Transcriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants

Abstract Candida albicans is a diploid fungus and a predominant opportunistic human pathogen. Notably, C. albicans employs reversible chromosomal aneuploidies as a means of survival in adverse environments. We previously characterized transcription on the monosomic chromosome 5 (Ch5) that arises wit...

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Autores principales:	Christopher Tucker, Soumyaroop Bhattacharya, Hironao Wakabayashi, Stanislav Bellaousov, Anatoliy Kravets, Stephen L. Welle, Jason Myers, Jeffrey J. Hayes, Michael Bulger, Elena Rustchenko
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/865206d6096f4bdf8a6fbb1ffa550712
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spelling	oai:doaj.org-article:865206d6096f4bdf8a6fbb1ffa5507122021-12-02T11:41:24ZTranscriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants10.1038/s41598-018-20106-92045-2322https://doaj.org/article/865206d6096f4bdf8a6fbb1ffa5507122018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20106-9https://doaj.org/toc/2045-2322Abstract Candida albicans is a diploid fungus and a predominant opportunistic human pathogen. Notably, C. albicans employs reversible chromosomal aneuploidies as a means of survival in adverse environments. We previously characterized transcription on the monosomic chromosome 5 (Ch5) that arises with adaptation to growth on the toxic sugar sorbose in the mutant Sor125(55). We now extend this analysis to the trisomic hybrid Ch4/7 within Sor125(55) and a diverse group of three mutants harboring a single Ch5. We find a similar pattern of transcriptional changes on either type of aneuploid chromosome within these mutants wherein expression of many genes follows chromosome ploidy, consistent with a direct mechanism to regulate genes important for adaptation to growth. In contrast, a significant number of genes are expressed at the disomic level, implying distinct mechanisms compensating for gene dose on monosomic or trisomic chromosomes consistent with maintaining cell homeostasis. Finally, we find evidence for an additional mechanism that elevates expression of genes on normal disomic Ch4 and Ch7 in mutants to levels commensurate with that found on the trisomic Ch4/7b in Sor125(55). Several of these genes are similarly differentially regulated among mutants, suggesting they play key functions in either maintaining aneuploidy or adaptation to growth conditions.Christopher TuckerSoumyaroop BhattacharyaHironao WakabayashiStanislav BellaousovAnatoliy KravetsStephen L. WelleJason MyersJeffrey J. HayesMichael BulgerElena RustchenkoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christopher Tucker Soumyaroop Bhattacharya Hironao Wakabayashi Stanislav Bellaousov Anatoliy Kravets Stephen L. Welle Jason Myers Jeffrey J. Hayes Michael Bulger Elena Rustchenko Transcriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants
description	Abstract Candida albicans is a diploid fungus and a predominant opportunistic human pathogen. Notably, C. albicans employs reversible chromosomal aneuploidies as a means of survival in adverse environments. We previously characterized transcription on the monosomic chromosome 5 (Ch5) that arises with adaptation to growth on the toxic sugar sorbose in the mutant Sor125(55). We now extend this analysis to the trisomic hybrid Ch4/7 within Sor125(55) and a diverse group of three mutants harboring a single Ch5. We find a similar pattern of transcriptional changes on either type of aneuploid chromosome within these mutants wherein expression of many genes follows chromosome ploidy, consistent with a direct mechanism to regulate genes important for adaptation to growth. In contrast, a significant number of genes are expressed at the disomic level, implying distinct mechanisms compensating for gene dose on monosomic or trisomic chromosomes consistent with maintaining cell homeostasis. Finally, we find evidence for an additional mechanism that elevates expression of genes on normal disomic Ch4 and Ch7 in mutants to levels commensurate with that found on the trisomic Ch4/7b in Sor125(55). Several of these genes are similarly differentially regulated among mutants, suggesting they play key functions in either maintaining aneuploidy or adaptation to growth conditions.
format	article
author	Christopher Tucker Soumyaroop Bhattacharya Hironao Wakabayashi Stanislav Bellaousov Anatoliy Kravets Stephen L. Welle Jason Myers Jeffrey J. Hayes Michael Bulger Elena Rustchenko
author_facet	Christopher Tucker Soumyaroop Bhattacharya Hironao Wakabayashi Stanislav Bellaousov Anatoliy Kravets Stephen L. Welle Jason Myers Jeffrey J. Hayes Michael Bulger Elena Rustchenko
author_sort	Christopher Tucker
title	Transcriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants
title_short	Transcriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants
title_full	Transcriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants
title_fullStr	Transcriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants
title_full_unstemmed	Transcriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants
title_sort	transcriptional regulation on aneuploid chromosomes in diverse candida albicans mutants
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/865206d6096f4bdf8a6fbb1ffa550712
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Transcriptional Regulation on Aneuploid Chromosomes in Diverse Candida albicans Mutants

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