Human metallo-β-lactamase enzymes degrade penicillin

Abstract Nonribosomal peptides are assemblages, including antibiotics, of canonical amino acids and other molecules. β-lactam antibiotics act on bacterial cell walls and can be cleaved by β-lactamases. β-lactamase activity in humans has been neglected, even though eighteen enzymes have already been...

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Autores principales:	Seydina M. Diene, Lucile Pinault, Vivek Keshri, Nicholas Armstrong, Saber Khelaifia, Eric Chabrière, Gustavo Caetano-Anolles, Philippe Colson, Bernard La Scola, Jean-Marc Rolain, Pierre Pontarotti, Didier Raoult
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85f64b16e26b4baca5d5358296d9896c
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spelling	oai:doaj.org-article:85f64b16e26b4baca5d5358296d9896c2021-12-02T16:08:05ZHuman metallo-β-lactamase enzymes degrade penicillin10.1038/s41598-019-48723-y2045-2322https://doaj.org/article/85f64b16e26b4baca5d5358296d9896c2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48723-yhttps://doaj.org/toc/2045-2322Abstract Nonribosomal peptides are assemblages, including antibiotics, of canonical amino acids and other molecules. β-lactam antibiotics act on bacterial cell walls and can be cleaved by β-lactamases. β-lactamase activity in humans has been neglected, even though eighteen enzymes have already been annotated such in human genome. Their hydrolysis activities on antibiotics have not been previously investigated. Here, we report that human cells were able to digest penicillin and this activity was inhibited by β-lactamase inhibitor, i.e. sulbactam. Penicillin degradation in human cells was microbiologically demonstrated on Pneumococcus. We expressed a MBLAC2 human β-lactamase, known as an exosome biogenesis enzyme. It cleaved penicillin and was inhibited by sulbactam. Finally, β-lactamases are widely distributed, archaic, and have wide spectrum, including digesting anticancer and β-lactams, that can be then used as nutriments. The evidence of the other MBLAC2 role as a bona fide β-lactamase allows for reassessment of β-lactams and β-lactamases role in humans.Seydina M. DieneLucile PinaultVivek KeshriNicholas ArmstrongSaber KhelaifiaEric ChabrièreGustavo Caetano-AnollesPhilippe ColsonBernard La ScolaJean-Marc RolainPierre PontarottiDidier RaoultNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-7 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seydina M. Diene Lucile Pinault Vivek Keshri Nicholas Armstrong Saber Khelaifia Eric Chabrière Gustavo Caetano-Anolles Philippe Colson Bernard La Scola Jean-Marc Rolain Pierre Pontarotti Didier Raoult Human metallo-β-lactamase enzymes degrade penicillin
description	Abstract Nonribosomal peptides are assemblages, including antibiotics, of canonical amino acids and other molecules. β-lactam antibiotics act on bacterial cell walls and can be cleaved by β-lactamases. β-lactamase activity in humans has been neglected, even though eighteen enzymes have already been annotated such in human genome. Their hydrolysis activities on antibiotics have not been previously investigated. Here, we report that human cells were able to digest penicillin and this activity was inhibited by β-lactamase inhibitor, i.e. sulbactam. Penicillin degradation in human cells was microbiologically demonstrated on Pneumococcus. We expressed a MBLAC2 human β-lactamase, known as an exosome biogenesis enzyme. It cleaved penicillin and was inhibited by sulbactam. Finally, β-lactamases are widely distributed, archaic, and have wide spectrum, including digesting anticancer and β-lactams, that can be then used as nutriments. The evidence of the other MBLAC2 role as a bona fide β-lactamase allows for reassessment of β-lactams and β-lactamases role in humans.
format	article
author	Seydina M. Diene Lucile Pinault Vivek Keshri Nicholas Armstrong Saber Khelaifia Eric Chabrière Gustavo Caetano-Anolles Philippe Colson Bernard La Scola Jean-Marc Rolain Pierre Pontarotti Didier Raoult
author_facet	Seydina M. Diene Lucile Pinault Vivek Keshri Nicholas Armstrong Saber Khelaifia Eric Chabrière Gustavo Caetano-Anolles Philippe Colson Bernard La Scola Jean-Marc Rolain Pierre Pontarotti Didier Raoult
author_sort	Seydina M. Diene
title	Human metallo-β-lactamase enzymes degrade penicillin
title_short	Human metallo-β-lactamase enzymes degrade penicillin
title_full	Human metallo-β-lactamase enzymes degrade penicillin
title_fullStr	Human metallo-β-lactamase enzymes degrade penicillin
title_full_unstemmed	Human metallo-β-lactamase enzymes degrade penicillin
title_sort	human metallo-β-lactamase enzymes degrade penicillin
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/85f64b16e26b4baca5d5358296d9896c
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Human metallo-β-lactamase enzymes degrade penicillin

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