Effect of piR-9994 on Proliferation, Migration and Invasion of Gastric Cancer Cells and Its Mechanism

Objective To investigate the effect of piR-9994 on the biological behavior of gastric cancer cells and its possible mechanism. Methods The expression of piR-9994 in gastric cancer cell lines (MGC803 and AGS) and normal gastric epithelial cells (GES-1) were detected by qRT-PCR. MGC803 cell line with...

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Autores principales:	LIN Huamei, ZOU Changyan, SU Ying, HU Dan, LIAO Jinrong, LIN Keyu, HE Huocong, ZHENG Xiongwei, LIN Xiandong
Formato:	article
Lenguaje:	ZH
Publicado:	Magazine House of Cancer Research on Prevention and Treatment 2021
Materias:	gastric cancer pir-9994 proliferation invasion emt Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/85f46bba62694f5eb18ec09888cd909b
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spelling	oai:doaj.org-article:85f46bba62694f5eb18ec09888cd909b2021-11-05T02:22:52ZEffect of piR-9994 on Proliferation, Migration and Invasion of Gastric Cancer Cells and Its Mechanism1000-857810.3971/j.issn.1000-8578.2021.21.0468https://doaj.org/article/85f46bba62694f5eb18ec09888cd909b2021-10-01T00:00:00Zhttp://html.rhhz.net/ZLFZYJ/html/8578.2021.21.0468.htmhttps://doaj.org/toc/1000-8578Objective To investigate the effect of piR-9994 on the biological behavior of gastric cancer cells and its possible mechanism. Methods The expression of piR-9994 in gastric cancer cell lines (MGC803 and AGS) and normal gastric epithelial cells (GES-1) were detected by qRT-PCR. MGC803 cell line with piR-9994 overexpression and knockdown were constructed. The effects of piR-9994 expression changes on cell proliferation were detected by MTT and clone formation assay. The scratch wound healing assay and Transwell invasion assay were used to detect cell migration and invasion abilities. qRT-PCR and Western blot were used to detect cell proliferation and EMT-related genes expression. Results The expression level of piR-9994 in MGC803 cells was significantly higher than that in normal gastric epithelial cell line GES-1 (P < 0.05). The overexpression of piR-9994 promoted the proliferation, invasion and metastasis of gastric cancer cells, while piR-9994 knockdown had the opposite effect. piR-9994 expression was closely related to cell cycle, especially in S phase. The overexpression of piR-9994 promoted the expression of proliferation-related genes PCNA, CCND1 and Bcl-2, inhibited the expression of apoptosis gene C-PARP, and promoted the expression of EMT-related genes N-cadherin, MMP7, Twist and Vimentin; while piR-9994 knockdown had the opposite effect. Conclusion Abnormal expression of piR-9994 affects the proliferation, invasion, metastasis and EMT process of gastric cancer cells. piR-9994 may be a new biomarker and therapeutic target for gastric cancer.LIN HuameiZOU ChangyanSU YingHU DanLIAO JinrongLIN KeyuHE HuocongZHENG XiongweiLIN XiandongMagazine House of Cancer Research on Prevention and Treatmentarticlegastric cancerpir-9994proliferationinvasionemtNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ZHZhongliu Fangzhi Yanjiu, Vol 48, Iss 10, Pp 922-928 (2021)
institution	DOAJ
collection	DOAJ
language	ZH
topic	gastric cancer pir-9994 proliferation invasion emt Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	gastric cancer pir-9994 proliferation invasion emt Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 LIN Huamei ZOU Changyan SU Ying HU Dan LIAO Jinrong LIN Keyu HE Huocong ZHENG Xiongwei LIN Xiandong Effect of piR-9994 on Proliferation, Migration and Invasion of Gastric Cancer Cells and Its Mechanism
description	Objective To investigate the effect of piR-9994 on the biological behavior of gastric cancer cells and its possible mechanism. Methods The expression of piR-9994 in gastric cancer cell lines (MGC803 and AGS) and normal gastric epithelial cells (GES-1) were detected by qRT-PCR. MGC803 cell line with piR-9994 overexpression and knockdown were constructed. The effects of piR-9994 expression changes on cell proliferation were detected by MTT and clone formation assay. The scratch wound healing assay and Transwell invasion assay were used to detect cell migration and invasion abilities. qRT-PCR and Western blot were used to detect cell proliferation and EMT-related genes expression. Results The expression level of piR-9994 in MGC803 cells was significantly higher than that in normal gastric epithelial cell line GES-1 (P < 0.05). The overexpression of piR-9994 promoted the proliferation, invasion and metastasis of gastric cancer cells, while piR-9994 knockdown had the opposite effect. piR-9994 expression was closely related to cell cycle, especially in S phase. The overexpression of piR-9994 promoted the expression of proliferation-related genes PCNA, CCND1 and Bcl-2, inhibited the expression of apoptosis gene C-PARP, and promoted the expression of EMT-related genes N-cadherin, MMP7, Twist and Vimentin; while piR-9994 knockdown had the opposite effect. Conclusion Abnormal expression of piR-9994 affects the proliferation, invasion, metastasis and EMT process of gastric cancer cells. piR-9994 may be a new biomarker and therapeutic target for gastric cancer.
format	article
author	LIN Huamei ZOU Changyan SU Ying HU Dan LIAO Jinrong LIN Keyu HE Huocong ZHENG Xiongwei LIN Xiandong
author_facet	LIN Huamei ZOU Changyan SU Ying HU Dan LIAO Jinrong LIN Keyu HE Huocong ZHENG Xiongwei LIN Xiandong
author_sort	LIN Huamei
title	Effect of piR-9994 on Proliferation, Migration and Invasion of Gastric Cancer Cells and Its Mechanism
title_short	Effect of piR-9994 on Proliferation, Migration and Invasion of Gastric Cancer Cells and Its Mechanism
title_full	Effect of piR-9994 on Proliferation, Migration and Invasion of Gastric Cancer Cells and Its Mechanism
title_fullStr	Effect of piR-9994 on Proliferation, Migration and Invasion of Gastric Cancer Cells and Its Mechanism
title_full_unstemmed	Effect of piR-9994 on Proliferation, Migration and Invasion of Gastric Cancer Cells and Its Mechanism
title_sort	effect of pir-9994 on proliferation, migration and invasion of gastric cancer cells and its mechanism
publisher	Magazine House of Cancer Research on Prevention and Treatment
publishDate	2021
url	https://doaj.org/article/85f46bba62694f5eb18ec09888cd909b
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Effect of piR-9994 on Proliferation, Migration and Invasion of Gastric Cancer Cells and Its Mechanism

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