Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock

Abstract Sepsis results from a dyshomeostatic response to infection, which may lead to hyper or hypoimmune states. Monocytes are central regulators of the inflammatory response, but our understanding of their role in the genesis and resolution of sepsis is still limited. Here, we report a comprehens...

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Autores principales:	Pedro Mendes de Azambuja Rodrigues, Richard Hemmi Valente, Giselle Villa Flor Brunoro, Helder Takashi Imoto Nakaya, Mariana Araújo-Pereira, Patricia Torres Bozza, Fernando Augusto Bozza, Monique Ramos de Oliveira Trugilho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/869df548087c49c1885abac0b2e21581
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spelling	oai:doaj.org-article:869df548087c49c1885abac0b2e215812021-12-02T16:06:41ZProteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock10.1038/s41598-021-94474-02045-2322https://doaj.org/article/869df548087c49c1885abac0b2e215812021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94474-0https://doaj.org/toc/2045-2322Abstract Sepsis results from a dyshomeostatic response to infection, which may lead to hyper or hypoimmune states. Monocytes are central regulators of the inflammatory response, but our understanding of their role in the genesis and resolution of sepsis is still limited. Here, we report a comprehensive exploration of monocyte molecular responses in a cohort of patients with septic shock via proteomic profiling. The acute stage of septic shock was associated with an impaired inflammatory phenotype, indicated by the down-regulation of MHC class II molecules and proinflammatory cytokine pathways. Simultaneously, there was an up-regulation of glycolysis enzymes and a decrease in proteins related to the citric acid cycle and oxidative phosphorylation. On the other hand, the restoration of immunocompetence was the hallmark of recovering patients, in which an upregulation of interferon signaling pathways was a notable feature. Our results provide insights into the immunopathology of sepsis and propose that, pending future studies, immunometabolism pathway components could serve as therapeutic targets in septic patients.Pedro Mendes de Azambuja RodriguesRichard Hemmi ValenteGiselle Villa Flor BrunoroHelder Takashi Imoto NakayaMariana Araújo-PereiraPatricia Torres BozzaFernando Augusto BozzaMonique Ramos de Oliveira TrugilhoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pedro Mendes de Azambuja Rodrigues Richard Hemmi Valente Giselle Villa Flor Brunoro Helder Takashi Imoto Nakaya Mariana Araújo-Pereira Patricia Torres Bozza Fernando Augusto Bozza Monique Ramos de Oliveira Trugilho Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock
description	Abstract Sepsis results from a dyshomeostatic response to infection, which may lead to hyper or hypoimmune states. Monocytes are central regulators of the inflammatory response, but our understanding of their role in the genesis and resolution of sepsis is still limited. Here, we report a comprehensive exploration of monocyte molecular responses in a cohort of patients with septic shock via proteomic profiling. The acute stage of septic shock was associated with an impaired inflammatory phenotype, indicated by the down-regulation of MHC class II molecules and proinflammatory cytokine pathways. Simultaneously, there was an up-regulation of glycolysis enzymes and a decrease in proteins related to the citric acid cycle and oxidative phosphorylation. On the other hand, the restoration of immunocompetence was the hallmark of recovering patients, in which an upregulation of interferon signaling pathways was a notable feature. Our results provide insights into the immunopathology of sepsis and propose that, pending future studies, immunometabolism pathway components could serve as therapeutic targets in septic patients.
format	article
author	Pedro Mendes de Azambuja Rodrigues Richard Hemmi Valente Giselle Villa Flor Brunoro Helder Takashi Imoto Nakaya Mariana Araújo-Pereira Patricia Torres Bozza Fernando Augusto Bozza Monique Ramos de Oliveira Trugilho
author_facet	Pedro Mendes de Azambuja Rodrigues Richard Hemmi Valente Giselle Villa Flor Brunoro Helder Takashi Imoto Nakaya Mariana Araújo-Pereira Patricia Torres Bozza Fernando Augusto Bozza Monique Ramos de Oliveira Trugilho
author_sort	Pedro Mendes de Azambuja Rodrigues
title	Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock
title_short	Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock
title_full	Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock
title_fullStr	Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock
title_full_unstemmed	Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock
title_sort	proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/869df548087c49c1885abac0b2e21581
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Proteomics reveals disturbances in the immune response and energy metabolism of monocytes from patients with septic shock

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