Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles

Nagaraja SreeHarsha,1 Ravindra V Ghorpade,2 Abdullah Mossa Alzahrani,3 Bandar E Al-Dhubiab,1 Katharigatta N Venugopala1,41Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, Saudi Arabia; 2Chemical Engineering and Process Development Division, CSIR-N...

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Autores principales:	SreeHarsha N, Ghorpade RV, Alzahrani AM, Al-Dhubiab BE, Venugopala KN
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Nanoparticles Candida Antarctica Lipase B grafting immobilization Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8656e96c9333486092f09de4a536a429
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spelling	oai:doaj.org-article:8656e96c9333486092f09de4a536a4292021-12-02T07:22:07ZImmobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles1178-2013https://doaj.org/article/8656e96c9333486092f09de4a536a4292019-05-01T00:00:00Zhttps://www.dovepress.com/immobilization-studies-of-candida-antarctica-lipase-b-on-gallic-acid-r-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Nagaraja SreeHarsha,1 Ravindra V Ghorpade,2 Abdullah Mossa Alzahrani,3 Bandar E Al-Dhubiab,1 Katharigatta N Venugopala1,41Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, Saudi Arabia; 2Chemical Engineering and Process Development Division, CSIR-National Chemical Laboratory, Pune 411 008, India; 3Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia; 4Department of Biotechnology and Food Technology, Durban University of Technology, Durban 4001, South AfricaPurpose: Here, we present the successful preparation of a highly efficient gallic acid resin grafted with magnetic nanoparticles (MNPs) and containing a branched brush polymeric shell.Methods: Using a convenient co-precipitation method, we prepared Fe3O4 nanoparticles stabilized by citric acid. These nanoparticles underwent further silica modification and amino functionalization followed by gallic acid functionalization on their surface. Under alkaline conditions, we used a condensation reaction that combined formaldehyde and gallic, to graft the gallic acid−formaldehyde resin on the surface. We then evaluated the polymer-grafted MNPs to assay the Candida Antarctica B lipase(Cal-B) immobilization via physical adsorption.Conclusion: Furthermore, during optimization of parameters that defined conditions of immobilization, we found that the optimum immobilization was achieved in 15 mins. Also, optimal immobilization temperature and pH were 38ºC and 7.5, respectively. In addition, the reusability study of immobilized lipase polymer-grafted MNPs was done by isolating the MNPs from the reaction medium using magnetic separation, which showed that grafted MNPs reached 5 cycles with 91% activity retention.Keywords: nanoparticles, Candida Antarctica lipase B, grafting, immobilizationSreeHarsha NGhorpade RVAlzahrani AMAl-Dhubiab BEVenugopala KNDove Medical PressarticleNanoparticlesCandida Antarctica Lipase BgraftingimmobilizationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3235-3244 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanoparticles Candida Antarctica Lipase B grafting immobilization Medicine (General) R5-920
spellingShingle	Nanoparticles Candida Antarctica Lipase B grafting immobilization Medicine (General) R5-920 SreeHarsha N Ghorpade RV Alzahrani AM Al-Dhubiab BE Venugopala KN Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles
description	Nagaraja SreeHarsha,1 Ravindra V Ghorpade,2 Abdullah Mossa Alzahrani,3 Bandar E Al-Dhubiab,1 Katharigatta N Venugopala1,41Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, Saudi Arabia; 2Chemical Engineering and Process Development Division, CSIR-National Chemical Laboratory, Pune 411 008, India; 3Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia; 4Department of Biotechnology and Food Technology, Durban University of Technology, Durban 4001, South AfricaPurpose: Here, we present the successful preparation of a highly efficient gallic acid resin grafted with magnetic nanoparticles (MNPs) and containing a branched brush polymeric shell.Methods: Using a convenient co-precipitation method, we prepared Fe3O4 nanoparticles stabilized by citric acid. These nanoparticles underwent further silica modification and amino functionalization followed by gallic acid functionalization on their surface. Under alkaline conditions, we used a condensation reaction that combined formaldehyde and gallic, to graft the gallic acid−formaldehyde resin on the surface. We then evaluated the polymer-grafted MNPs to assay the Candida Antarctica B lipase(Cal-B) immobilization via physical adsorption.Conclusion: Furthermore, during optimization of parameters that defined conditions of immobilization, we found that the optimum immobilization was achieved in 15 mins. Also, optimal immobilization temperature and pH were 38ºC and 7.5, respectively. In addition, the reusability study of immobilized lipase polymer-grafted MNPs was done by isolating the MNPs from the reaction medium using magnetic separation, which showed that grafted MNPs reached 5 cycles with 91% activity retention.Keywords: nanoparticles, Candida Antarctica lipase B, grafting, immobilization
format	article
author	SreeHarsha N Ghorpade RV Alzahrani AM Al-Dhubiab BE Venugopala KN
author_facet	SreeHarsha N Ghorpade RV Alzahrani AM Al-Dhubiab BE Venugopala KN
author_sort	SreeHarsha N
title	Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles
title_short	Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles
title_full	Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles
title_fullStr	Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles
title_full_unstemmed	Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles
title_sort	immobilization studies of candida antarctica lipase b on gallic acid resin-grafted magnetic iron oxide nanoparticles
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/8656e96c9333486092f09de4a536a429
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Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles

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