Detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR

Objective: The aim of this study was to determine the etiology and prevalence of pediatric CAP in Beijing using a real-time polymerase chain reaction (PCR) technique. Methods: Between February 15, 2011 and January 18, 2012, 371 pediatric patients with CAP were enrolled at Beijing Children's Hos...

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Autores principales:	Tie-Gang Zhang, Ai-Hua Li, Min Lyu, Meng Chen, Fang Huang, Jiang Wu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/85f215a60b614c1a8c9d2bbde41cf76e
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spelling	oai:doaj.org-article:85f215a60b614c1a8c9d2bbde41cf76e2021-12-02T15:51:49ZDetection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR2095-882X10.1016/j.cdtm.2015.06.002https://doaj.org/article/85f215a60b614c1a8c9d2bbde41cf76e2015-06-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2095882X1500033Xhttps://doaj.org/toc/2095-882XObjective: The aim of this study was to determine the etiology and prevalence of pediatric CAP in Beijing using a real-time polymerase chain reaction (PCR) technique. Methods: Between February 15, 2011 and January 18, 2012, 371 pediatric patients with CAP were enrolled at Beijing Children's Hospital. Sixteen respiratory viruses and two bacteria were detected from tracheal aspirate specimens using commercially available multiplex real-time reverse transcription PCR (RT-PCR) kits. Results: A single viral pathogen was detected in 35.3% of enrolled patients, multiple viruses in 11.6%, and virus/bacteria coinfection in 17.8%. In contrast, only 6.5% of patients had a single bacterial pathogen and 2.2% were infected with multiple bacteria. The etiological agent was unknown for 26.7% of patients. The most common viruses were respiratory syncytial virus (RSV) (43.9%), rhinovirus (14.8%), parainï¬uenza virus (9.4%), and adenovirus (8.6%). In patients under three years of age, RSV (44.6%), rhinovirus (12.8%), and Streptococcus pneumoniae (9.9%) were the most frequent pathogens. In children aged 3â7 years, S. pneumoniae (38.9%), RSV (30.6%), Haemophilus inï¬uenzae (19.4%), and adenovirus (19.4%) were most prevalent. Finally in children over seven years, RSV (47.3%), S. pneumoniae (41.9%), and rhinovirus (21.5%) infections were most frequent. Conclusions: Viral pathogens, specifically RSV, were responsible for the majority of CAP in pediatric patients. However, both S. pneumoniae and H. inï¬uenzae contributed as major causes of disease. Commercially available multiplexing real-time PCR allowed for rapid detection of the etiological agent. Keywords: Real-time reverse transcription polymerase chain reaction (RT-PCR), Respiratory virus, Community-acquired pneumoniaTie-Gang ZhangAi-Hua LiMin LyuMeng ChenFang HuangJiang WuKeAi Communications Co., Ltd.articleMedicine (General)R5-920ENChronic Diseases and Translational Medicine, Vol 1, Iss 2, Pp 110-116 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Tie-Gang Zhang Ai-Hua Li Min Lyu Meng Chen Fang Huang Jiang Wu Detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR
description	Objective: The aim of this study was to determine the etiology and prevalence of pediatric CAP in Beijing using a real-time polymerase chain reaction (PCR) technique. Methods: Between February 15, 2011 and January 18, 2012, 371 pediatric patients with CAP were enrolled at Beijing Children's Hospital. Sixteen respiratory viruses and two bacteria were detected from tracheal aspirate specimens using commercially available multiplex real-time reverse transcription PCR (RT-PCR) kits. Results: A single viral pathogen was detected in 35.3% of enrolled patients, multiple viruses in 11.6%, and virus/bacteria coinfection in 17.8%. In contrast, only 6.5% of patients had a single bacterial pathogen and 2.2% were infected with multiple bacteria. The etiological agent was unknown for 26.7% of patients. The most common viruses were respiratory syncytial virus (RSV) (43.9%), rhinovirus (14.8%), parainï¬uenza virus (9.4%), and adenovirus (8.6%). In patients under three years of age, RSV (44.6%), rhinovirus (12.8%), and Streptococcus pneumoniae (9.9%) were the most frequent pathogens. In children aged 3â7 years, S. pneumoniae (38.9%), RSV (30.6%), Haemophilus inï¬uenzae (19.4%), and adenovirus (19.4%) were most prevalent. Finally in children over seven years, RSV (47.3%), S. pneumoniae (41.9%), and rhinovirus (21.5%) infections were most frequent. Conclusions: Viral pathogens, specifically RSV, were responsible for the majority of CAP in pediatric patients. However, both S. pneumoniae and H. inï¬uenzae contributed as major causes of disease. Commercially available multiplexing real-time PCR allowed for rapid detection of the etiological agent. Keywords: Real-time reverse transcription polymerase chain reaction (RT-PCR), Respiratory virus, Community-acquired pneumonia
format	article
author	Tie-Gang Zhang Ai-Hua Li Min Lyu Meng Chen Fang Huang Jiang Wu
author_facet	Tie-Gang Zhang Ai-Hua Li Min Lyu Meng Chen Fang Huang Jiang Wu
author_sort	Tie-Gang Zhang
title	Detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR
title_short	Detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR
title_full	Detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR
title_fullStr	Detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR
title_full_unstemmed	Detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR
title_sort	detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in beijing using real-time pcr
publisher	KeAi Communications Co., Ltd.
publishDate	2015
url	https://doaj.org/article/85f215a60b614c1a8c9d2bbde41cf76e
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Detection of respiratory viral and bacterial pathogens causing pediatric community-acquired pneumonia in Beijing using real-time PCR

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