Topological and functional characterization of an insect gustatory receptor.

Insect gustatory receptors are predicted to have a seven-transmembrane structure and are distantly related to insect olfactory receptors, which have an inverted topology compared with G-protein coupled receptors, including mammalian olfactory receptors. In contrast, the topology of insect gustatory...

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Autores principales:	Hui-Jie Zhang, Alisha R Anderson, Stephen C Trowell, A-Rong Luo, Zhong-Huai Xiang, Qing-You Xia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/86090bca72f34c6080aecb9eb064d64c
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spelling	oai:doaj.org-article:86090bca72f34c6080aecb9eb064d64c2021-11-18T06:47:05ZTopological and functional characterization of an insect gustatory receptor.1932-620310.1371/journal.pone.0024111https://doaj.org/article/86090bca72f34c6080aecb9eb064d64c2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21912618/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Insect gustatory receptors are predicted to have a seven-transmembrane structure and are distantly related to insect olfactory receptors, which have an inverted topology compared with G-protein coupled receptors, including mammalian olfactory receptors. In contrast, the topology of insect gustatory receptors remains unknown. Except for a few examples from Drosophila, the specificity of individual insect gustatory receptors is also unknown. In this study, the total number of identified gustatory receptors in Bombyx mori was expanded from 65 to 69. BmGr8, a silkmoth gustatory receptor from the sugar receptor subfamily, was expressed in insect cells. Membrane topology studies on BmGr8 indicate that, like insect olfactory receptors, it has an inverted topology relative to G protein-coupled receptors. An orphan GR from the bitter receptor family, BmGr53, yielded similar results. We infer, from the finding that two distantly related BmGrs have an intracellular N-terminus and an odd number of transmembrane spans, that this is likely to be a general topology for all insect gustatory receptors. We also show that BmGr8 functions independently in Sf9 cells and responds in a concentration-dependent manner to the polyalcohols myo-inositol and epi-inositol but not to a range of mono- and di-saccharides. BmGr8 is the first chemoreceptor shown to respond specifically to inositol, an important or essential nutrient for some Lepidoptera. The selectivity of BmGr8 responses is consistent with the known responses of one of the gustatory receptor neurons in the lateral styloconic sensilla of B. mori, which responds to myo-inositol and epi-inositol but not to allo-inositol.Hui-Jie ZhangAlisha R AndersonStephen C TrowellA-Rong LuoZhong-Huai XiangQing-You XiaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e24111 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hui-Jie Zhang Alisha R Anderson Stephen C Trowell A-Rong Luo Zhong-Huai Xiang Qing-You Xia Topological and functional characterization of an insect gustatory receptor.
description	Insect gustatory receptors are predicted to have a seven-transmembrane structure and are distantly related to insect olfactory receptors, which have an inverted topology compared with G-protein coupled receptors, including mammalian olfactory receptors. In contrast, the topology of insect gustatory receptors remains unknown. Except for a few examples from Drosophila, the specificity of individual insect gustatory receptors is also unknown. In this study, the total number of identified gustatory receptors in Bombyx mori was expanded from 65 to 69. BmGr8, a silkmoth gustatory receptor from the sugar receptor subfamily, was expressed in insect cells. Membrane topology studies on BmGr8 indicate that, like insect olfactory receptors, it has an inverted topology relative to G protein-coupled receptors. An orphan GR from the bitter receptor family, BmGr53, yielded similar results. We infer, from the finding that two distantly related BmGrs have an intracellular N-terminus and an odd number of transmembrane spans, that this is likely to be a general topology for all insect gustatory receptors. We also show that BmGr8 functions independently in Sf9 cells and responds in a concentration-dependent manner to the polyalcohols myo-inositol and epi-inositol but not to a range of mono- and di-saccharides. BmGr8 is the first chemoreceptor shown to respond specifically to inositol, an important or essential nutrient for some Lepidoptera. The selectivity of BmGr8 responses is consistent with the known responses of one of the gustatory receptor neurons in the lateral styloconic sensilla of B. mori, which responds to myo-inositol and epi-inositol but not to allo-inositol.
format	article
author	Hui-Jie Zhang Alisha R Anderson Stephen C Trowell A-Rong Luo Zhong-Huai Xiang Qing-You Xia
author_facet	Hui-Jie Zhang Alisha R Anderson Stephen C Trowell A-Rong Luo Zhong-Huai Xiang Qing-You Xia
author_sort	Hui-Jie Zhang
title	Topological and functional characterization of an insect gustatory receptor.
title_short	Topological and functional characterization of an insect gustatory receptor.
title_full	Topological and functional characterization of an insect gustatory receptor.
title_fullStr	Topological and functional characterization of an insect gustatory receptor.
title_full_unstemmed	Topological and functional characterization of an insect gustatory receptor.
title_sort	topological and functional characterization of an insect gustatory receptor.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/86090bca72f34c6080aecb9eb064d64c
work_keys_str_mv	AT huijiezhang topologicalandfunctionalcharacterizationofaninsectgustatoryreceptor AT alisharanderson topologicalandfunctionalcharacterizationofaninsectgustatoryreceptor AT stephenctrowell topologicalandfunctionalcharacterizationofaninsectgustatoryreceptor AT arongluo topologicalandfunctionalcharacterizationofaninsectgustatoryreceptor AT zhonghuaixiang topologicalandfunctionalcharacterizationofaninsectgustatoryreceptor AT qingyouxia topologicalandfunctionalcharacterizationofaninsectgustatoryreceptor
_version_	1718424427203395584

Topological and functional characterization of an insect gustatory receptor.

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