Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications

Alan IS Morais, 1,* Xichi Wang, 2–4,* Ewerton G Vieira, 1 Bartolomeu C Viana, 1, 5 Edson C Silva-Filho, 1 Josy A Osajima, 1 Samson Afewerki, 3, 4 Marcus AF Corat, 6 Heurison S Silva, 5 Fernanda R Marciano, 5 Guillermo U Ruiz-Esparza, 3, 4 Thiago D Stocco, 6, 7 Mirian MM de Paula, 6 Anderso...

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Autores principales:	Morais AIS, Wang X, Vieira EG, Viana BC, Silva-Filho EC, Osajima JA, Afewerki S, Corat MAF, Silva HS, Marciano FR, Ruiz-Esparza GU, Stocco TD, de Paula MMM, Lobo AO
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	oxygen-generating-microparticles electrospraying tissue engineering calcium peroxide cartilage Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/85f7392d9937473d97c95cf2a20658c9
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spelling	oai:doaj.org-article:85f7392d9937473d97c95cf2a20658c92021-12-02T07:19:32ZElectrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications1178-2013https://doaj.org/article/85f7392d9937473d97c95cf2a20658c92020-02-01T00:00:00Zhttps://www.dovepress.com/electrospraying-oxygen-generating-microparticles-for-tissue-engineerin-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Alan IS Morais, 1,* Xichi Wang, 2–4,* Ewerton G Vieira, 1 Bartolomeu C Viana, 1, 5 Edson C Silva-Filho, 1 Josy A Osajima, 1 Samson Afewerki, 3, 4 Marcus AF Corat, 6 Heurison S Silva, 5 Fernanda R Marciano, 5 Guillermo U Ruiz-Esparza, 3, 4 Thiago D Stocco, 6, 7 Mirian MM de Paula, 6 Anderson O Lobo 1 1LIMAV-Interdisciplinary Laboratory for Advanced Materials, Materials Science and Engineering Graduate Program, UFPI-Federal University of Piauí, Teresina, PI CEP 64049-550, Brazil; 2Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 3Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham & Women´s Hospital, Cambridge, MA 02139, USA; 4Harvard-MIT Division of Health Science and Technology, Massachusetts Institute of Technology, MIT, Cambridge, MA 02139, USA; 5Department of Physics, UFPI-Federal University of Piauí, Teresina, PI CEP 64049-550, Brazil; 6Multidisciplinary Center for Biological Research, University of Campinas (UNICAMP), Campinas 13083-877, Brazil; 7Faculty of Physiotherapy, Santo Amaro University, São Paulo 04829-300, Brazil*These authors contributed equally to this workCorrespondence: Anderson O Lobo Email lobo@ufpi.edu.brBackground: The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications.Methods: The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis.Results: The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ = – 16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M.Conclusion: This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.Keywords: oxygen-generating-microparticles, electrospraying, tissue engineering, calcium peroxide, cartilageMorais AISWang XVieira EGViana BCSilva-Filho ECOsajima JAAfewerki SCorat MAFSilva HSMarciano FRRuiz-Esparza GUStocco TDde Paula MMMLobo AODove Medical Pressarticleoxygen-generating-microparticleselectrosprayingtissue engineeringcalcium peroxidecartilageMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 1173-1186 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	oxygen-generating-microparticles electrospraying tissue engineering calcium peroxide cartilage Medicine (General) R5-920
spellingShingle	oxygen-generating-microparticles electrospraying tissue engineering calcium peroxide cartilage Medicine (General) R5-920 Morais AIS Wang X Vieira EG Viana BC Silva-Filho EC Osajima JA Afewerki S Corat MAF Silva HS Marciano FR Ruiz-Esparza GU Stocco TD de Paula MMM Lobo AO Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications
description	Alan IS Morais, 1,* Xichi Wang, 2–4,* Ewerton G Vieira, 1 Bartolomeu C Viana, 1, 5 Edson C Silva-Filho, 1 Josy A Osajima, 1 Samson Afewerki, 3, 4 Marcus AF Corat, 6 Heurison S Silva, 5 Fernanda R Marciano, 5 Guillermo U Ruiz-Esparza, 3, 4 Thiago D Stocco, 6, 7 Mirian MM de Paula, 6 Anderson O Lobo 1 1LIMAV-Interdisciplinary Laboratory for Advanced Materials, Materials Science and Engineering Graduate Program, UFPI-Federal University of Piauí, Teresina, PI CEP 64049-550, Brazil; 2Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 3Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham & Women´s Hospital, Cambridge, MA 02139, USA; 4Harvard-MIT Division of Health Science and Technology, Massachusetts Institute of Technology, MIT, Cambridge, MA 02139, USA; 5Department of Physics, UFPI-Federal University of Piauí, Teresina, PI CEP 64049-550, Brazil; 6Multidisciplinary Center for Biological Research, University of Campinas (UNICAMP), Campinas 13083-877, Brazil; 7Faculty of Physiotherapy, Santo Amaro University, São Paulo 04829-300, Brazil*These authors contributed equally to this workCorrespondence: Anderson O Lobo Email lobo@ufpi.edu.brBackground: The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications.Methods: The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis.Results: The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ = – 16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M.Conclusion: This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.Keywords: oxygen-generating-microparticles, electrospraying, tissue engineering, calcium peroxide, cartilage
format	article
author	Morais AIS Wang X Vieira EG Viana BC Silva-Filho EC Osajima JA Afewerki S Corat MAF Silva HS Marciano FR Ruiz-Esparza GU Stocco TD de Paula MMM Lobo AO
author_facet	Morais AIS Wang X Vieira EG Viana BC Silva-Filho EC Osajima JA Afewerki S Corat MAF Silva HS Marciano FR Ruiz-Esparza GU Stocco TD de Paula MMM Lobo AO
author_sort	Morais AIS
title	Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications
title_short	Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications
title_full	Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications
title_fullStr	Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications
title_full_unstemmed	Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications
title_sort	electrospraying oxygen-generating microparticles for tissue engineering applications
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/85f7392d9937473d97c95cf2a20658c9
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Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications

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