MALDI-TOF MS as a Novel Tool for the Estimation of Postmortem Interval in Liver Tissue Samples

Abstract Estimation of the postmortem interval (PMI) is a complicated task in forensic medicine, especially during homicide and unwitnessed death investigations. Many biological, chemical, and physical indicators can be used to determine the postmortem interval, but most are not accurate. Here, we p...

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Autores principales:	Chengzhi Li, Zhengdong Li, Ya Tuo, Dong Ma, Yan Shi, Qinghua Zhang, Xianyi Zhuo, Kaifei Deng, Yijiu Chen, Zhenyuan Wang, Ping Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8616f832e32c43cb968348743e60c871
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spelling	oai:doaj.org-article:8616f832e32c43cb968348743e60c8712021-12-02T15:05:02ZMALDI-TOF MS as a Novel Tool for the Estimation of Postmortem Interval in Liver Tissue Samples10.1038/s41598-017-05216-02045-2322https://doaj.org/article/8616f832e32c43cb968348743e60c8712017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05216-0https://doaj.org/toc/2045-2322Abstract Estimation of the postmortem interval (PMI) is a complicated task in forensic medicine, especially during homicide and unwitnessed death investigations. Many biological, chemical, and physical indicators can be used to determine the postmortem interval, but most are not accurate. Here, we present a novel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method that can be used for the estimation of PMI using molecular images and multivariate analyses. In this study, we demonstrate that both rat and human liver tissues of various PMIs (0, 2, 4, and 6days) can be discriminated using MALDI imaging and principal component analysis (PCA). Using genetic algorithm (GA), supervised neural network (SNN), and quick classifier (QC) methods, we built 6 classification models, which showed high recognition capability and good cross-validation. The histological changes in all the samples at different time points were also consistent with the changes seen in MALDI imaging. Our work suggests that MALDI-TOF MS, along with multivariate analysis, can be used to determine intermediate PMIs.Chengzhi LiZhengdong LiYa TuoDong MaYan ShiQinghua ZhangXianyi ZhuoKaifei DengYijiu ChenZhenyuan WangPing HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chengzhi Li Zhengdong Li Ya Tuo Dong Ma Yan Shi Qinghua Zhang Xianyi Zhuo Kaifei Deng Yijiu Chen Zhenyuan Wang Ping Huang MALDI-TOF MS as a Novel Tool for the Estimation of Postmortem Interval in Liver Tissue Samples
description	Abstract Estimation of the postmortem interval (PMI) is a complicated task in forensic medicine, especially during homicide and unwitnessed death investigations. Many biological, chemical, and physical indicators can be used to determine the postmortem interval, but most are not accurate. Here, we present a novel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method that can be used for the estimation of PMI using molecular images and multivariate analyses. In this study, we demonstrate that both rat and human liver tissues of various PMIs (0, 2, 4, and 6days) can be discriminated using MALDI imaging and principal component analysis (PCA). Using genetic algorithm (GA), supervised neural network (SNN), and quick classifier (QC) methods, we built 6 classification models, which showed high recognition capability and good cross-validation. The histological changes in all the samples at different time points were also consistent with the changes seen in MALDI imaging. Our work suggests that MALDI-TOF MS, along with multivariate analysis, can be used to determine intermediate PMIs.
format	article
author	Chengzhi Li Zhengdong Li Ya Tuo Dong Ma Yan Shi Qinghua Zhang Xianyi Zhuo Kaifei Deng Yijiu Chen Zhenyuan Wang Ping Huang
author_facet	Chengzhi Li Zhengdong Li Ya Tuo Dong Ma Yan Shi Qinghua Zhang Xianyi Zhuo Kaifei Deng Yijiu Chen Zhenyuan Wang Ping Huang
author_sort	Chengzhi Li
title	MALDI-TOF MS as a Novel Tool for the Estimation of Postmortem Interval in Liver Tissue Samples
title_short	MALDI-TOF MS as a Novel Tool for the Estimation of Postmortem Interval in Liver Tissue Samples
title_full	MALDI-TOF MS as a Novel Tool for the Estimation of Postmortem Interval in Liver Tissue Samples
title_fullStr	MALDI-TOF MS as a Novel Tool for the Estimation of Postmortem Interval in Liver Tissue Samples
title_full_unstemmed	MALDI-TOF MS as a Novel Tool for the Estimation of Postmortem Interval in Liver Tissue Samples
title_sort	maldi-tof ms as a novel tool for the estimation of postmortem interval in liver tissue samples
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8616f832e32c43cb968348743e60c871
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MALDI-TOF MS as a Novel Tool for the Estimation of Postmortem Interval in Liver Tissue Samples

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