Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model

Abstract Although the physiological function of the omentum remains elusive, it has been proposed that it plays an important role in fat storage, immune regulation, and regeneration of injured tissues and organs. Although the omentum undergoes expansion upon activation, reports on the accurate asses...

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Autores principales:	Kazuhiko Ishigaki, Keiki Kumano, Kyohei Fujita, Hiroo Ueno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/868aa4ac8f0642d9912e9ac70095290c
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spelling	oai:doaj.org-article:868aa4ac8f0642d9912e9ac70095290c2021-12-02T16:14:46ZCellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model10.1038/s41598-021-93330-52045-2322https://doaj.org/article/868aa4ac8f0642d9912e9ac70095290c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93330-5https://doaj.org/toc/2045-2322Abstract Although the physiological function of the omentum remains elusive, it has been proposed that it plays an important role in fat storage, immune regulation, and regeneration of injured tissues and organs. Although the omentum undergoes expansion upon activation, reports on the accurate assessment of increased cell types and the origin of the increased cells remain limited. To investigate this aspect, the omenta of parabiotic mice were subjected to activation using distinct fluorescent markers and single-cell RNA sequencing (scRNA-seq) was performed to identify circulation-derived omental cells. We found that a considerable number of circulating cells contributed to the activation of the omentum. The omental cells derived from circulating cells exhibited morphological features similar to those of fibroblasts. scRNA-seq revealed the existence of a novel cell population that co-expressed macrophage and fibroblast markers in the activated omentum, suggesting that it corresponded to circulating macrophage-derived fibroblast-like cells. Lineage tracing experiments revealed that most fibroblasts in the activated omentum were not derived from WT1-positive mesenchymal progenitors. The cell cluster also expressed various chemokine genes, indicating its role in the activation and recruitment of immune cells. These results provide important information regarding the activation of the omentum.Kazuhiko IshigakiKeiki KumanoKyohei FujitaHiroo UenoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazuhiko Ishigaki Keiki Kumano Kyohei Fujita Hiroo Ueno Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model
description	Abstract Although the physiological function of the omentum remains elusive, it has been proposed that it plays an important role in fat storage, immune regulation, and regeneration of injured tissues and organs. Although the omentum undergoes expansion upon activation, reports on the accurate assessment of increased cell types and the origin of the increased cells remain limited. To investigate this aspect, the omenta of parabiotic mice were subjected to activation using distinct fluorescent markers and single-cell RNA sequencing (scRNA-seq) was performed to identify circulation-derived omental cells. We found that a considerable number of circulating cells contributed to the activation of the omentum. The omental cells derived from circulating cells exhibited morphological features similar to those of fibroblasts. scRNA-seq revealed the existence of a novel cell population that co-expressed macrophage and fibroblast markers in the activated omentum, suggesting that it corresponded to circulating macrophage-derived fibroblast-like cells. Lineage tracing experiments revealed that most fibroblasts in the activated omentum were not derived from WT1-positive mesenchymal progenitors. The cell cluster also expressed various chemokine genes, indicating its role in the activation and recruitment of immune cells. These results provide important information regarding the activation of the omentum.
format	article
author	Kazuhiko Ishigaki Keiki Kumano Kyohei Fujita Hiroo Ueno
author_facet	Kazuhiko Ishigaki Keiki Kumano Kyohei Fujita Hiroo Ueno
author_sort	Kazuhiko Ishigaki
title	Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model
title_short	Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model
title_full	Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model
title_fullStr	Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model
title_full_unstemmed	Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model
title_sort	cellular basis of omentum activation and expansion revealed by single-cell rna sequencing using a parabiosis model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/868aa4ac8f0642d9912e9ac70095290c
work_keys_str_mv	AT kazuhikoishigaki cellularbasisofomentumactivationandexpansionrevealedbysinglecellrnasequencingusingaparabiosismodel AT keikikumano cellularbasisofomentumactivationandexpansionrevealedbysinglecellrnasequencingusingaparabiosismodel AT kyoheifujita cellularbasisofomentumactivationandexpansionrevealedbysinglecellrnasequencingusingaparabiosismodel AT hirooueno cellularbasisofomentumactivationandexpansionrevealedbysinglecellrnasequencingusingaparabiosismodel
_version_	1718384301848920064

Cellular basis of omentum activation and expansion revealed by single-cell RNA sequencing using a parabiosis model

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