Chromosomal integration of aldo-keto-reductase and short-chain dehydrogenase/reductase genes in Clostridium beijerinckii NCIMB 8052 enhanced tolerance to lignocellulose-derived microbial inhibitory compounds

Abstract In situ detoxification of lignocellulose-derived microbial inhibitory compounds is an economical strategy for the fermentation of lignocellulose-derived sugars to fuels and chemicals. In this study, we investigated homologous integration and constitutive expression of Cbei_3974 and Cbei_390...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Christopher Chukwudi Okonkwo, Victor Ujor, Thaddeus Chukwuemeka Ezeji
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
Materias:
R
Q
Acceso en línea:https://doaj.org/article/867b12958b2948f6926f0d85cee862b3
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:867b12958b2948f6926f0d85cee862b3
record_format dspace
spelling oai:doaj.org-article:867b12958b2948f6926f0d85cee862b32021-12-02T15:09:20ZChromosomal integration of aldo-keto-reductase and short-chain dehydrogenase/reductase genes in Clostridium beijerinckii NCIMB 8052 enhanced tolerance to lignocellulose-derived microbial inhibitory compounds10.1038/s41598-019-44061-12045-2322https://doaj.org/article/867b12958b2948f6926f0d85cee862b32019-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44061-1https://doaj.org/toc/2045-2322Abstract In situ detoxification of lignocellulose-derived microbial inhibitory compounds is an economical strategy for the fermentation of lignocellulose-derived sugars to fuels and chemicals. In this study, we investigated homologous integration and constitutive expression of Cbei_3974 and Cbei_3904, which encode aldo-keto reductase and previously annotated short chain dehydrogenase/reductase, respectively, in Clostridium beijerinckii NCIMB 8052 (Cb), resulting in two strains: Cb_3974 and Cb_3904. Expression of Cbei_3974 led to 2-fold increase in furfural detoxification relative to Cb_3904 and Cb_wild type. Correspondingly, butanol production was up to 1.2-fold greater in furfural-challenged cultures of Cb_3974 relative to Cb_3904 and Cb_wild type. With 4-hydroxybezaldehyde and syringaldehyde supplementation, Cb_3974 showed up to 2.4-fold increase in butanol concentration when compared to Cb_3904 and Cb_wild type. Syringic and vanillic acids were considerably less deleterious to all three strains of Cb tested. Overall, Cb_3974 showed greater tolerance to furfural, 4-hydroxybezaldehyde, and syringaldehyde with improved capacity for butanol production. Hence, development of Cb_3974 represents a significant progress towards engineering solventogenic Clostridium species that are tolerant to lignocellulosic biomass hydrolysates as substrates for ABE fermentation.Christopher Chukwudi OkonkwoVictor UjorThaddeus Chukwuemeka EzejiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-18 (2019)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Christopher Chukwudi Okonkwo
Victor Ujor
Thaddeus Chukwuemeka Ezeji
Chromosomal integration of aldo-keto-reductase and short-chain dehydrogenase/reductase genes in Clostridium beijerinckii NCIMB 8052 enhanced tolerance to lignocellulose-derived microbial inhibitory compounds
description Abstract In situ detoxification of lignocellulose-derived microbial inhibitory compounds is an economical strategy for the fermentation of lignocellulose-derived sugars to fuels and chemicals. In this study, we investigated homologous integration and constitutive expression of Cbei_3974 and Cbei_3904, which encode aldo-keto reductase and previously annotated short chain dehydrogenase/reductase, respectively, in Clostridium beijerinckii NCIMB 8052 (Cb), resulting in two strains: Cb_3974 and Cb_3904. Expression of Cbei_3974 led to 2-fold increase in furfural detoxification relative to Cb_3904 and Cb_wild type. Correspondingly, butanol production was up to 1.2-fold greater in furfural-challenged cultures of Cb_3974 relative to Cb_3904 and Cb_wild type. With 4-hydroxybezaldehyde and syringaldehyde supplementation, Cb_3974 showed up to 2.4-fold increase in butanol concentration when compared to Cb_3904 and Cb_wild type. Syringic and vanillic acids were considerably less deleterious to all three strains of Cb tested. Overall, Cb_3974 showed greater tolerance to furfural, 4-hydroxybezaldehyde, and syringaldehyde with improved capacity for butanol production. Hence, development of Cb_3974 represents a significant progress towards engineering solventogenic Clostridium species that are tolerant to lignocellulosic biomass hydrolysates as substrates for ABE fermentation.
format article
author Christopher Chukwudi Okonkwo
Victor Ujor
Thaddeus Chukwuemeka Ezeji
author_facet Christopher Chukwudi Okonkwo
Victor Ujor
Thaddeus Chukwuemeka Ezeji
author_sort Christopher Chukwudi Okonkwo
title Chromosomal integration of aldo-keto-reductase and short-chain dehydrogenase/reductase genes in Clostridium beijerinckii NCIMB 8052 enhanced tolerance to lignocellulose-derived microbial inhibitory compounds
title_short Chromosomal integration of aldo-keto-reductase and short-chain dehydrogenase/reductase genes in Clostridium beijerinckii NCIMB 8052 enhanced tolerance to lignocellulose-derived microbial inhibitory compounds
title_full Chromosomal integration of aldo-keto-reductase and short-chain dehydrogenase/reductase genes in Clostridium beijerinckii NCIMB 8052 enhanced tolerance to lignocellulose-derived microbial inhibitory compounds
title_fullStr Chromosomal integration of aldo-keto-reductase and short-chain dehydrogenase/reductase genes in Clostridium beijerinckii NCIMB 8052 enhanced tolerance to lignocellulose-derived microbial inhibitory compounds
title_full_unstemmed Chromosomal integration of aldo-keto-reductase and short-chain dehydrogenase/reductase genes in Clostridium beijerinckii NCIMB 8052 enhanced tolerance to lignocellulose-derived microbial inhibitory compounds
title_sort chromosomal integration of aldo-keto-reductase and short-chain dehydrogenase/reductase genes in clostridium beijerinckii ncimb 8052 enhanced tolerance to lignocellulose-derived microbial inhibitory compounds
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/867b12958b2948f6926f0d85cee862b3
work_keys_str_mv AT christopherchukwudiokonkwo chromosomalintegrationofaldoketoreductaseandshortchaindehydrogenasereductasegenesinclostridiumbeijerinckiincimb8052enhancedtolerancetolignocellulosederivedmicrobialinhibitorycompounds
AT victorujor chromosomalintegrationofaldoketoreductaseandshortchaindehydrogenasereductasegenesinclostridiumbeijerinckiincimb8052enhancedtolerancetolignocellulosederivedmicrobialinhibitorycompounds
AT thaddeuschukwuemekaezeji chromosomalintegrationofaldoketoreductaseandshortchaindehydrogenasereductasegenesinclostridiumbeijerinckiincimb8052enhancedtolerancetolignocellulosederivedmicrobialinhibitorycompounds
_version_ 1718387826078253056