Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm

Abstract Stem rust (caused by Puccinia graminis f. sp. tritici) is a major disease of wheat. To understand the genetic basis of stem rust resistance in Nebraska winter wheat, a set of 330 genotypes representing two nurseries (DUP2015 and TRP2015) were evaluated for resistance to a Nebraska stem rust...

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Autores principales:	Amira M. I. Mourad, Ahmed Sallam, Vikas Belamkar, Stephen Wegulo, Guihua Bai, Ezzat Mahdy, Bahy Bakheit, Atif Abo El-Wafa, Yue Jin, P. Stephen Baenziger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8699a88b10cf47c280e21c6b1b7280bb
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spelling	oai:doaj.org-article:8699a88b10cf47c280e21c6b1b7280bb2021-12-02T16:07:53ZMolecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm10.1038/s41598-019-47986-92045-2322https://doaj.org/article/8699a88b10cf47c280e21c6b1b7280bb2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47986-9https://doaj.org/toc/2045-2322Abstract Stem rust (caused by Puccinia graminis f. sp. tritici) is a major disease of wheat. To understand the genetic basis of stem rust resistance in Nebraska winter wheat, a set of 330 genotypes representing two nurseries (DUP2015 and TRP2015) were evaluated for resistance to a Nebraska stem rust race (QFCSC) in two replications. The TRP2015 nursery was also evaluated for its resistance to an additional 13 stem rust races. The analysis of variance revealed significant variation among genotypes in both populations for stem rust resistance. Nine stem rust genes, Sr6, Sr31, Sr1RS Amigo , Sr24, Sr36, SrTmp, Sr7b, Sr9b, and Sr38, were expected and genotyped using gene-specific markers. The results of genetic analysis confirmed the presence of seven stem rust resistance genes. One genotype (NE15680) contained target alleles for five stem rust resistance genes and had a high level of stem rust resistance against different races. Single marker analysis indicated that Sr24 and Sr38 were highly significantly associated with stem rust resistance in the DUP2015 and TRP2015 nurseries, respectively. Linkage disequilibrium analysis identified the presence of 17 SNPs in high linkage with the Sr38-specific marker. These SNPs potentially tagging the Sr38 gene could be used in marker-assisted selection after validating them in additional genetic backgrounds.Amira M. I. MouradAhmed SallamVikas BelamkarStephen WeguloGuihua BaiEzzat MahdyBahy BakheitAtif Abo El-WafaYue JinP. Stephen BaenzigerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amira M. I. Mourad Ahmed Sallam Vikas Belamkar Stephen Wegulo Guihua Bai Ezzat Mahdy Bahy Bakheit Atif Abo El-Wafa Yue Jin P. Stephen Baenziger Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm
description	Abstract Stem rust (caused by Puccinia graminis f. sp. tritici) is a major disease of wheat. To understand the genetic basis of stem rust resistance in Nebraska winter wheat, a set of 330 genotypes representing two nurseries (DUP2015 and TRP2015) were evaluated for resistance to a Nebraska stem rust race (QFCSC) in two replications. The TRP2015 nursery was also evaluated for its resistance to an additional 13 stem rust races. The analysis of variance revealed significant variation among genotypes in both populations for stem rust resistance. Nine stem rust genes, Sr6, Sr31, Sr1RS Amigo , Sr24, Sr36, SrTmp, Sr7b, Sr9b, and Sr38, were expected and genotyped using gene-specific markers. The results of genetic analysis confirmed the presence of seven stem rust resistance genes. One genotype (NE15680) contained target alleles for five stem rust resistance genes and had a high level of stem rust resistance against different races. Single marker analysis indicated that Sr24 and Sr38 were highly significantly associated with stem rust resistance in the DUP2015 and TRP2015 nurseries, respectively. Linkage disequilibrium analysis identified the presence of 17 SNPs in high linkage with the Sr38-specific marker. These SNPs potentially tagging the Sr38 gene could be used in marker-assisted selection after validating them in additional genetic backgrounds.
format	article
author	Amira M. I. Mourad Ahmed Sallam Vikas Belamkar Stephen Wegulo Guihua Bai Ezzat Mahdy Bahy Bakheit Atif Abo El-Wafa Yue Jin P. Stephen Baenziger
author_facet	Amira M. I. Mourad Ahmed Sallam Vikas Belamkar Stephen Wegulo Guihua Bai Ezzat Mahdy Bahy Bakheit Atif Abo El-Wafa Yue Jin P. Stephen Baenziger
author_sort	Amira M. I. Mourad
title	Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm
title_short	Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm
title_full	Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm
title_fullStr	Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm
title_full_unstemmed	Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm
title_sort	molecular marker dissection of stem rust resistance in nebraska bread wheat germplasm
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8699a88b10cf47c280e21c6b1b7280bb
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Molecular marker dissection of stem rust resistance in Nebraska bread wheat germplasm

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