Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.

The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-direct...

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Autores principales:	Yakov Korkhin, Ulug M Unligil, Otis Littlefield, Pamlea J Nelson, David I Stuart, Paul B Sigler, Stephen D Bell, Nicola G A Abrescia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8606eed1a0604377bdd45343053e2e4a
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spelling	oai:doaj.org-article:8606eed1a0604377bdd45343053e2e4a2021-12-02T19:54:29ZEvolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.1544-91731545-788510.1371/journal.pbio.1000102https://doaj.org/article/8606eed1a0604377bdd45343053e2e4a2009-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19419240/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.Yakov KorkhinUlug M UnligilOtis LittlefieldPamlea J NelsonDavid I StuartPaul B SiglerStephen D BellNicola G A AbresciaPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 7, Iss 5, p e1000102 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Yakov Korkhin Ulug M Unligil Otis Littlefield Pamlea J Nelson David I Stuart Paul B Sigler Stephen D Bell Nicola G A Abrescia Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.
description	The archaeal RNA polymerase (RNAP) shares structural similarities with eukaryotic RNAP II but requires a reduced subset of general transcription factors for promoter-dependent initiation. To deepen our knowledge of cellular transcription, we have determined the structure of the 13-subunit DNA-directed RNAP from Sulfolobus shibatae at 3.35 Å resolution. The structure contains the full complement of subunits, including RpoG/Rpb8 and the equivalent of the clamp-head and jaw domains of the eukaryotic Rpb1. Furthermore, we have identified subunit Rpo13, an RNAP component in the order Sulfolobales, which contains a helix-turn-helix motif that interacts with the RpoH/Rpb5 and RpoA'/Rpb1 subunits. Its location and topology suggest a role in the formation of the transcription bubble.
format	article
author	Yakov Korkhin Ulug M Unligil Otis Littlefield Pamlea J Nelson David I Stuart Paul B Sigler Stephen D Bell Nicola G A Abrescia
author_facet	Yakov Korkhin Ulug M Unligil Otis Littlefield Pamlea J Nelson David I Stuart Paul B Sigler Stephen D Bell Nicola G A Abrescia
author_sort	Yakov Korkhin
title	Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.
title_short	Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.
title_full	Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.
title_fullStr	Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.
title_full_unstemmed	Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.
title_sort	evolution of complex rna polymerases: the complete archaeal rna polymerase structure.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/8606eed1a0604377bdd45343053e2e4a
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Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.

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