Expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.

<h4>Background</h4>Muscle satellite cells (MSCs) represent a devoted stem cell population that is responsible for postnatal muscle growth and skeletal muscle regeneration. An important characteristic of MSCs is that they encompass multi potential mesenchymal stem cell activity and are ab...

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Autores principales:	Eun Ju Lee, Majid Rasool Kamli, Smritee Pokharel, Adeel Malik, K M A Tareq, Abdul Roouf Bhat, Hee-Bok Park, Yong Seok Lee, SangHoon Kim, Bohsuk Yang, Ki Young Chung, Inho Choi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8686c63ab73f47c68f13e1ec77720d0b
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spelling	oai:doaj.org-article:8686c63ab73f47c68f13e1ec77720d0b2021-11-18T08:48:15ZExpressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.1932-620310.1371/journal.pone.0079780https://doaj.org/article/8686c63ab73f47c68f13e1ec77720d0b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24224006/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Muscle satellite cells (MSCs) represent a devoted stem cell population that is responsible for postnatal muscle growth and skeletal muscle regeneration. An important characteristic of MSCs is that they encompass multi potential mesenchymal stem cell activity and are able to differentiate into myocytes and adipocytes. To achieve a global view of the genes differentially expressed in MSCs, myotube formed-cells (MFCs) and adipocyte-like cells (ALCs), we performed large-scale EST sequencing of normalized cDNA libraries developed from bovine MSCs.<h4>Results</h4>A total of 24,192 clones were assembled into 3,333 clusters, 5,517 singletons and 3,842contigs. Functional annotation of these unigenes revealed that a large portion of the differentially expressed genes are involved in cellular and signaling processes. Database for Annotation, Visualization and Integrated Discovery (DAVID) functional analysis of three subsets of highly expressed gene lists (MSC233, MFC258, and ALC248) highlighted some common and unique biological processes among MSC, MFC and ALC. Additionally, genes that may be specific to MSC, MFC and ALC are reported here, and the role of dimethylarginine dimethylaminohydrolase2 (DDAH2) during myogenesis and hemoglobin subunit alpha2 (HBA2) during transdifferentiation in C2C12 were assayed as a case study. DDAH2 was up-regulated during myognesis and knockdown of DDAH2 by siRNA significantly decreased myogenin (MYOG) expression corresponding with the slight change in cell morphology. In contrast, HBA2 was up-regulated during ALC formation and resulted in decreased intracellular lipid accumulation and CD36 mRNA expression upon knockdown assay.<h4>Conclusion</h4>In this study, a large number of EST sequences were generated from the MSC, MFC and ALC. Overall, the collection of ESTs generated in this study provides a starting point for the identification of novel genes involved in MFC and ALC formation, which in turn offers a fundamental resource to enable better understanding of the mechanism of muscle differentiation and transdifferentiation.Eun Ju LeeMajid Rasool KamliSmritee PokharelAdeel MalikK M A TareqAbdul Roouf BhatHee-Bok ParkYong Seok LeeSangHoon KimBohsuk YangKi Young ChungInho ChoiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79780 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eun Ju Lee Majid Rasool Kamli Smritee Pokharel Adeel Malik K M A Tareq Abdul Roouf Bhat Hee-Bok Park Yong Seok Lee SangHoon Kim Bohsuk Yang Ki Young Chung Inho Choi Expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.
description	<h4>Background</h4>Muscle satellite cells (MSCs) represent a devoted stem cell population that is responsible for postnatal muscle growth and skeletal muscle regeneration. An important characteristic of MSCs is that they encompass multi potential mesenchymal stem cell activity and are able to differentiate into myocytes and adipocytes. To achieve a global view of the genes differentially expressed in MSCs, myotube formed-cells (MFCs) and adipocyte-like cells (ALCs), we performed large-scale EST sequencing of normalized cDNA libraries developed from bovine MSCs.<h4>Results</h4>A total of 24,192 clones were assembled into 3,333 clusters, 5,517 singletons and 3,842contigs. Functional annotation of these unigenes revealed that a large portion of the differentially expressed genes are involved in cellular and signaling processes. Database for Annotation, Visualization and Integrated Discovery (DAVID) functional analysis of three subsets of highly expressed gene lists (MSC233, MFC258, and ALC248) highlighted some common and unique biological processes among MSC, MFC and ALC. Additionally, genes that may be specific to MSC, MFC and ALC are reported here, and the role of dimethylarginine dimethylaminohydrolase2 (DDAH2) during myogenesis and hemoglobin subunit alpha2 (HBA2) during transdifferentiation in C2C12 were assayed as a case study. DDAH2 was up-regulated during myognesis and knockdown of DDAH2 by siRNA significantly decreased myogenin (MYOG) expression corresponding with the slight change in cell morphology. In contrast, HBA2 was up-regulated during ALC formation and resulted in decreased intracellular lipid accumulation and CD36 mRNA expression upon knockdown assay.<h4>Conclusion</h4>In this study, a large number of EST sequences were generated from the MSC, MFC and ALC. Overall, the collection of ESTs generated in this study provides a starting point for the identification of novel genes involved in MFC and ALC formation, which in turn offers a fundamental resource to enable better understanding of the mechanism of muscle differentiation and transdifferentiation.
format	article
author	Eun Ju Lee Majid Rasool Kamli Smritee Pokharel Adeel Malik K M A Tareq Abdul Roouf Bhat Hee-Bok Park Yong Seok Lee SangHoon Kim Bohsuk Yang Ki Young Chung Inho Choi
author_facet	Eun Ju Lee Majid Rasool Kamli Smritee Pokharel Adeel Malik K M A Tareq Abdul Roouf Bhat Hee-Bok Park Yong Seok Lee SangHoon Kim Bohsuk Yang Ki Young Chung Inho Choi
author_sort	Eun Ju Lee
title	Expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.
title_short	Expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.
title_full	Expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.
title_fullStr	Expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.
title_full_unstemmed	Expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.
title_sort	expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8686c63ab73f47c68f13e1ec77720d0b
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Expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.

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