Genome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance

The nuclear factor Y (NF-Y) family is comprised of transcription factors that have been implicated in multiple plant biological processes. However, little is known about this family in potato. In the present study, a total of 41 StNF-Y genes were identified in the potato genome. In addition, the phy...

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Autores principales: Shigui Li, Ning Zhang, Xi Zhu, Rui Ma, Shengyan Liu, Xiao Wang, Jiangwei Yang, Huaijun Si
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Publicado: Frontiers Media S.A. 2021
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spelling oai:doaj.org-article:8603b2df78024b7b940005e8a37a11222021-11-10T16:32:10ZGenome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance1664-462X10.3389/fpls.2021.749688https://doaj.org/article/8603b2df78024b7b940005e8a37a11222021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.749688/fullhttps://doaj.org/toc/1664-462XThe nuclear factor Y (NF-Y) family is comprised of transcription factors that have been implicated in multiple plant biological processes. However, little is known about this family in potato. In the present study, a total of 41 StNF-Y genes were identified in the potato genome. In addition, the phylogenetic, gene structure, motif, and chromosomal location of this family were analyzed. The tissue expression profiles based on RNA-seq data showed that 27 StNF-Y genes had tissue-specific expression, while the remaining 14 had low expression in all tissues. Publicly available transcriptomics data from various abiotic stresses revealed several stress-responsive StNF-Y genes, which were further verified via quantitative real-time polymerase chain reaction experiments. Furthermore, the StNF-YC9 gene was highly induced by dehydration and drought treatments. StNF-YC9 protein was mainly localized in the nucleus and cytoplasmic membrane. Overexpressing StNF-YC9 potato lines (OxStNF-YC9) had significantly increased in root length and exhibited stronger stomatal closure in potato treated by polyethylene-glycol and abscisic acid. In addition, OxStNF-YC9 lines had higher photosynthetic rates and decreased water loss under short-term drought stress compared to wild-type plants. During long-term drought stress, OxStNF-YC9 lines had higher proline levels, lower malondialdehyde content, and increased activity of several antioxidant enzymes, including superoxide dismutase, catalase, and peroxidase. This study increased our understanding of the StNF-Y gene and suggested that StNF-YC9 played an important role in drought tolerance by increased the photosynthesis rate, antioxidant enzyme activity, and proline accumulation coupled to lowered malondialdehyde accumulation in potato.Shigui LiShigui LiNing ZhangNing ZhangXi ZhuRui MaShengyan LiuXiao WangJiangwei YangJiangwei YangHuaijun SiHuaijun SiFrontiers Media S.A.articleNF-Y transcription factorpotatobioinformaticsabiotic stressesroot lengthstomata closurePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution DOAJ
collection DOAJ
language EN
topic NF-Y transcription factor
potato
bioinformatics
abiotic stresses
root length
stomata closure
Plant culture
SB1-1110
spellingShingle NF-Y transcription factor
potato
bioinformatics
abiotic stresses
root length
stomata closure
Plant culture
SB1-1110
Shigui Li
Shigui Li
Ning Zhang
Ning Zhang
Xi Zhu
Rui Ma
Shengyan Liu
Xiao Wang
Jiangwei Yang
Jiangwei Yang
Huaijun Si
Huaijun Si
Genome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance
description The nuclear factor Y (NF-Y) family is comprised of transcription factors that have been implicated in multiple plant biological processes. However, little is known about this family in potato. In the present study, a total of 41 StNF-Y genes were identified in the potato genome. In addition, the phylogenetic, gene structure, motif, and chromosomal location of this family were analyzed. The tissue expression profiles based on RNA-seq data showed that 27 StNF-Y genes had tissue-specific expression, while the remaining 14 had low expression in all tissues. Publicly available transcriptomics data from various abiotic stresses revealed several stress-responsive StNF-Y genes, which were further verified via quantitative real-time polymerase chain reaction experiments. Furthermore, the StNF-YC9 gene was highly induced by dehydration and drought treatments. StNF-YC9 protein was mainly localized in the nucleus and cytoplasmic membrane. Overexpressing StNF-YC9 potato lines (OxStNF-YC9) had significantly increased in root length and exhibited stronger stomatal closure in potato treated by polyethylene-glycol and abscisic acid. In addition, OxStNF-YC9 lines had higher photosynthetic rates and decreased water loss under short-term drought stress compared to wild-type plants. During long-term drought stress, OxStNF-YC9 lines had higher proline levels, lower malondialdehyde content, and increased activity of several antioxidant enzymes, including superoxide dismutase, catalase, and peroxidase. This study increased our understanding of the StNF-Y gene and suggested that StNF-YC9 played an important role in drought tolerance by increased the photosynthesis rate, antioxidant enzyme activity, and proline accumulation coupled to lowered malondialdehyde accumulation in potato.
format article
author Shigui Li
Shigui Li
Ning Zhang
Ning Zhang
Xi Zhu
Rui Ma
Shengyan Liu
Xiao Wang
Jiangwei Yang
Jiangwei Yang
Huaijun Si
Huaijun Si
author_facet Shigui Li
Shigui Li
Ning Zhang
Ning Zhang
Xi Zhu
Rui Ma
Shengyan Liu
Xiao Wang
Jiangwei Yang
Jiangwei Yang
Huaijun Si
Huaijun Si
author_sort Shigui Li
title Genome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance
title_short Genome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance
title_full Genome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance
title_fullStr Genome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance
title_full_unstemmed Genome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance
title_sort genome-wide analysis of nf-y genes in potato and functional identification of stnf-yc9 in drought tolerance
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/8603b2df78024b7b940005e8a37a1122
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