<italic toggle="yes">Synechococcus</italic> sp. Strain PCC7002 Uses Sulfide:Quinone Oxidoreductase To Detoxify Exogenous Sulfide and To Convert Endogenous Sulfide to Cellular Sulfane Sulfur

ABSTRACT Eutrophication and deoxygenation possibly occur in coastal waters due to excessive nutrients from agricultural and aquacultural activities, leading to sulfide accumulation. Cyanobacteria, as photosynthetic prokaryotes, play significant roles in carbon fixation in the ocean. Although some cy...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daixi Liu, Jiajie Zhang, Chuanjuan Lü, Yongzhen Xia, Huaiwei Liu, Nianzhi Jiao, Luying Xun, Jihua Liu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	sulfide:quinone oxidoreductase persulfide dioxygenase cyanobacteria H2S detoxification sulfane sulfur Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/869ae316c060459589c9210abd4d72ab
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:869ae316c060459589c9210abd4d72ab
record_format	dspace
spelling	oai:doaj.org-article:869ae316c060459589c9210abd4d72ab2021-11-15T15:56:58Z<italic toggle="yes">Synechococcus</italic> sp. Strain PCC7002 Uses Sulfide:Quinone Oxidoreductase To Detoxify Exogenous Sulfide and To Convert Endogenous Sulfide to Cellular Sulfane Sulfur10.1128/mBio.03420-192150-7511https://doaj.org/article/869ae316c060459589c9210abd4d72ab2020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.03420-19https://doaj.org/toc/2150-7511ABSTRACT Eutrophication and deoxygenation possibly occur in coastal waters due to excessive nutrients from agricultural and aquacultural activities, leading to sulfide accumulation. Cyanobacteria, as photosynthetic prokaryotes, play significant roles in carbon fixation in the ocean. Although some cyanobacteria can use sulfide as the electron donor for photosynthesis under anaerobic conditions, little is known on how they interact with sulfide under aerobic conditions. In this study, we report that Synechococcus sp. strain PCC7002 (PCC7002), harboring an sqr gene encoding sulfide:quinone oxidoreductase (SQR), oxidized self-produced sulfide to S0, present as persulfide and polysulfide in the cell. The Δsqr mutant contained less cellular S0 and had increased expression of key genes involved in photosynthesis, but it was less competitive than the wild type in cocultures. Further, PCC7002 with SQR and persulfide dioxygenase (PDO) oxidized exogenous sulfide to tolerate high sulfide levels. Thus, SQR offers some benefits to cyanobacteria even under aerobic conditions, explaining the common presence of SQR in cyanobacteria. IMPORTANCE Cyanobacteria are a major force for primary production via oxygenic photosynthesis in the ocean. A marine cyanobacterium, PCC7002, is actively involved in sulfide metabolism. It uses SQR to detoxify exogenous sulfide, enabling it to survive better than its Δsqr mutant in sulfide-rich environments. PCC7002 also uses SQR to oxidize endogenously generated sulfide to S0, which is required for the proper expression of key genes involved in photosynthesis. Thus, SQR has at least two physiological functions in PCC7002. The observation provides a new perspective for the interplays of C and S cycles.Daixi LiuJiajie ZhangChuanjuan LüYongzhen XiaHuaiwei LiuNianzhi JiaoLuying XunJihua LiuAmerican Society for Microbiologyarticlesulfide:quinone oxidoreductasepersulfide dioxygenasecyanobacteriaH2S detoxificationsulfane sulfurMicrobiologyQR1-502ENmBio, Vol 11, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	sulfide:quinone oxidoreductase persulfide dioxygenase cyanobacteria H2S detoxification sulfane sulfur Microbiology QR1-502
spellingShingle	sulfide:quinone oxidoreductase persulfide dioxygenase cyanobacteria H2S detoxification sulfane sulfur Microbiology QR1-502 Daixi Liu Jiajie Zhang Chuanjuan Lü Yongzhen Xia Huaiwei Liu Nianzhi Jiao Luying Xun Jihua Liu <italic toggle="yes">Synechococcus</italic> sp. Strain PCC7002 Uses Sulfide:Quinone Oxidoreductase To Detoxify Exogenous Sulfide and To Convert Endogenous Sulfide to Cellular Sulfane Sulfur
description	ABSTRACT Eutrophication and deoxygenation possibly occur in coastal waters due to excessive nutrients from agricultural and aquacultural activities, leading to sulfide accumulation. Cyanobacteria, as photosynthetic prokaryotes, play significant roles in carbon fixation in the ocean. Although some cyanobacteria can use sulfide as the electron donor for photosynthesis under anaerobic conditions, little is known on how they interact with sulfide under aerobic conditions. In this study, we report that Synechococcus sp. strain PCC7002 (PCC7002), harboring an sqr gene encoding sulfide:quinone oxidoreductase (SQR), oxidized self-produced sulfide to S0, present as persulfide and polysulfide in the cell. The Δsqr mutant contained less cellular S0 and had increased expression of key genes involved in photosynthesis, but it was less competitive than the wild type in cocultures. Further, PCC7002 with SQR and persulfide dioxygenase (PDO) oxidized exogenous sulfide to tolerate high sulfide levels. Thus, SQR offers some benefits to cyanobacteria even under aerobic conditions, explaining the common presence of SQR in cyanobacteria. IMPORTANCE Cyanobacteria are a major force for primary production via oxygenic photosynthesis in the ocean. A marine cyanobacterium, PCC7002, is actively involved in sulfide metabolism. It uses SQR to detoxify exogenous sulfide, enabling it to survive better than its Δsqr mutant in sulfide-rich environments. PCC7002 also uses SQR to oxidize endogenously generated sulfide to S0, which is required for the proper expression of key genes involved in photosynthesis. Thus, SQR has at least two physiological functions in PCC7002. The observation provides a new perspective for the interplays of C and S cycles.
format	article
author	Daixi Liu Jiajie Zhang Chuanjuan Lü Yongzhen Xia Huaiwei Liu Nianzhi Jiao Luying Xun Jihua Liu
author_facet	Daixi Liu Jiajie Zhang Chuanjuan Lü Yongzhen Xia Huaiwei Liu Nianzhi Jiao Luying Xun Jihua Liu
author_sort	Daixi Liu
title	<italic toggle="yes">Synechococcus</italic> sp. Strain PCC7002 Uses Sulfide:Quinone Oxidoreductase To Detoxify Exogenous Sulfide and To Convert Endogenous Sulfide to Cellular Sulfane Sulfur
title_short	<italic toggle="yes">Synechococcus</italic> sp. Strain PCC7002 Uses Sulfide:Quinone Oxidoreductase To Detoxify Exogenous Sulfide and To Convert Endogenous Sulfide to Cellular Sulfane Sulfur
title_full	<italic toggle="yes">Synechococcus</italic> sp. Strain PCC7002 Uses Sulfide:Quinone Oxidoreductase To Detoxify Exogenous Sulfide and To Convert Endogenous Sulfide to Cellular Sulfane Sulfur
title_fullStr	<italic toggle="yes">Synechococcus</italic> sp. Strain PCC7002 Uses Sulfide:Quinone Oxidoreductase To Detoxify Exogenous Sulfide and To Convert Endogenous Sulfide to Cellular Sulfane Sulfur
title_full_unstemmed	<italic toggle="yes">Synechococcus</italic> sp. Strain PCC7002 Uses Sulfide:Quinone Oxidoreductase To Detoxify Exogenous Sulfide and To Convert Endogenous Sulfide to Cellular Sulfane Sulfur
title_sort	<italic toggle="yes">synechococcus</italic> sp. strain pcc7002 uses sulfide:quinone oxidoreductase to detoxify exogenous sulfide and to convert endogenous sulfide to cellular sulfane sulfur
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/869ae316c060459589c9210abd4d72ab
work_keys_str_mv	AT daixiliu italictoggleyessynechococcusitalicspstrainpcc7002usessulfidequinoneoxidoreductasetodetoxifyexogenoussulfideandtoconvertendogenoussulfidetocellularsulfanesulfur AT jiajiezhang italictoggleyessynechococcusitalicspstrainpcc7002usessulfidequinoneoxidoreductasetodetoxifyexogenoussulfideandtoconvertendogenoussulfidetocellularsulfanesulfur AT chuanjuanlu italictoggleyessynechococcusitalicspstrainpcc7002usessulfidequinoneoxidoreductasetodetoxifyexogenoussulfideandtoconvertendogenoussulfidetocellularsulfanesulfur AT yongzhenxia italictoggleyessynechococcusitalicspstrainpcc7002usessulfidequinoneoxidoreductasetodetoxifyexogenoussulfideandtoconvertendogenoussulfidetocellularsulfanesulfur AT huaiweiliu italictoggleyessynechococcusitalicspstrainpcc7002usessulfidequinoneoxidoreductasetodetoxifyexogenoussulfideandtoconvertendogenoussulfidetocellularsulfanesulfur AT nianzhijiao italictoggleyessynechococcusitalicspstrainpcc7002usessulfidequinoneoxidoreductasetodetoxifyexogenoussulfideandtoconvertendogenoussulfidetocellularsulfanesulfur AT luyingxun italictoggleyessynechococcusitalicspstrainpcc7002usessulfidequinoneoxidoreductasetodetoxifyexogenoussulfideandtoconvertendogenoussulfidetocellularsulfanesulfur AT jihualiu italictoggleyessynechococcusitalicspstrainpcc7002usessulfidequinoneoxidoreductasetodetoxifyexogenoussulfideandtoconvertendogenoussulfidetocellularsulfanesulfur
_version_	1718427101784178688

<italic toggle="yes">Synechococcus</italic> sp. Strain PCC7002 Uses Sulfide:Quinone Oxidoreductase To Detoxify Exogenous Sulfide and To Convert Endogenous Sulfide to Cellular Sulfane Sulfur

Ejemplares similares