Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams

Abstract To clarify the health risks of internal radiation exposure, it is important to investigate the radiological effects of local exposure at cell levels from radioactive materials taken up by organs. Focusing on the response of cell populations post-irradiation, X-ray microbeams are very effect...

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Autores principales:	Mitsuaki Ojima, Atsushi Ito, Noriko Usami, Maki Ohara, Keiji Suzuki, Michiaki Kai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/866a903ab8eb4d4f9241f4880abee753
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spelling	oai:doaj.org-article:866a903ab8eb4d4f9241f4880abee7532021-12-02T17:04:06ZField size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams10.1038/s41598-021-86416-72045-2322https://doaj.org/article/866a903ab8eb4d4f9241f4880abee7532021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86416-7https://doaj.org/toc/2045-2322Abstract To clarify the health risks of internal radiation exposure, it is important to investigate the radiological effects of local exposure at cell levels from radioactive materials taken up by organs. Focusing on the response of cell populations post-irradiation, X-ray microbeams are very effective at reproducing the effects of local exposure within an internal exposure in vitro. The present study aims to clarify the effects of local exposure by investigating the response of normal human cell (MRC-5) populations irradiated with X-ray microbeams of different beam sizes to DNA damage. The populations of MRC-5 were locally irradiated with X-ray microbeams of 1 Gy at 0.02–1.89 mm2 field sizes, and analyzed whether the number of 53BP1 foci as DSB (DNA double strand break) per cell changed with the field size. We found that even at the same dose, the number of DSB per cell increased depending on the X-irradiated field size on the cell population. This result indicated that DNA damage repair of X-irradiated cells might be enhanced in small size fields surrounded by non-irradiated cells. This study suggests that X-irradiated cells received some signal (a rescue signal) from surrounding non-irradiated cells may be involved in the response of cell populations post-irradiation.Mitsuaki OjimaAtsushi ItoNoriko UsamiMaki OharaKeiji SuzukiMichiaki KaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mitsuaki Ojima Atsushi Ito Noriko Usami Maki Ohara Keiji Suzuki Michiaki Kai Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams
description	Abstract To clarify the health risks of internal radiation exposure, it is important to investigate the radiological effects of local exposure at cell levels from radioactive materials taken up by organs. Focusing on the response of cell populations post-irradiation, X-ray microbeams are very effective at reproducing the effects of local exposure within an internal exposure in vitro. The present study aims to clarify the effects of local exposure by investigating the response of normal human cell (MRC-5) populations irradiated with X-ray microbeams of different beam sizes to DNA damage. The populations of MRC-5 were locally irradiated with X-ray microbeams of 1 Gy at 0.02–1.89 mm2 field sizes, and analyzed whether the number of 53BP1 foci as DSB (DNA double strand break) per cell changed with the field size. We found that even at the same dose, the number of DSB per cell increased depending on the X-irradiated field size on the cell population. This result indicated that DNA damage repair of X-irradiated cells might be enhanced in small size fields surrounded by non-irradiated cells. This study suggests that X-irradiated cells received some signal (a rescue signal) from surrounding non-irradiated cells may be involved in the response of cell populations post-irradiation.
format	article
author	Mitsuaki Ojima Atsushi Ito Noriko Usami Maki Ohara Keiji Suzuki Michiaki Kai
author_facet	Mitsuaki Ojima Atsushi Ito Noriko Usami Maki Ohara Keiji Suzuki Michiaki Kai
author_sort	Mitsuaki Ojima
title	Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams
title_short	Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams
title_full	Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams
title_fullStr	Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams
title_full_unstemmed	Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams
title_sort	field size effects on dna damage and proliferation in normal human cell populations irradiated with x-ray microbeams
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/866a903ab8eb4d4f9241f4880abee753
work_keys_str_mv	AT mitsuakiojima fieldsizeeffectsondnadamageandproliferationinnormalhumancellpopulationsirradiatedwithxraymicrobeams AT atsushiito fieldsizeeffectsondnadamageandproliferationinnormalhumancellpopulationsirradiatedwithxraymicrobeams AT norikousami fieldsizeeffectsondnadamageandproliferationinnormalhumancellpopulationsirradiatedwithxraymicrobeams AT makiohara fieldsizeeffectsondnadamageandproliferationinnormalhumancellpopulationsirradiatedwithxraymicrobeams AT keijisuzuki fieldsizeeffectsondnadamageandproliferationinnormalhumancellpopulationsirradiatedwithxraymicrobeams AT michiakikai fieldsizeeffectsondnadamageandproliferationinnormalhumancellpopulationsirradiatedwithxraymicrobeams
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Field size effects on DNA damage and proliferation in normal human cell populations irradiated with X-ray microbeams

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