Neurohypophysial Hormones Associated with Osmotic Challenges in the Brain and Pituitary of the Euryhaline Black Porgy, <i>Acanthopagrus schlegelii</i>
Our study showed differential expression of the <i>arginine vasotocin</i> (<i>avt</i>)/<i>isotocin</i> (<i>it</i>) in the brain and pituitary gland of the euryhaline black porgy (<i>Acanthopagrus schlegelii</i>) during osmotic stress. A dec...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/86758899254b4d5a9998a969416694f0 |
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| Sumario: | Our study showed differential expression of the <i>arginine vasotocin</i> (<i>avt</i>)/<i>isotocin</i> (<i>it</i>) in the brain and pituitary gland of the euryhaline black porgy (<i>Acanthopagrus schlegelii</i>) during osmotic stress. A decrease in serum osmolality and increased cortisol levels were observed after acute transfer from seawater (SW) to freshwater (FW). The increased expressions of <i>avt</i>, <i>avt receptor</i> (<i>avtr</i>: <i>v1a</i>), and <i>isotocin receptor</i> (<i>itr</i>: <i>itr1)</i> transcripts on day 1 and <i>it</i> and <i>itr</i> transcripts on days 7 and 30 were found in the brains and pituitary glands of FW fish. Increased levels of <i>avt</i> mRNA in the diencephalon and <i>avtr</i> mRNA in the pituitary together with serum cortisol on day 1 of FW exposure indicated activation of the hypothalamic–pituitary–interrenal (HPI) axis. The expression levels of <i>avtr</i> and <i>itr</i> after FW transfer were increased in the pituitary on days 7 and 30. Furthermore, <i>in situ</i> hybridization demonstrated spatially differential expression of <i>avt</i> and <i>itr</i> transcripts in nucleus preopticus parvocellularis of pars gigantocellularis (PMgc), magnocellularis (PMmc), and parvocellularis (PMpc) of the preoptic area (POA). Positive signals for <i>avt</i> and <i>it</i> were highly abundant in PMpc after FW exposure. The data suggest involvement of neurohypophysial hormones in the brain (telencephalon and diencephalon) and pituitary for osmotic stress. |
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