Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer

An increasing number of studies have related the mitochondrial DNA (mtDNA) content to embryo viability and transfer outcomes. However, previous studies have focused more on the relationship between mtDNA and embryo implantation, few studies have studied the effect of the mtDNA content on live birth....

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Autores principales:	Xuanyou Zhou, Xueli Liu, Weihui Shi, Mujin Ye, Songchang Chen, Chenming Xu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	mitochondrial DNA live birth IVF embryo viability quiet embryo hypothesis Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/867af9060ae040fb807b737ab66088a9
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spelling	oai:doaj.org-article:867af9060ae040fb807b737ab66088a92021-11-16T16:00:48ZMitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer1664-239210.3389/fendo.2021.762976https://doaj.org/article/867af9060ae040fb807b737ab66088a92021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.762976/fullhttps://doaj.org/toc/1664-2392An increasing number of studies have related the mitochondrial DNA (mtDNA) content to embryo viability and transfer outcomes. However, previous studies have focused more on the relationship between mtDNA and embryo implantation, few studies have studied the effect of the mtDNA content on live birth. In the study, we investigated whether mtDNA content is a reliable screening biomarker for live birth after single blastocyst transfer. A total of 233 couples with 316 blastocyst stage embryos undergoing in vitro fertilization treatment and pre-implantation genetic testing analysis were included in the study. All embryos were chromosomally normal and had undergone single-embryo transfers. There was no significant difference observed in the blastocyst mtDNA content among the live birth, miscarriage and non-implanted groups (p=0.999), and the mtDNA content in blastocysts from the miscarriage and live birth groups was similar [median (interquartile range), 1.00108(7.59107- 1.39108) vs 1.01108 (7.37107- 1.32108)]. Similarly, no significant association was observed between mtDNA content and embryo implantation potential (p=0.965). After adjusting for multiple confounders in a logistic regression analysis with generalized estimating equations, no associations between mtDNA content and live birth were observed in all blastocysts, Day-5 and Day-6 blastocysts (p=0.567, p=0.673, p=0.165, respectively). The live birth rate was not significantly different between blastocysts with an elevated mtDNA content and blastocysts with a normal mtDNA content (26.7% vs 33.6% p=0.780). Additionally, there was no linear correlation between the mtDNA content and maternal age (p=0.570). In conclusion, the mtDNA content does not seem to be a potential biomarker for embryo transfer outcomes (i.e., implantation and live birth) based on the existing testing tools. Embryos with an elevated mtDNA content also have development potential for successful live birth.Xuanyou ZhouXuanyou ZhouXueli LiuXueli LiuWeihui ShiWeihui ShiMujin YeMujin YeSongchang ChenSongchang ChenSongchang ChenChenming XuChenming XuChenming XuFrontiers Media S.A.articlemitochondrial DNAlive birthIVFembryo viabilityquiet embryo hypothesisDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mitochondrial DNA live birth IVF embryo viability quiet embryo hypothesis Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	mitochondrial DNA live birth IVF embryo viability quiet embryo hypothesis Diseases of the endocrine glands. Clinical endocrinology RC648-665 Xuanyou Zhou Xuanyou Zhou Xueli Liu Xueli Liu Weihui Shi Weihui Shi Mujin Ye Mujin Ye Songchang Chen Songchang Chen Songchang Chen Chenming Xu Chenming Xu Chenming Xu Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer
description	An increasing number of studies have related the mitochondrial DNA (mtDNA) content to embryo viability and transfer outcomes. However, previous studies have focused more on the relationship between mtDNA and embryo implantation, few studies have studied the effect of the mtDNA content on live birth. In the study, we investigated whether mtDNA content is a reliable screening biomarker for live birth after single blastocyst transfer. A total of 233 couples with 316 blastocyst stage embryos undergoing in vitro fertilization treatment and pre-implantation genetic testing analysis were included in the study. All embryos were chromosomally normal and had undergone single-embryo transfers. There was no significant difference observed in the blastocyst mtDNA content among the live birth, miscarriage and non-implanted groups (p=0.999), and the mtDNA content in blastocysts from the miscarriage and live birth groups was similar [median (interquartile range), 1.00108(7.59107- 1.39108) vs 1.01108 (7.37107- 1.32108)]. Similarly, no significant association was observed between mtDNA content and embryo implantation potential (p=0.965). After adjusting for multiple confounders in a logistic regression analysis with generalized estimating equations, no associations between mtDNA content and live birth were observed in all blastocysts, Day-5 and Day-6 blastocysts (p=0.567, p=0.673, p=0.165, respectively). The live birth rate was not significantly different between blastocysts with an elevated mtDNA content and blastocysts with a normal mtDNA content (26.7% vs 33.6% p=0.780). Additionally, there was no linear correlation between the mtDNA content and maternal age (p=0.570). In conclusion, the mtDNA content does not seem to be a potential biomarker for embryo transfer outcomes (i.e., implantation and live birth) based on the existing testing tools. Embryos with an elevated mtDNA content also have development potential for successful live birth.
format	article
author	Xuanyou Zhou Xuanyou Zhou Xueli Liu Xueli Liu Weihui Shi Weihui Shi Mujin Ye Mujin Ye Songchang Chen Songchang Chen Songchang Chen Chenming Xu Chenming Xu Chenming Xu
author_facet	Xuanyou Zhou Xuanyou Zhou Xueli Liu Xueli Liu Weihui Shi Weihui Shi Mujin Ye Mujin Ye Songchang Chen Songchang Chen Songchang Chen Chenming Xu Chenming Xu Chenming Xu
author_sort	Xuanyou Zhou
title	Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer
title_short	Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer
title_full	Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer
title_fullStr	Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer
title_full_unstemmed	Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer
title_sort	mitochondrial dna content may not be a reliable screening biomarker for live birth after single euploid blastocyst transfer
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/867af9060ae040fb807b737ab66088a9
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Mitochondrial DNA Content May Not Be a Reliable Screening Biomarker for Live Birth After Single Euploid Blastocyst Transfer

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