The Biochemical and Molecular Analysis of Changes in Melanogenesis Induced by UVA-Activated Fluoroquinolones—In Vitro Study on Human Normal Melanocytes
Fluoroquinolones cause phototoxic reactions, manifested as different types of skin lesions, including hyperpigmentation. The disturbances of melanogenesis indicate that fluoroquinolones may affect cellular processes in melanocytes. It has been reported that these antibiotics may bind with melanin an...
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oai:doaj.org-article:866fe8662c3c4858b2960cd8c61cc5ea2021-11-25T17:08:42ZThe Biochemical and Molecular Analysis of Changes in Melanogenesis Induced by UVA-Activated Fluoroquinolones—In Vitro Study on Human Normal Melanocytes10.3390/cells101129002073-4409https://doaj.org/article/866fe8662c3c4858b2960cd8c61cc5ea2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/2900https://doaj.org/toc/2073-4409Fluoroquinolones cause phototoxic reactions, manifested as different types of skin lesions, including hyperpigmentation. The disturbances of melanogenesis indicate that fluoroquinolones may affect cellular processes in melanocytes. It has been reported that these antibiotics may bind with melanin and accumulate in pigmented cells. The study aimed to examine the changes in melanogenesis in human normal melanocytes exposed to UVA radiation and treated with lomefloxacin and moxifloxacin, the most and the least fluoroquinolone, respectively. The obtained results demonstrated that both tested fluoroquinolones inhibited melanogenesis through a decrease in tyrosinase activity and down-regulation of tyrosinase and microphthalmia-associated transcription factor production. Only lomefloxacin potentiated UVA-induced melanogenesis. Under UVA irradiation lomefloxacin significantly enhanced melanin content and tyrosinase activity in melanocytes, although the drug did not cause an increased expression of tyrosinase or microphthalmia-associated transcription factor. The current studies revealed that phototoxic activity of fluoroquinolones is associated with alterations in the melanogenesis process. The difference in phototoxic potential of fluoroquinolones derivatives may be connected with various effects on UVA-induced events at a cellular level.Justyna KowalskaKlaudia BanachArtur BeberokJakub RokZuzanna RzepkaDorota WrześniokMDPI AGarticlemoxifloxacinlomefloxacinmelanintyrosinasemicrophthalmia-associated transcription factormelanogenesisBiology (General)QH301-705.5ENCells, Vol 10, Iss 2900, p 2900 (2021) |
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| collection |
DOAJ |
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EN |
| topic |
moxifloxacin lomefloxacin melanin tyrosinase microphthalmia-associated transcription factor melanogenesis Biology (General) QH301-705.5 |
| spellingShingle |
moxifloxacin lomefloxacin melanin tyrosinase microphthalmia-associated transcription factor melanogenesis Biology (General) QH301-705.5 Justyna Kowalska Klaudia Banach Artur Beberok Jakub Rok Zuzanna Rzepka Dorota Wrześniok The Biochemical and Molecular Analysis of Changes in Melanogenesis Induced by UVA-Activated Fluoroquinolones—In Vitro Study on Human Normal Melanocytes |
| description |
Fluoroquinolones cause phototoxic reactions, manifested as different types of skin lesions, including hyperpigmentation. The disturbances of melanogenesis indicate that fluoroquinolones may affect cellular processes in melanocytes. It has been reported that these antibiotics may bind with melanin and accumulate in pigmented cells. The study aimed to examine the changes in melanogenesis in human normal melanocytes exposed to UVA radiation and treated with lomefloxacin and moxifloxacin, the most and the least fluoroquinolone, respectively. The obtained results demonstrated that both tested fluoroquinolones inhibited melanogenesis through a decrease in tyrosinase activity and down-regulation of tyrosinase and microphthalmia-associated transcription factor production. Only lomefloxacin potentiated UVA-induced melanogenesis. Under UVA irradiation lomefloxacin significantly enhanced melanin content and tyrosinase activity in melanocytes, although the drug did not cause an increased expression of tyrosinase or microphthalmia-associated transcription factor. The current studies revealed that phototoxic activity of fluoroquinolones is associated with alterations in the melanogenesis process. The difference in phototoxic potential of fluoroquinolones derivatives may be connected with various effects on UVA-induced events at a cellular level. |
| format |
article |
| author |
Justyna Kowalska Klaudia Banach Artur Beberok Jakub Rok Zuzanna Rzepka Dorota Wrześniok |
| author_facet |
Justyna Kowalska Klaudia Banach Artur Beberok Jakub Rok Zuzanna Rzepka Dorota Wrześniok |
| author_sort |
Justyna Kowalska |
| title |
The Biochemical and Molecular Analysis of Changes in Melanogenesis Induced by UVA-Activated Fluoroquinolones—In Vitro Study on Human Normal Melanocytes |
| title_short |
The Biochemical and Molecular Analysis of Changes in Melanogenesis Induced by UVA-Activated Fluoroquinolones—In Vitro Study on Human Normal Melanocytes |
| title_full |
The Biochemical and Molecular Analysis of Changes in Melanogenesis Induced by UVA-Activated Fluoroquinolones—In Vitro Study on Human Normal Melanocytes |
| title_fullStr |
The Biochemical and Molecular Analysis of Changes in Melanogenesis Induced by UVA-Activated Fluoroquinolones—In Vitro Study on Human Normal Melanocytes |
| title_full_unstemmed |
The Biochemical and Molecular Analysis of Changes in Melanogenesis Induced by UVA-Activated Fluoroquinolones—In Vitro Study on Human Normal Melanocytes |
| title_sort |
biochemical and molecular analysis of changes in melanogenesis induced by uva-activated fluoroquinolones—in vitro study on human normal melanocytes |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/866fe8662c3c4858b2960cd8c61cc5ea |
| work_keys_str_mv |
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