienne Juillerat-Jeanneret11Centre Hospitalier Universitaire Vaudois (CHUV), UNIL, 2Institute of Chemical Sciences and Engineering, EPFL, CH-1015, Lausanne, Switzerland; 3School of Medicine, 4CRANN, Trinity College Dublin, Dublin, Ireland*These authors contributed equally to this workAbstract: Therapeutic engineered nanoparticles (NPs), including ultrasmall superparamagnetic iron oxide (USPIO) NPs, may accumulate in the lower digestive tract following ingestion or injection. In order to evaluate the reaction of human colon cells to USPIO NPs, the effects of non-stabilized USPIO NPs (NS-USPIO NPs), oleic-acid-stabilized USPIO NPs (OA-USPIO NPs), and free oleic acid (OA) were compared in human HT29 and CaCo2 colon epithelial cancer cells. First the biophysical characteristics of NS-USPIO NPs and OA-USPIO NPs in water, in cell culture medium supplemented with fetal calf serum, and in cell culture medium preconditioned by HT29 and CaCo2 cells were determined. Then, stress responses of the cells were evaluated following exposure to NS-USPIO NPs, OA-USPIO NPs, and free OA. No modification of the cytoskeletal actin network was observed. Cell response to stress, including markers of apoptosis and DNA repair, oxidative stress and degradative/autophagic stress, induction of heat shock protein, or lipid metabolism was determined in cells exposed to the two NPs. Induction of an autophagic response was observed in the two cell lines for both NPs but not free OA, while the other stress responses were cell- and NP-specific. The formation of lipid vacuoles/droplets was demonstrated in HT29 and CaCo2 cells exposed to OA-USPIO NPs but not to NS-USPIO NPs, and to a much lower level in cells exposed to equimolar concentrations of free OA. Therefore, the induction of lipid vacuoles in colon cells exposed to OA utilized as a stabilizer for USPIO NPs is higly amplified compared to free OA, and is not observed in the absence of this lipid in NS-USPIO NPs.Keywords: oleic acid, ultrasmall iron oxide nanoparticles, human colon cells, lipid vacuoles, stress reaction, heat shock proteinsSchütz CAStaedler DCrosbie-Staunton KMovia DChapuis Bernasconi CKenzaoui BHPrina-Mello AJuillerat-Jeanneret LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3481-3498 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Schütz CA Staedler D Crosbie-Staunton K Movia D Chapuis Bernasconi C Kenzaoui BH Prina-Mello A Juillerat-Jeanneret L Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles
description	Catherine A Schütz,1,* Davide Staedler,2,* Kieran Crosbie-Staunton,3 Dania Movia,4 Catherine Chapuis Bernasconi,1 Blanka Halamoda Kenzaoui,1 Adriele Prina-Mello,3,4 Lucienne Juillerat-Jeanneret11Centre Hospitalier Universitaire Vaudois (CHUV), UNIL, 2Institute of Chemical Sciences and Engineering, EPFL, CH-1015, Lausanne, Switzerland; 3School of Medicine, 4CRANN, Trinity College Dublin, Dublin, Ireland*These authors contributed equally to this workAbstract: Therapeutic engineered nanoparticles (NPs), including ultrasmall superparamagnetic iron oxide (USPIO) NPs, may accumulate in the lower digestive tract following ingestion or injection. In order to evaluate the reaction of human colon cells to USPIO NPs, the effects of non-stabilized USPIO NPs (NS-USPIO NPs), oleic-acid-stabilized USPIO NPs (OA-USPIO NPs), and free oleic acid (OA) were compared in human HT29 and CaCo2 colon epithelial cancer cells. First the biophysical characteristics of NS-USPIO NPs and OA-USPIO NPs in water, in cell culture medium supplemented with fetal calf serum, and in cell culture medium preconditioned by HT29 and CaCo2 cells were determined. Then, stress responses of the cells were evaluated following exposure to NS-USPIO NPs, OA-USPIO NPs, and free OA. No modification of the cytoskeletal actin network was observed. Cell response to stress, including markers of apoptosis and DNA repair, oxidative stress and degradative/autophagic stress, induction of heat shock protein, or lipid metabolism was determined in cells exposed to the two NPs. Induction of an autophagic response was observed in the two cell lines for both NPs but not free OA, while the other stress responses were cell- and NP-specific. The formation of lipid vacuoles/droplets was demonstrated in HT29 and CaCo2 cells exposed to OA-USPIO NPs but not to NS-USPIO NPs, and to a much lower level in cells exposed to equimolar concentrations of free OA. Therefore, the induction of lipid vacuoles in colon cells exposed to OA utilized as a stabilizer for USPIO NPs is higly amplified compared to free OA, and is not observed in the absence of this lipid in NS-USPIO NPs.Keywords: oleic acid, ultrasmall iron oxide nanoparticles, human colon cells, lipid vacuoles, stress reaction, heat shock proteins
format	article
author	Schütz CA Staedler D Crosbie-Staunton K Movia D Chapuis Bernasconi C Kenzaoui BH Prina-Mello A Juillerat-Jeanneret L
author_facet	Schütz CA Staedler D Crosbie-Staunton K Movia D Chapuis Bernasconi C Kenzaoui BH Prina-Mello A Juillerat-Jeanneret L
author_sort	Schütz CA
title	Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles
title_short	Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles
title_full	Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles
title_fullStr	Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles
title_full_unstemmed	Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles
title_sort	differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/85f1266d34644614b5a4cf6bef5e2bb7
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Differential stress reaction of human colon cells to oleic-acid-stabilized and unstabilized ultrasmall iron oxide nanoparticles

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