Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option

Abstract Lithocholic bile acid (LCA) has been reported to selectively kill cancer cells within many tumor cell lines including neuroblastoma or glioblastoma. Wilms’ tumor shares similarities with neuro- and glioblastoma. Hence, the aim of the study was to evaluate the effects of LCA on nephroblastom...

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Autores principales:	Julian Trah, Jonas Arand, Jun Oh, Laia Pagerols-Raluy, Magdalena Trochimiuk, Birgit Appl, Hannah Heidelbach, Deirdre Vincent, Moin A. Saleem, Konrad Reinshagen, Anne K. Mühlig, Michael Boettcher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/861855d3ccca4b3b99dc20358a396b22
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spelling	oai:doaj.org-article:861855d3ccca4b3b99dc20358a396b222021-12-02T11:41:50ZLithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option10.1038/s41598-020-77436-w2045-2322https://doaj.org/article/861855d3ccca4b3b99dc20358a396b222020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77436-whttps://doaj.org/toc/2045-2322Abstract Lithocholic bile acid (LCA) has been reported to selectively kill cancer cells within many tumor cell lines including neuroblastoma or glioblastoma. Wilms’ tumor shares similarities with neuro- and glioblastoma. Hence, the aim of the study was to evaluate the effects of LCA on nephroblastoma. To test the effects of LCA, nephroblastoma cell line WT CLS1 was used. SK NEP1 was tested as well. It was originally classified as a nephroblastoma cell line but was meanwhile reclassified as an ewing sarcoma cell line. As control cell lines HEK 293 from embryonic kidney and RC 124 from adult kidney tissue as well as podocytes were used. The effects were evaluated using proliferation assay, caspase activity assay, FACS and Western blot. LCA showed a dose and time-dependent selective effect inducing apoptosis in nephroblastoma cells. However, these effects were not limited to the nephroblastoma cell line but also affected control kidney cell lines and the sarcoma cells; only podocytes are significantly less affected by LCA (at dosages < 200 µm). There were no significant differences regarding the TGR5 receptor expression. The study showed that LCA has a strong, yet unselective effect on all used in vitro cell-lines, sparing the highly differentiated podocytes in lower concentrations. Further studies are needed to verify our results before dismissing LCA as an anti-cancer drug.Julian TrahJonas ArandJun OhLaia Pagerols-RaluyMagdalena TrochimiukBirgit ApplHannah HeidelbachDeirdre VincentMoin A. SaleemKonrad ReinshagenAnne K. MühligMichael BoettcherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julian Trah Jonas Arand Jun Oh Laia Pagerols-Raluy Magdalena Trochimiuk Birgit Appl Hannah Heidelbach Deirdre Vincent Moin A. Saleem Konrad Reinshagen Anne K. Mühlig Michael Boettcher Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option
description	Abstract Lithocholic bile acid (LCA) has been reported to selectively kill cancer cells within many tumor cell lines including neuroblastoma or glioblastoma. Wilms’ tumor shares similarities with neuro- and glioblastoma. Hence, the aim of the study was to evaluate the effects of LCA on nephroblastoma. To test the effects of LCA, nephroblastoma cell line WT CLS1 was used. SK NEP1 was tested as well. It was originally classified as a nephroblastoma cell line but was meanwhile reclassified as an ewing sarcoma cell line. As control cell lines HEK 293 from embryonic kidney and RC 124 from adult kidney tissue as well as podocytes were used. The effects were evaluated using proliferation assay, caspase activity assay, FACS and Western blot. LCA showed a dose and time-dependent selective effect inducing apoptosis in nephroblastoma cells. However, these effects were not limited to the nephroblastoma cell line but also affected control kidney cell lines and the sarcoma cells; only podocytes are significantly less affected by LCA (at dosages < 200 µm). There were no significant differences regarding the TGR5 receptor expression. The study showed that LCA has a strong, yet unselective effect on all used in vitro cell-lines, sparing the highly differentiated podocytes in lower concentrations. Further studies are needed to verify our results before dismissing LCA as an anti-cancer drug.
format	article
author	Julian Trah Jonas Arand Jun Oh Laia Pagerols-Raluy Magdalena Trochimiuk Birgit Appl Hannah Heidelbach Deirdre Vincent Moin A. Saleem Konrad Reinshagen Anne K. Mühlig Michael Boettcher
author_facet	Julian Trah Jonas Arand Jun Oh Laia Pagerols-Raluy Magdalena Trochimiuk Birgit Appl Hannah Heidelbach Deirdre Vincent Moin A. Saleem Konrad Reinshagen Anne K. Mühlig Michael Boettcher
author_sort	Julian Trah
title	Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option
title_short	Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option
title_full	Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option
title_fullStr	Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option
title_full_unstemmed	Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option
title_sort	lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/861855d3ccca4b3b99dc20358a396b22
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Lithocholic bile acid induces apoptosis in human nephroblastoma cells: a non-selective treatment option

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