Terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities

Abstract Over the past few years, biogenic methods for designing silver nanocomposites are in limelight due to their ability to generate semi-healthcare and para-pharmaceutical consumer goods. The present study reports the eco-friendly synthesis of silver nanoparticles from the hitherto unexplored m...

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Autores principales:	Pramod C. Mane, Shabnam A. R. Sayyed, Deepali D. Kadam, Manish D.Shinde, Amanullah Fatehmulla, Abdullah M. Aldhafiri, Eman A. Alghamdi, Dinesh P. Amalnerkar, Ravindra D. Chaudhari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/867fac8a5c7d48bca34b047cf4cac209
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spelling	oai:doaj.org-article:867fac8a5c7d48bca34b047cf4cac2092021-12-02T16:07:03ZTerrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities10.1038/s41598-021-92478-42045-2322https://doaj.org/article/867fac8a5c7d48bca34b047cf4cac2092021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92478-4https://doaj.org/toc/2045-2322Abstract Over the past few years, biogenic methods for designing silver nanocomposites are in limelight due to their ability to generate semi-healthcare and para-pharmaceutical consumer goods. The present study reports the eco-friendly synthesis of silver nanoparticles from the hitherto unexplored mucus of territorial snail Achatina fulica by the facile, clean and easily scalable method. The detailed characterization of the resultant samples by UV–Visible Spectroscopy, FESEM-EDS, XRD and FTIR Spectroscopy techniques corroborated the formation of silver nanoparticles in snail mucus matrix. The resultant samples were tested against a broad range of Gram positive and Gram negative bacteria like Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and a fungal strain Aspergillus fumigatus by well diffusion method. The results indicate that silver nanoparticles in mucus matrix exhibit strong antibacterial as well as antifungal activity. The pertinent experiments were also performed to determine the inhibitory concentration against both bacterial and fungal strains. Anticancer activity was executed by in vitro method using cervical cancer cell lines. Curiously, our biogenically synthesized Ag nanoparticles in biocompatible mucus revealed anticancer activity and demonstrated more than 15% inhibition of Hela cells. We suggest an interesting possibility of formulating antimicrobial and possibly anticancer creams/gels for topical applications in skin ailments.Pramod C. ManeShabnam A. R. SayyedDeepali D. KadamManish D.ShindeAmanullah FatehmullaAbdullah M. AldhafiriEman A. AlghamdiDinesh P. AmalnerkarRavindra D. ChaudhariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pramod C. Mane Shabnam A. R. Sayyed Deepali D. Kadam Manish D.Shinde Amanullah Fatehmulla Abdullah M. Aldhafiri Eman A. Alghamdi Dinesh P. Amalnerkar Ravindra D. Chaudhari Terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities
description	Abstract Over the past few years, biogenic methods for designing silver nanocomposites are in limelight due to their ability to generate semi-healthcare and para-pharmaceutical consumer goods. The present study reports the eco-friendly synthesis of silver nanoparticles from the hitherto unexplored mucus of territorial snail Achatina fulica by the facile, clean and easily scalable method. The detailed characterization of the resultant samples by UV–Visible Spectroscopy, FESEM-EDS, XRD and FTIR Spectroscopy techniques corroborated the formation of silver nanoparticles in snail mucus matrix. The resultant samples were tested against a broad range of Gram positive and Gram negative bacteria like Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and a fungal strain Aspergillus fumigatus by well diffusion method. The results indicate that silver nanoparticles in mucus matrix exhibit strong antibacterial as well as antifungal activity. The pertinent experiments were also performed to determine the inhibitory concentration against both bacterial and fungal strains. Anticancer activity was executed by in vitro method using cervical cancer cell lines. Curiously, our biogenically synthesized Ag nanoparticles in biocompatible mucus revealed anticancer activity and demonstrated more than 15% inhibition of Hela cells. We suggest an interesting possibility of formulating antimicrobial and possibly anticancer creams/gels for topical applications in skin ailments.
format	article
author	Pramod C. Mane Shabnam A. R. Sayyed Deepali D. Kadam Manish D.Shinde Amanullah Fatehmulla Abdullah M. Aldhafiri Eman A. Alghamdi Dinesh P. Amalnerkar Ravindra D. Chaudhari
author_facet	Pramod C. Mane Shabnam A. R. Sayyed Deepali D. Kadam Manish D.Shinde Amanullah Fatehmulla Abdullah M. Aldhafiri Eman A. Alghamdi Dinesh P. Amalnerkar Ravindra D. Chaudhari
author_sort	Pramod C. Mane
title	Terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities
title_short	Terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities
title_full	Terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities
title_fullStr	Terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities
title_full_unstemmed	Terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities
title_sort	terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/867fac8a5c7d48bca34b047cf4cac209
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Terrestrial snail-mucus mediated green synthesis of silver nanoparticles and in vitro investigations on their antimicrobial and anticancer activities

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