Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation

Abstract In vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Karina Cañón-Beltrán, Yulia N. Cajas, Serafín Peréz-Cerezales, Claudia L. V. Leal, Ekaitz Agirregoitia, Alfonso Gutierrez-Adán, Encina M. González, Dimitrios Rizos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/863266392d2849c2a7e3fe5948454646
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:863266392d2849c2a7e3fe5948454646
record_format	dspace
spelling	oai:doaj.org-article:863266392d2849c2a7e3fe59484546462021-12-02T15:03:05ZNobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation10.1038/s41598-021-91158-72045-2322https://doaj.org/article/863266392d2849c2a7e3fe59484546462021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91158-7https://doaj.org/toc/2045-2322Abstract In vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.Karina Cañón-BeltránYulia N. CajasSerafín Peréz-CerezalesClaudia L. V. LealEkaitz AgirregoitiaAlfonso Gutierrez-AdánEncina M. GonzálezDimitrios RizosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karina Cañón-Beltrán Yulia N. Cajas Serafín Peréz-Cerezales Claudia L. V. Leal Ekaitz Agirregoitia Alfonso Gutierrez-Adán Encina M. González Dimitrios Rizos Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation
description	Abstract In vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.
format	article
author	Karina Cañón-Beltrán Yulia N. Cajas Serafín Peréz-Cerezales Claudia L. V. Leal Ekaitz Agirregoitia Alfonso Gutierrez-Adán Encina M. González Dimitrios Rizos
author_facet	Karina Cañón-Beltrán Yulia N. Cajas Serafín Peréz-Cerezales Claudia L. V. Leal Ekaitz Agirregoitia Alfonso Gutierrez-Adán Encina M. González Dimitrios Rizos
author_sort	Karina Cañón-Beltrán
title	Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation
title_short	Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation
title_full	Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation
title_fullStr	Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation
title_full_unstemmed	Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation
title_sort	nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/863266392d2849c2a7e3fe5948454646
work_keys_str_mv	AT karinacanonbeltran nobiletinenhancesthedevelopmentandqualityofbovineembryosinvitroduringtwokeyperiodsofembryonicgenomeactivation AT yuliancajas nobiletinenhancesthedevelopmentandqualityofbovineembryosinvitroduringtwokeyperiodsofembryonicgenomeactivation AT serafinperezcerezales nobiletinenhancesthedevelopmentandqualityofbovineembryosinvitroduringtwokeyperiodsofembryonicgenomeactivation AT claudialvleal nobiletinenhancesthedevelopmentandqualityofbovineembryosinvitroduringtwokeyperiodsofembryonicgenomeactivation AT ekaitzagirregoitia nobiletinenhancesthedevelopmentandqualityofbovineembryosinvitroduringtwokeyperiodsofembryonicgenomeactivation AT alfonsogutierrezadan nobiletinenhancesthedevelopmentandqualityofbovineembryosinvitroduringtwokeyperiodsofembryonicgenomeactivation AT encinamgonzalez nobiletinenhancesthedevelopmentandqualityofbovineembryosinvitroduringtwokeyperiodsofembryonicgenomeactivation AT dimitriosrizos nobiletinenhancesthedevelopmentandqualityofbovineembryosinvitroduringtwokeyperiodsofembryonicgenomeactivation
_version_	1718389087745867776

Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation

Ejemplares similares