Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns

Abstract Reducing the variability in nuclear transfer outcome requires a better understanding of its cellular and epigenetic determinants, in order to ensure safer fish regeneration from cryobanked somatic material. In this work, clones from goldfish were obtained using cryopreserved fin cells as do...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alexandra Depincé, Pierre-Yves Le Bail, Charlène Rouillon, Catherine Labbé
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/868991bcdab14a338024cf2358c00825
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:868991bcdab14a338024cf2358c00825
record_format	dspace
spelling	oai:doaj.org-article:868991bcdab14a338024cf2358c008252021-12-02T14:21:58ZEmbryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns10.1038/s41598-021-83033-22045-2322https://doaj.org/article/868991bcdab14a338024cf2358c008252021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83033-2https://doaj.org/toc/2045-2322Abstract Reducing the variability in nuclear transfer outcome requires a better understanding of its cellular and epigenetic determinants, in order to ensure safer fish regeneration from cryobanked somatic material. In this work, clones from goldfish were obtained using cryopreserved fin cells as donor and non-enucleated oocytes as recipients. We showed that the high variability of clones survival was not correlated to spawn quality. Clones were then characterized for their first cleavages pattern in relation to their developmental fate up to hatching. The first cell cycle duration was increased in clones with abnormal first cleavage, and symmetric first two cleavages increased clone probability to reach later on 24 h- and hatching-stages. At 24 h-stage, 24% of the clones were diploids and from donor genetic origin only. However, ploidy and genetic origin did not determine clones morphological quality. DNA methylation reprogramming in the promoter region of pou2, nanog, and notail marker genes was highly variable, but clones with the nicest morphologies displayed the best DNA methylation reprogramming. To conclude, non-enucleated oocytes did allow authentic clones production. The first two cell cycles were a critical determinant of the clone ability to reach hatching-stage, and DNA methylation reprogramming significantly influenced clones morphological quality.Alexandra DepincéPierre-Yves Le BailCharlène RouillonCatherine LabbéNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandra Depincé Pierre-Yves Le Bail Charlène Rouillon Catherine Labbé Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns
description	Abstract Reducing the variability in nuclear transfer outcome requires a better understanding of its cellular and epigenetic determinants, in order to ensure safer fish regeneration from cryobanked somatic material. In this work, clones from goldfish were obtained using cryopreserved fin cells as donor and non-enucleated oocytes as recipients. We showed that the high variability of clones survival was not correlated to spawn quality. Clones were then characterized for their first cleavages pattern in relation to their developmental fate up to hatching. The first cell cycle duration was increased in clones with abnormal first cleavage, and symmetric first two cleavages increased clone probability to reach later on 24 h- and hatching-stages. At 24 h-stage, 24% of the clones were diploids and from donor genetic origin only. However, ploidy and genetic origin did not determine clones morphological quality. DNA methylation reprogramming in the promoter region of pou2, nanog, and notail marker genes was highly variable, but clones with the nicest morphologies displayed the best DNA methylation reprogramming. To conclude, non-enucleated oocytes did allow authentic clones production. The first two cell cycles were a critical determinant of the clone ability to reach hatching-stage, and DNA methylation reprogramming significantly influenced clones morphological quality.
format	article
author	Alexandra Depincé Pierre-Yves Le Bail Charlène Rouillon Catherine Labbé
author_facet	Alexandra Depincé Pierre-Yves Le Bail Charlène Rouillon Catherine Labbé
author_sort	Alexandra Depincé
title	Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns
title_short	Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns
title_full	Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns
title_fullStr	Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns
title_full_unstemmed	Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns
title_sort	embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and dna methylation patterns
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/868991bcdab14a338024cf2358c00825
work_keys_str_mv	AT alexandradepince embryonicfateaftersomaticcellnucleartransferinnonenucleatedgoldfishoocytesisdeterminedbyfirstcleavagesanddnamethylationpatterns AT pierreyveslebail embryonicfateaftersomaticcellnucleartransferinnonenucleatedgoldfishoocytesisdeterminedbyfirstcleavagesanddnamethylationpatterns AT charlenerouillon embryonicfateaftersomaticcellnucleartransferinnonenucleatedgoldfishoocytesisdeterminedbyfirstcleavagesanddnamethylationpatterns AT catherinelabbe embryonicfateaftersomaticcellnucleartransferinnonenucleatedgoldfishoocytesisdeterminedbyfirstcleavagesanddnamethylationpatterns
_version_	1718391498589863936

Embryonic fate after somatic cell nuclear transfer in non-enucleated goldfish oocytes is determined by first cleavages and DNA methylation patterns

Ejemplares similares