Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation

(1) Background: Chitooligosaccharides (COS) have numerous applications due to their excellent properties. Chitosan hydrolysis using chitosanases has been proposed as an advisable method for COS preparation. Although many chitosanases from various sources have been identified, the cold-adapted ones w...

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Autores principales:	Lin-Lin Zhang, Xiao-Hua Jiang, Xin-Feng Xiao, Wen-Xiu Zhang, Yi-Qian Shi, Zhi-Peng Wang, Hai-Xiang Zhou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chitosanase cold adaptation chitooligosaccharide <i>Renibacterium</i> sp. Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8629524aad324803a6596d67ba7cb4b3
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spelling	oai:doaj.org-article:8629524aad324803a6596d67ba7cb4b32021-11-25T18:12:41ZExpression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation10.3390/md191105961660-3397https://doaj.org/article/8629524aad324803a6596d67ba7cb4b32021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/596https://doaj.org/toc/1660-3397(1) Background: Chitooligosaccharides (COS) have numerous applications due to their excellent properties. Chitosan hydrolysis using chitosanases has been proposed as an advisable method for COS preparation. Although many chitosanases from various sources have been identified, the cold-adapted ones with high stability are still rather rare but required. (2) Methods: A novel chitosanase named CsnY from marine bacterium <i>Renibacterium</i> sp. Y82 was expressed in <i>Escherichia coli</i>, following sequence analysis. Then, the characterizations of recombinant CsnY purified through Ni–NTA affinity chromatography were conducted, including effects of pH and temperature, effects of metal ions and chemicals, and final product analysis. (3) Results: The GH46 family chitosanase CsnY possessed promising thermostability at broad temperature range (0–50 °C), and with optimal activity at 40 °C and pH 6.0, especially showing relatively high activity (over 80% of its maximum activity) at low temperatures (20–30 °C), which demonstrated the cold-adapted property. Common metal ions or chemicals had no obvious effect on CsnY except Mn<sup>2+</sup> and Co<sup>2+</sup>. Finally, CsnY was determined to be an endo-type chitosanase generating chitodisaccharides and -trisaccharides as main products, whose total concentration reached 56.74 mM within 2 h against 2% (<i>w</i>/<i>v</i>) initial chitosan substrate. (4) Conclusions: The results suggest the cold-adapted CsnY with favorable stability has desirable potential for the industrial production of COS.Lin-Lin ZhangXiao-Hua JiangXin-Feng XiaoWen-Xiu ZhangYi-Qian ShiZhi-Peng WangHai-Xiang ZhouMDPI AGarticlechitosanasecold adaptationchitooligosaccharide<i>Renibacterium</i> sp.Biology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 596, p 596 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chitosanase cold adaptation chitooligosaccharide <i>Renibacterium</i> sp. Biology (General) QH301-705.5
spellingShingle	chitosanase cold adaptation chitooligosaccharide <i>Renibacterium</i> sp. Biology (General) QH301-705.5 Lin-Lin Zhang Xiao-Hua Jiang Xin-Feng Xiao Wen-Xiu Zhang Yi-Qian Shi Zhi-Peng Wang Hai-Xiang Zhou Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation
description	(1) Background: Chitooligosaccharides (COS) have numerous applications due to their excellent properties. Chitosan hydrolysis using chitosanases has been proposed as an advisable method for COS preparation. Although many chitosanases from various sources have been identified, the cold-adapted ones with high stability are still rather rare but required. (2) Methods: A novel chitosanase named CsnY from marine bacterium <i>Renibacterium</i> sp. Y82 was expressed in <i>Escherichia coli</i>, following sequence analysis. Then, the characterizations of recombinant CsnY purified through Ni–NTA affinity chromatography were conducted, including effects of pH and temperature, effects of metal ions and chemicals, and final product analysis. (3) Results: The GH46 family chitosanase CsnY possessed promising thermostability at broad temperature range (0–50 °C), and with optimal activity at 40 °C and pH 6.0, especially showing relatively high activity (over 80% of its maximum activity) at low temperatures (20–30 °C), which demonstrated the cold-adapted property. Common metal ions or chemicals had no obvious effect on CsnY except Mn<sup>2+</sup> and Co<sup>2+</sup>. Finally, CsnY was determined to be an endo-type chitosanase generating chitodisaccharides and -trisaccharides as main products, whose total concentration reached 56.74 mM within 2 h against 2% (<i>w</i>/<i>v</i>) initial chitosan substrate. (4) Conclusions: The results suggest the cold-adapted CsnY with favorable stability has desirable potential for the industrial production of COS.
format	article
author	Lin-Lin Zhang Xiao-Hua Jiang Xin-Feng Xiao Wen-Xiu Zhang Yi-Qian Shi Zhi-Peng Wang Hai-Xiang Zhou
author_facet	Lin-Lin Zhang Xiao-Hua Jiang Xin-Feng Xiao Wen-Xiu Zhang Yi-Qian Shi Zhi-Peng Wang Hai-Xiang Zhou
author_sort	Lin-Lin Zhang
title	Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation
title_short	Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation
title_full	Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation
title_fullStr	Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation
title_full_unstemmed	Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation
title_sort	expression and characterization of a novel cold-adapted chitosanase from marine <i>renibacterium</i> sp. suitable for chitooligosaccharides preparation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8629524aad324803a6596d67ba7cb4b3
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Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine <i>Renibacterium</i> sp. Suitable for Chitooligosaccharides Preparation

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