Expression of VEGFA-mRNA in classical and MSX2-mRNA in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis

Abstract Spondyloarthritis (SpA) is characterized by chronic inflammation and structural damage involving spine and peripheral joints. Monocytes, as part of innate immune system, following migration into affected tissue, may play a role in the pathogenesis of SpA. Here, potential associations betwee...

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Autores principales:	Małgorzata Stec, Michał Seweryn, Mariusz Korkosz, Zofia Guła, Rafał Szatanek, Kazimierz Węglarczyk, Magdalena Rutkowska-Zapała, Marzena Lenart, Marcin Czepiel, Jarosław Czyż, Jarosław Baran, Anna Gruca, Kamila Wojnar-Lasoń, Paweł Wołkow, Maciej Siedlar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/867ae2a3626743fc95376b2edc87c722
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spelling	oai:doaj.org-article:867ae2a3626743fc95376b2edc87c7222021-12-02T14:29:03ZExpression of VEGFA-mRNA in classical and MSX2-mRNA in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis10.1038/s41598-021-89037-22045-2322https://doaj.org/article/867ae2a3626743fc95376b2edc87c7222021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89037-2https://doaj.org/toc/2045-2322Abstract Spondyloarthritis (SpA) is characterized by chronic inflammation and structural damage involving spine and peripheral joints. Monocytes, as part of innate immune system, following migration into affected tissue, may play a role in the pathogenesis of SpA. Here, potential associations between osteogenesis-linked gene expression profile in particular monocyte subpopulations and clinical signs of SpA were investigated. The 20 patients with axial and 16 with peripheral SpA were enrolled in the study. Monocyte subpopulations (classical—CD14++CD16−, intermediate—CD14++CD16+ and non-classical—CD14+CD16++) were isolated from blood using flow cytometry and gene expression analysis was performed using real-time PCR method and TaqMan Array, Human Osteogenesis, Fast 96-well plates. Next, the characteristic clinical features shared by axial and peripheral SpA were analyzed in the context of the expression of selected genes in the three subpopulations of monocytes. We demonstrated that expression of VEGFA in classical and MSX2 in non-classical monocytes were associated with the number of swollen and painful peripheral joints of SpA patients. We conclude that monocytes may contribute to the development of peripheral arthritis in SpA patients. This might be possible through subpopulation specific effects, linking number of inflamed joints with expression of VEGFA in classical monocytes and MSX2 in non-classical monocytes.Małgorzata StecMichał SewerynMariusz KorkoszZofia GułaRafał SzatanekKazimierz WęglarczykMagdalena Rutkowska-ZapałaMarzena LenartMarcin CzepielJarosław CzyżJarosław BaranAnna GrucaKamila Wojnar-LasońPaweł WołkowMaciej SiedlarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Małgorzata Stec Michał Seweryn Mariusz Korkosz Zofia Guła Rafał Szatanek Kazimierz Węglarczyk Magdalena Rutkowska-Zapała Marzena Lenart Marcin Czepiel Jarosław Czyż Jarosław Baran Anna Gruca Kamila Wojnar-Lasoń Paweł Wołkow Maciej Siedlar Expression of VEGFA-mRNA in classical and MSX2-mRNA in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis
description	Abstract Spondyloarthritis (SpA) is characterized by chronic inflammation and structural damage involving spine and peripheral joints. Monocytes, as part of innate immune system, following migration into affected tissue, may play a role in the pathogenesis of SpA. Here, potential associations between osteogenesis-linked gene expression profile in particular monocyte subpopulations and clinical signs of SpA were investigated. The 20 patients with axial and 16 with peripheral SpA were enrolled in the study. Monocyte subpopulations (classical—CD14++CD16−, intermediate—CD14++CD16+ and non-classical—CD14+CD16++) were isolated from blood using flow cytometry and gene expression analysis was performed using real-time PCR method and TaqMan Array, Human Osteogenesis, Fast 96-well plates. Next, the characteristic clinical features shared by axial and peripheral SpA were analyzed in the context of the expression of selected genes in the three subpopulations of monocytes. We demonstrated that expression of VEGFA in classical and MSX2 in non-classical monocytes were associated with the number of swollen and painful peripheral joints of SpA patients. We conclude that monocytes may contribute to the development of peripheral arthritis in SpA patients. This might be possible through subpopulation specific effects, linking number of inflamed joints with expression of VEGFA in classical monocytes and MSX2 in non-classical monocytes.
format	article
author	Małgorzata Stec Michał Seweryn Mariusz Korkosz Zofia Guła Rafał Szatanek Kazimierz Węglarczyk Magdalena Rutkowska-Zapała Marzena Lenart Marcin Czepiel Jarosław Czyż Jarosław Baran Anna Gruca Kamila Wojnar-Lasoń Paweł Wołkow Maciej Siedlar
author_facet	Małgorzata Stec Michał Seweryn Mariusz Korkosz Zofia Guła Rafał Szatanek Kazimierz Węglarczyk Magdalena Rutkowska-Zapała Marzena Lenart Marcin Czepiel Jarosław Czyż Jarosław Baran Anna Gruca Kamila Wojnar-Lasoń Paweł Wołkow Maciej Siedlar
author_sort	Małgorzata Stec
title	Expression of VEGFA-mRNA in classical and MSX2-mRNA in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis
title_short	Expression of VEGFA-mRNA in classical and MSX2-mRNA in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis
title_full	Expression of VEGFA-mRNA in classical and MSX2-mRNA in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis
title_fullStr	Expression of VEGFA-mRNA in classical and MSX2-mRNA in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis
title_full_unstemmed	Expression of VEGFA-mRNA in classical and MSX2-mRNA in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis
title_sort	expression of vegfa-mrna in classical and msx2-mrna in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/867ae2a3626743fc95376b2edc87c722
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Expression of VEGFA-mRNA in classical and MSX2-mRNA in non-classical monocytes in patients with spondyloarthritis is associated with peripheral arthritis

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