Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation

Cen Chen,1,2 Han Li,1 Xiangdong Kong,2 Sheng-Min Zhang,1 In-Seop Lee2,3 1Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, People’s Republic of China; 2Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou,...

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Autores principales:	Chen C, Li H, Kong XD, Zhang SM, Lee IS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/865143e569ed455f8d3933643890411b
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spelling	oai:doaj.org-article:865143e569ed455f8d3933643890411b2021-12-02T00:39:10ZImmobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation1178-2013https://doaj.org/article/865143e569ed455f8d3933643890411b2014-12-01T00:00:00Zhttp://www.dovepress.com/immobilizing-osteogenic-growth-peptide-with-and-without-fibronectin-on-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Cen Chen,1,2 Han Li,1 Xiangdong Kong,2 Sheng-Min Zhang,1 In-Seop Lee2,3 1Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, People’s Republic of China; 2Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People’s Republic of China; 3Institute of Natural Sciences, Yonsei University, Seoul, Korea Abstract: In this study, to improve the osseointegration of implants, osteogenic growth peptide (OGP) and fibronectin (FN) were loaded within mineral, which was formed on titanium, through adsorption and coprecipitation methods. The release profiles of OGP loaded by either adsorption or coprecipitation and the effects of the loading methods to immobilize OGP with and without FN on rat mesenchymal stem cell (rMSC) osteogenic differentiation were studied. The coprecipitation approach slightly reduced the initial burst release, while the adsorption approach provided a more sustained release. Dual loading of OGP and FN further improved cell attachments compared with either OGP or FN alone. Dually loaded OGP and FN also had a positive impact on rMSC proliferation and osteogenic differentiation. The difference in methods of loading OGP with and without FN also had some effects on osteogenic differentiation. Compared with coprecipitated OGP alone, adsorbed OGP enhanced later differentiation, such as osteocalcin secretion and matrix mineralization. Simultaneously adsorbed OGP and FN led to higher proliferation and higher osteogenic differentiation in both early and late stages compared with sequentially loaded OGP and FN. rMSC culture clearly indicated that simultaneously adsorbed OGP and FN could improve osseointegration, and this treatment represents a potential method for effective surface modification of dental and orthopedic implants. Keywords: coprecipitation, adsorption, osseointegrationChen CLi HKong XDZhang SMLee ISDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 283-295 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen C Li H Kong XD Zhang SM Lee IS Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation
description	Cen Chen,1,2 Han Li,1 Xiangdong Kong,2 Sheng-Min Zhang,1 In-Seop Lee2,3 1Advanced Biomaterials and Tissue Engineering Center, Huazhong University of Science and Technology, Wuhan, People’s Republic of China; 2Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People’s Republic of China; 3Institute of Natural Sciences, Yonsei University, Seoul, Korea Abstract: In this study, to improve the osseointegration of implants, osteogenic growth peptide (OGP) and fibronectin (FN) were loaded within mineral, which was formed on titanium, through adsorption and coprecipitation methods. The release profiles of OGP loaded by either adsorption or coprecipitation and the effects of the loading methods to immobilize OGP with and without FN on rat mesenchymal stem cell (rMSC) osteogenic differentiation were studied. The coprecipitation approach slightly reduced the initial burst release, while the adsorption approach provided a more sustained release. Dual loading of OGP and FN further improved cell attachments compared with either OGP or FN alone. Dually loaded OGP and FN also had a positive impact on rMSC proliferation and osteogenic differentiation. The difference in methods of loading OGP with and without FN also had some effects on osteogenic differentiation. Compared with coprecipitated OGP alone, adsorbed OGP enhanced later differentiation, such as osteocalcin secretion and matrix mineralization. Simultaneously adsorbed OGP and FN led to higher proliferation and higher osteogenic differentiation in both early and late stages compared with sequentially loaded OGP and FN. rMSC culture clearly indicated that simultaneously adsorbed OGP and FN could improve osseointegration, and this treatment represents a potential method for effective surface modification of dental and orthopedic implants. Keywords: coprecipitation, adsorption, osseointegration
format	article
author	Chen C Li H Kong XD Zhang SM Lee IS
author_facet	Chen C Li H Kong XD Zhang SM Lee IS
author_sort	Chen C
title	Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation
title_short	Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation
title_full	Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation
title_fullStr	Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation
title_full_unstemmed	Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation
title_sort	immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/865143e569ed455f8d3933643890411b
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Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation

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