Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings

Brown-marbled grouper Epinephelus fuscoguttatus (Forsskål) fingerlings are more vulnerable to diseases than the adult grouper because the fingerlings’ adaptive immune system is still in the development stage. The mortality rates are approximately 20 to 70% during outbreaks of vibriosis in aquacultur...

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Autores principales:	N Mohamed Alipiah, NHS Ramli, CF Low, MN Shamsudin, FM Yusoff
Formato:	article
Lenguaje:	EN
Publicado:	Inter-Research 2016
Materias:	Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/8659903b71e44aefaa6c3fdfc05611d8
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spelling	oai:doaj.org-article:8659903b71e44aefaa6c3fdfc05611d82021-11-04T10:29:29ZProtective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings1869-215X1869-753410.3354/aei00169https://doaj.org/article/8659903b71e44aefaa6c3fdfc05611d82016-02-01T00:00:00Zhttps://www.int-res.com/abstracts/aei/v8/p147-155/https://doaj.org/toc/1869-215Xhttps://doaj.org/toc/1869-7534Brown-marbled grouper Epinephelus fuscoguttatus (Forsskål) fingerlings are more vulnerable to diseases than the adult grouper because the fingerlings’ adaptive immune system is still in the development stage. The mortality rates are approximately 20 to 70% during outbreaks of vibriosis in aquacultures of grouper fingerlings. Studies were conducted to identify alternative treatments with low impacts on humans, animals and the environment as well as treatments that minimize the use of antibiotics in aquaculture. In this study, we report the first use of surface-associated bacteria isolated from marine invertebrates to increase fingerlings’ resistance against infections. Twenty-two surface-associated bacteria were isolated from the sea cucumber Stichopus badionotus and were identified with 16S rRNA gene sequencing. Three of the surface-associated bacteria had inhibitory activities against pathogenic Vibrio harveyi and V. parahaemolyticus. The fingerlings treated with the surface-associated bacteria Exiguobacterium acetylicum for 12 d prior to the challenge experiment with pathogenic V. harveyi showed significantly higher survival rates and increases in antibody titres compared to the control group. This study illustrats a symbiotic interaction between E. acetylicum and grouper fingerlings. E. acetylicum colonized the scales of grouper fingerlings and enhanced the fish’s immune response against the pathogen V. harveyi.N Mohamed AlipiahNHS RamliCF LowMN ShamsudinFM YusoffInter-ResearcharticleAquaculture. Fisheries. AnglingSH1-691EcologyQH540-549.5ENAquaculture Environment Interactions, Vol 8, Pp 147-155 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5
spellingShingle	Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 N Mohamed Alipiah NHS Ramli CF Low MN Shamsudin FM Yusoff Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings
description	Brown-marbled grouper Epinephelus fuscoguttatus (Forsskål) fingerlings are more vulnerable to diseases than the adult grouper because the fingerlings’ adaptive immune system is still in the development stage. The mortality rates are approximately 20 to 70% during outbreaks of vibriosis in aquacultures of grouper fingerlings. Studies were conducted to identify alternative treatments with low impacts on humans, animals and the environment as well as treatments that minimize the use of antibiotics in aquaculture. In this study, we report the first use of surface-associated bacteria isolated from marine invertebrates to increase fingerlings’ resistance against infections. Twenty-two surface-associated bacteria were isolated from the sea cucumber Stichopus badionotus and were identified with 16S rRNA gene sequencing. Three of the surface-associated bacteria had inhibitory activities against pathogenic Vibrio harveyi and V. parahaemolyticus. The fingerlings treated with the surface-associated bacteria Exiguobacterium acetylicum for 12 d prior to the challenge experiment with pathogenic V. harveyi showed significantly higher survival rates and increases in antibody titres compared to the control group. This study illustrats a symbiotic interaction between E. acetylicum and grouper fingerlings. E. acetylicum colonized the scales of grouper fingerlings and enhanced the fish’s immune response against the pathogen V. harveyi.
format	article
author	N Mohamed Alipiah NHS Ramli CF Low MN Shamsudin FM Yusoff
author_facet	N Mohamed Alipiah NHS Ramli CF Low MN Shamsudin FM Yusoff
author_sort	N Mohamed Alipiah
title	Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings
title_short	Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings
title_full	Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings
title_fullStr	Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings
title_full_unstemmed	Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings
title_sort	protective effects of sea cucumber surface-associated bacteria against vibrio harveyi in brown-marbled grouper fingerlings
publisher	Inter-Research
publishDate	2016
url	https://doaj.org/article/8659903b71e44aefaa6c3fdfc05611d8
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Protective effects of sea cucumber surface-associated bacteria against Vibrio harveyi in brown-marbled grouper fingerlings

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