Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida

Abstract Potato cyst nematodes (PCNs), Globodera rostochiensis and G. pallida, cause important economic losses. They are hard to manage because of their ability to remain dormant in soil for many years. Although general knowledge about these plant parasitic nematodes has considerably increased over...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marc-Olivier Duceppe, Joël Lafond-Lapalme, Juan Emilio Palomares-Rius, Michaël Sabeh, Vivian Blok, Peter Moffett, Benjamin Mimee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/85f84cd9ccd74e6f91e3c4bae4b9da9c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:85f84cd9ccd74e6f91e3c4bae4b9da9c
record_format	dspace
spelling	oai:doaj.org-article:85f84cd9ccd74e6f91e3c4bae4b9da9c2021-12-02T12:32:17ZAnalysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida10.1038/s41598-017-03871-x2045-2322https://doaj.org/article/85f84cd9ccd74e6f91e3c4bae4b9da9c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03871-xhttps://doaj.org/toc/2045-2322Abstract Potato cyst nematodes (PCNs), Globodera rostochiensis and G. pallida, cause important economic losses. They are hard to manage because of their ability to remain dormant in soil for many years. Although general knowledge about these plant parasitic nematodes has considerably increased over the past decades, very little is known about molecular events involved in cyst dormancy and hatching, two key steps of their development. Here, we have studied the progression of PCN transcriptomes from dry cysts to hatched juveniles using RNA-Seq. We found that several cell detoxification-related genes were highly active in the dry cysts. Many genes linked to an increase of calcium and water uptake were up-regulated during transition from dormancy to hydration. Exposure of hydrated cysts to host plant root exudates resulted in different transcriptional response between species. After 48 h of exposure, G. pallida cysts showed no significant modulation of gene expression while G. rostochiensis had 278 differentially expressed genes. The first G. rostochiensis significantly up-regulated gene was observed after 8 h and was coding for a transmembrane metalloprotease. This enzyme is able to activate/inactivate peptide hormones and could be involved in a cascade of events leading to hatching. Several known effector genes were also up-regulated during hatching.Marc-Olivier DuceppeJoël Lafond-LapalmeJuan Emilio Palomares-RiusMichaël SabehVivian BlokPeter MoffettBenjamin MimeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marc-Olivier Duceppe Joël Lafond-Lapalme Juan Emilio Palomares-Rius Michaël Sabeh Vivian Blok Peter Moffett Benjamin Mimee Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida
description	Abstract Potato cyst nematodes (PCNs), Globodera rostochiensis and G. pallida, cause important economic losses. They are hard to manage because of their ability to remain dormant in soil for many years. Although general knowledge about these plant parasitic nematodes has considerably increased over the past decades, very little is known about molecular events involved in cyst dormancy and hatching, two key steps of their development. Here, we have studied the progression of PCN transcriptomes from dry cysts to hatched juveniles using RNA-Seq. We found that several cell detoxification-related genes were highly active in the dry cysts. Many genes linked to an increase of calcium and water uptake were up-regulated during transition from dormancy to hydration. Exposure of hydrated cysts to host plant root exudates resulted in different transcriptional response between species. After 48 h of exposure, G. pallida cysts showed no significant modulation of gene expression while G. rostochiensis had 278 differentially expressed genes. The first G. rostochiensis significantly up-regulated gene was observed after 8 h and was coding for a transmembrane metalloprotease. This enzyme is able to activate/inactivate peptide hormones and could be involved in a cascade of events leading to hatching. Several known effector genes were also up-regulated during hatching.
format	article
author	Marc-Olivier Duceppe Joël Lafond-Lapalme Juan Emilio Palomares-Rius Michaël Sabeh Vivian Blok Peter Moffett Benjamin Mimee
author_facet	Marc-Olivier Duceppe Joël Lafond-Lapalme Juan Emilio Palomares-Rius Michaël Sabeh Vivian Blok Peter Moffett Benjamin Mimee
author_sort	Marc-Olivier Duceppe
title	Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida
title_short	Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida
title_full	Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida
title_fullStr	Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida
title_full_unstemmed	Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida
title_sort	analysis of survival and hatching transcriptomes from potato cyst nematodes, globodera rostochiensis and g. pallida
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/85f84cd9ccd74e6f91e3c4bae4b9da9c
work_keys_str_mv	AT marcolivierduceppe analysisofsurvivalandhatchingtranscriptomesfrompotatocystnematodesgloboderarostochiensisandgpallida AT joellafondlapalme analysisofsurvivalandhatchingtranscriptomesfrompotatocystnematodesgloboderarostochiensisandgpallida AT juanemiliopalomaresrius analysisofsurvivalandhatchingtranscriptomesfrompotatocystnematodesgloboderarostochiensisandgpallida AT michaelsabeh analysisofsurvivalandhatchingtranscriptomesfrompotatocystnematodesgloboderarostochiensisandgpallida AT vivianblok analysisofsurvivalandhatchingtranscriptomesfrompotatocystnematodesgloboderarostochiensisandgpallida AT petermoffett analysisofsurvivalandhatchingtranscriptomesfrompotatocystnematodesgloboderarostochiensisandgpallida AT benjaminmimee analysisofsurvivalandhatchingtranscriptomesfrompotatocystnematodesgloboderarostochiensisandgpallida
_version_	1718394104776228864

Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida

Ejemplares similares