Caffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice

Caffeic acid (CA) is one of the major phenolic acids of coffee with multiple biological activities. Our previous study found that 500 mg/kg of chlorogenic acid (CGA) had the potential capacity of alleviating colonic inflammation. Moreover, CGA can be degraded into caffeic acid (CA) by the gut microb...

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Autores principales:	Fan Wan, Ruqing Zhong, Mengyu Wang, Yexun Zhou, Yuxia Chen, Bao Yi, Fujiang Hou, Lei Liu, Yong Zhao, Liang Chen, Hongfu Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	caffeic acid colitis gut microbiota oxidative stress inflammatory responses Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8662cfaf02bc4fcca65cd243df7aca89
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spelling	oai:doaj.org-article:8662cfaf02bc4fcca65cd243df7aca892021-11-16T07:45:53ZCaffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice1664-302X10.3389/fmicb.2021.784211https://doaj.org/article/8662cfaf02bc4fcca65cd243df7aca892021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.784211/fullhttps://doaj.org/toc/1664-302XCaffeic acid (CA) is one of the major phenolic acids of coffee with multiple biological activities. Our previous study found that 500 mg/kg of chlorogenic acid (CGA) had the potential capacity of alleviating colonic inflammation. Moreover, CGA can be degraded into caffeic acid (CA) by the gut microbiota in the colon. Therefore, we hypothesize that CA can exert protective effects on colonic inflammation. To test the hypothesis, 251 mg/kg CA was supplemented to DSS-induced colitis mice. The results showed that CA treatment recovered DSS-induced disease activity index (DAI), colon length, and histopathology scores of colon tissue. Additionally, CA treatment significantly decreased pro-inflammatory cytokines and malondialdehyde (MDA) levels and increased the level of IL-10, total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in serum. qPCR results indicated that CA treatment dramatically downregulated mRNA expression of IL-1β, IL-6, and TNF-α as well as upregulated SOD1, GPX1, GPX2, CAT, and IL-10. In addition, CA supplementation significantly increased mRNA expression of Nrf-2, HO-1, and NQO1, which showed its antioxidant and anti-inflammatory capacities potentially by activating the Nrf-2/HO-1 pathway. Moreover, CA supplementation prevented gut barrier damage by enhancing Occludin gene expression. Furthermore, CA supplementation altered the gut microbiome composition by decreasing the relative abundance of Bacteroides and Turicibacter, and enhancing the relative abundance of Alistipes and Dubosiella. Meanwhile, CA supplementation increases the abundance of Dubosiella and Akkermansia. In conclusion, CA supplementation could effectively alleviate DSS-induced colitis by improving the defense against oxidative stress and inflammatory response.Fan WanFan WanRuqing ZhongMengyu WangYexun ZhouYuxia ChenBao YiFujiang HouLei LiuYong ZhaoLiang ChenHongfu ZhangFrontiers Media S.A.articlecaffeic acidcolitisgut microbiotaoxidative stressinflammatory responsesMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	caffeic acid colitis gut microbiota oxidative stress inflammatory responses Microbiology QR1-502
spellingShingle	caffeic acid colitis gut microbiota oxidative stress inflammatory responses Microbiology QR1-502 Fan Wan Fan Wan Ruqing Zhong Mengyu Wang Yexun Zhou Yuxia Chen Bao Yi Fujiang Hou Lei Liu Yong Zhao Liang Chen Hongfu Zhang Caffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice
description	Caffeic acid (CA) is one of the major phenolic acids of coffee with multiple biological activities. Our previous study found that 500 mg/kg of chlorogenic acid (CGA) had the potential capacity of alleviating colonic inflammation. Moreover, CGA can be degraded into caffeic acid (CA) by the gut microbiota in the colon. Therefore, we hypothesize that CA can exert protective effects on colonic inflammation. To test the hypothesis, 251 mg/kg CA was supplemented to DSS-induced colitis mice. The results showed that CA treatment recovered DSS-induced disease activity index (DAI), colon length, and histopathology scores of colon tissue. Additionally, CA treatment significantly decreased pro-inflammatory cytokines and malondialdehyde (MDA) levels and increased the level of IL-10, total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in serum. qPCR results indicated that CA treatment dramatically downregulated mRNA expression of IL-1β, IL-6, and TNF-α as well as upregulated SOD1, GPX1, GPX2, CAT, and IL-10. In addition, CA supplementation significantly increased mRNA expression of Nrf-2, HO-1, and NQO1, which showed its antioxidant and anti-inflammatory capacities potentially by activating the Nrf-2/HO-1 pathway. Moreover, CA supplementation prevented gut barrier damage by enhancing Occludin gene expression. Furthermore, CA supplementation altered the gut microbiome composition by decreasing the relative abundance of Bacteroides and Turicibacter, and enhancing the relative abundance of Alistipes and Dubosiella. Meanwhile, CA supplementation increases the abundance of Dubosiella and Akkermansia. In conclusion, CA supplementation could effectively alleviate DSS-induced colitis by improving the defense against oxidative stress and inflammatory response.
format	article
author	Fan Wan Fan Wan Ruqing Zhong Mengyu Wang Yexun Zhou Yuxia Chen Bao Yi Fujiang Hou Lei Liu Yong Zhao Liang Chen Hongfu Zhang
author_facet	Fan Wan Fan Wan Ruqing Zhong Mengyu Wang Yexun Zhou Yuxia Chen Bao Yi Fujiang Hou Lei Liu Yong Zhao Liang Chen Hongfu Zhang
author_sort	Fan Wan
title	Caffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice
title_short	Caffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice
title_full	Caffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice
title_fullStr	Caffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice
title_full_unstemmed	Caffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice
title_sort	caffeic acid supplement alleviates colonic inflammation and oxidative stress potentially through improved gut microbiota community in mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/8662cfaf02bc4fcca65cd243df7aca89
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Caffeic Acid Supplement Alleviates Colonic Inflammation and Oxidative Stress Potentially Through Improved Gut Microbiota Community in Mice

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