Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene

Agung PP, Putra WPB, Anwar S, Wulandari AS, Zein MSA, Said S, Sudiro A. 2018. Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene. Biodiversitas 19: 676-681. The Sumba Ongole cattle (Bos indicus) is one of the local Indonesian cattle breeds and has excellent...

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Autores principales:	PASKAH PARTOGI AGUNG, WIDYA PINTAKA BAYU PUTRA, SAIFUL ANWAR, ARI SULISTYO WULANDARI, SYAHRUDDIN SAID, MOCH SYAMSUL ARIFIN ZEIN, ADITYA SUDIRO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	bos indicus growth hormone novel snp sumba ongole Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/86627f93acdb49209359e14ff44ed687
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spelling	oai:doaj.org-article:86627f93acdb49209359e14ff44ed6872021-11-16T14:01:41ZNovel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene1412-033X2085-472210.13057/biodiv/d190240https://doaj.org/article/86627f93acdb49209359e14ff44ed6872018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3029https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Agung PP, Putra WPB, Anwar S, Wulandari AS, Zein MSA, Said S, Sudiro A. 2018. Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene. Biodiversitas 19: 676-681. The Sumba Ongole cattle (Bos indicus) is one of the local Indonesian cattle breeds and has excellent potential to gain higher carcass yield compared to other local cattle breeds in Indonesia. The Growth Hormone (GH) gene was a potential target for molecular study due to its importance as growth regulating hormone. This study was aimed to determine Single Nucleotide Polymorphism (SNP) profile of the GH gene in the Sumba Ongole (SO) cattle using PCR and DNA sequencing methods from 34 individual DNA samples. The results from this study showed that there are 28 SNPs identified within the GH gene; Twenty one SNPs were found to occur in the intron region, 4 SNPs in the exon region, and 3 SNPs in the 3’ end region. There were 17 SNPs in the SO cattle GH gene were caused by novel mutation. The novel SNP g.1395insC was found only in the SO cattle. Moreover, the frequency of the SNP g.1395insC was the highest among other novel SNPs in the SO cattle GH gene. The moderate PIC value (0.25<PIC<0.50) were found in two novel SNPs i.e. SNP g.1415C>G (intron 4), and SNP g.1526A>G (intron 4). The GH gene in the SO cattle was polymorphic and the 17 novel SNPs need to be further validated.PASKAH PARTOGI AGUNGWIDYA PINTAKA BAYU PUTRASAIFUL ANWARARI SULISTYO WULANDARISYAHRUDDIN SAIDMOCH SYAMSUL ARIFIN ZEINADITYA SUDIROMBI & UNS Soloarticlebos indicusgrowth hormonenovel snpsumba ongoleBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 2, Pp 626-631 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	bos indicus growth hormone novel snp sumba ongole Biology (General) QH301-705.5
spellingShingle	bos indicus growth hormone novel snp sumba ongole Biology (General) QH301-705.5 PASKAH PARTOGI AGUNG WIDYA PINTAKA BAYU PUTRA SAIFUL ANWAR ARI SULISTYO WULANDARI SYAHRUDDIN SAID MOCH SYAMSUL ARIFIN ZEIN ADITYA SUDIRO Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene
description	Agung PP, Putra WPB, Anwar S, Wulandari AS, Zein MSA, Said S, Sudiro A. 2018. Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene. Biodiversitas 19: 676-681. The Sumba Ongole cattle (Bos indicus) is one of the local Indonesian cattle breeds and has excellent potential to gain higher carcass yield compared to other local cattle breeds in Indonesia. The Growth Hormone (GH) gene was a potential target for molecular study due to its importance as growth regulating hormone. This study was aimed to determine Single Nucleotide Polymorphism (SNP) profile of the GH gene in the Sumba Ongole (SO) cattle using PCR and DNA sequencing methods from 34 individual DNA samples. The results from this study showed that there are 28 SNPs identified within the GH gene; Twenty one SNPs were found to occur in the intron region, 4 SNPs in the exon region, and 3 SNPs in the 3’ end region. There were 17 SNPs in the SO cattle GH gene were caused by novel mutation. The novel SNP g.1395insC was found only in the SO cattle. Moreover, the frequency of the SNP g.1395insC was the highest among other novel SNPs in the SO cattle GH gene. The moderate PIC value (0.25<PIC<0.50) were found in two novel SNPs i.e. SNP g.1415C>G (intron 4), and SNP g.1526A>G (intron 4). The GH gene in the SO cattle was polymorphic and the 17 novel SNPs need to be further validated.
format	article
author	PASKAH PARTOGI AGUNG WIDYA PINTAKA BAYU PUTRA SAIFUL ANWAR ARI SULISTYO WULANDARI SYAHRUDDIN SAID MOCH SYAMSUL ARIFIN ZEIN ADITYA SUDIRO
author_facet	PASKAH PARTOGI AGUNG WIDYA PINTAKA BAYU PUTRA SAIFUL ANWAR ARI SULISTYO WULANDARI SYAHRUDDIN SAID MOCH SYAMSUL ARIFIN ZEIN ADITYA SUDIRO
author_sort	PASKAH PARTOGI AGUNG
title	Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene
title_short	Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene
title_full	Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene
title_fullStr	Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene
title_full_unstemmed	Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene
title_sort	novel single nucleotide polymorphisms in the sumba ongole (bos indicus) growth hormone gene
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/86627f93acdb49209359e14ff44ed687
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Novel Single Nucleotide Polymorphisms in the Sumba Ongole (Bos indicus) Growth Hormone Gene

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