Evaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model

Despite the routine use of porcine reproductive and respiratory syndrome (PRRS)-modified live vaccines, serious concerns are currently being raised due to their quick reversion to virulence and limited cross-protection against divergent PRRS virus (PRRSV) strains circulating in the field. Therefore,...

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Autores principales:	Chang-Gi Jeong, Amina Khatun, Salik Nazki, Seung-Chai Kim, Yun-Hee Noh, Sang-Chul Kang, Dong-Uk Lee, Myeon-Sik Yang, Nadeem Shabir, In-Joong Yoon, Bumseok Kim, Won-Il Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	porcine reproductive and respiratory syndrome PRRSV reproductive model reproductive failure PRRS vaccine chimeric vaccine Medicine R
Acceso en línea:	https://doaj.org/article/85f57fdc5dda464e96872bd3e0bdd626
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spelling	oai:doaj.org-article:85f57fdc5dda464e96872bd3e0bdd6262021-11-25T19:10:37ZEvaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model10.3390/vaccines91112582076-393Xhttps://doaj.org/article/85f57fdc5dda464e96872bd3e0bdd6262021-10-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1258https://doaj.org/toc/2076-393XDespite the routine use of porcine reproductive and respiratory syndrome (PRRS)-modified live vaccines, serious concerns are currently being raised due to their quick reversion to virulence and limited cross-protection against divergent PRRS virus (PRRSV) strains circulating in the field. Therefore, a PRRS chimeric vaccine (JB1) was produced using a DNA-launched infectious clone by replacing open reading frames (ORFs) 3–6 with those from a mixture of two genetically different PRRSV2 strains (K07–2273 and K08–1054) and ORF1a with that from a mutation-resistant PRRSV strain (RVRp22) exhibiting an attenuated phenotype. To evaluate the safety and cross-protective efficacy of JB1 in a reproductive model, eight PRRS-negative pregnant sows were purchased and divided into four groups. Four sows in two of the groups were vaccinated with JB1, and the other 4 sows were untreated at gestational day 60. At gestational day 93, one vaccinated group and one nonvaccinated group each were challenged with either K07–2273 or K08–1054. All of the sows aborted or delivered until gestation day 115 (24 days post challenge), and the newborn piglets were observed up to the 28th day after birth, which was the end of the experiment. Overall, pregnant sows of the JB1-vaccinated groups showed no meaningful viremia after vaccination and significant reductions in viremia with K07–2273 and K08–1054, exhibiting significantly higher levels of serum virus-neutralizing antibodies than non-vaccinated sows. Moreover, the JB1-vaccinated groups did not exhibit any abortion due to vaccination and showed improved piglet viability and birth weight. The piglets from JB1-vaccinated sows displayed lower viral concentrations in serum and fewer lung lesions compared with those of the piglets from the nonvaccinated sows. Therefore, JB1 is a safe and effective vaccine candidate that confers simultaneous protection against two genetically different PRRSV strains.Chang-Gi JeongAmina KhatunSalik NazkiSeung-Chai KimYun-Hee NohSang-Chul KangDong-Uk LeeMyeon-Sik YangNadeem ShabirIn-Joong YoonBumseok KimWon-Il KimMDPI AGarticleporcine reproductive and respiratory syndromePRRSVreproductive modelreproductive failurePRRS vaccinechimeric vaccineMedicineRENVaccines, Vol 9, Iss 1258, p 1258 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	porcine reproductive and respiratory syndrome PRRSV reproductive model reproductive failure PRRS vaccine chimeric vaccine Medicine R
spellingShingle	porcine reproductive and respiratory syndrome PRRSV reproductive model reproductive failure PRRS vaccine chimeric vaccine Medicine R Chang-Gi Jeong Amina Khatun Salik Nazki Seung-Chai Kim Yun-Hee Noh Sang-Chul Kang Dong-Uk Lee Myeon-Sik Yang Nadeem Shabir In-Joong Yoon Bumseok Kim Won-Il Kim Evaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model
description	Despite the routine use of porcine reproductive and respiratory syndrome (PRRS)-modified live vaccines, serious concerns are currently being raised due to their quick reversion to virulence and limited cross-protection against divergent PRRS virus (PRRSV) strains circulating in the field. Therefore, a PRRS chimeric vaccine (JB1) was produced using a DNA-launched infectious clone by replacing open reading frames (ORFs) 3–6 with those from a mixture of two genetically different PRRSV2 strains (K07–2273 and K08–1054) and ORF1a with that from a mutation-resistant PRRSV strain (RVRp22) exhibiting an attenuated phenotype. To evaluate the safety and cross-protective efficacy of JB1 in a reproductive model, eight PRRS-negative pregnant sows were purchased and divided into four groups. Four sows in two of the groups were vaccinated with JB1, and the other 4 sows were untreated at gestational day 60. At gestational day 93, one vaccinated group and one nonvaccinated group each were challenged with either K07–2273 or K08–1054. All of the sows aborted or delivered until gestation day 115 (24 days post challenge), and the newborn piglets were observed up to the 28th day after birth, which was the end of the experiment. Overall, pregnant sows of the JB1-vaccinated groups showed no meaningful viremia after vaccination and significant reductions in viremia with K07–2273 and K08–1054, exhibiting significantly higher levels of serum virus-neutralizing antibodies than non-vaccinated sows. Moreover, the JB1-vaccinated groups did not exhibit any abortion due to vaccination and showed improved piglet viability and birth weight. The piglets from JB1-vaccinated sows displayed lower viral concentrations in serum and fewer lung lesions compared with those of the piglets from the nonvaccinated sows. Therefore, JB1 is a safe and effective vaccine candidate that confers simultaneous protection against two genetically different PRRSV strains.
format	article
author	Chang-Gi Jeong Amina Khatun Salik Nazki Seung-Chai Kim Yun-Hee Noh Sang-Chul Kang Dong-Uk Lee Myeon-Sik Yang Nadeem Shabir In-Joong Yoon Bumseok Kim Won-Il Kim
author_facet	Chang-Gi Jeong Amina Khatun Salik Nazki Seung-Chai Kim Yun-Hee Noh Sang-Chul Kang Dong-Uk Lee Myeon-Sik Yang Nadeem Shabir In-Joong Yoon Bumseok Kim Won-Il Kim
author_sort	Chang-Gi Jeong
title	Evaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model
title_short	Evaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model
title_full	Evaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model
title_fullStr	Evaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model
title_full_unstemmed	Evaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model
title_sort	evaluation of the cross-protective efficacy of a chimeric prrsv vaccine against two genetically diverse prrsv2 field strains in a reproductive model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/85f57fdc5dda464e96872bd3e0bdd626
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Evaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model

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