Assessing coral sperm motility

Abstract The declining reproductive viability of corals threatens their ability to adapt to changing ocean conditions. It is vital that we monitor this viability quantitatively and comparatively. Computer-assisted sperm analysis (CASA) systems offer in-depth analysis used regularly for domestic and...

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Autores principales:	Nikolas Zuchowicz, Jonathan Daly, Jessica Bouwmeester, Claire Lager, E. Michael Henley, C. Isabel Nuñez Lendo, Mary Hagedorn
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/86a541d1e006426d969cf8de9091eed3
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spelling	oai:doaj.org-article:86a541d1e006426d969cf8de9091eed32021-12-02T15:12:52ZAssessing coral sperm motility10.1038/s41598-020-79732-x2045-2322https://doaj.org/article/86a541d1e006426d969cf8de9091eed32021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79732-xhttps://doaj.org/toc/2045-2322Abstract The declining reproductive viability of corals threatens their ability to adapt to changing ocean conditions. It is vital that we monitor this viability quantitatively and comparatively. Computer-assisted sperm analysis (CASA) systems offer in-depth analysis used regularly for domestic and wildlife species, but not yet for coral. This study proposes quality control procedures and CASA settings that are effective for coral sperm analysis. To resolve disparities between CASA measurements and evaluations by eye, two negative effects on motility had to be resolved, slide adhesion (procedural) and sperm dilution (biological). We showed that the addition of bovine serum albumin, or caffeine, or both to fresh sperm reduced adhesion in the CASA cassettes, improved motility and motile sperm concentration (P < 0.0001), yet these additions did not affect measurements of total sperm concentration. Diluting coral sperm reduced sperm motility (P = 0.039), especially from heat-stressed corals. We found CASA concentration counts comparable to haemocytometer and flow cytometer measures (P = 0.54). We also found that motile sperm per egg is a useful predictor of fertilisation success, using cryopreserved sperm. Standard measurements of coral reproductive characteristics inform our understanding of the impacts of climate change on reef populations; this study provides a benchmark to begin this comparative work.Nikolas ZuchowiczJonathan DalyJessica BouwmeesterClaire LagerE. Michael HenleyC. Isabel Nuñez LendoMary HagedornNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nikolas Zuchowicz Jonathan Daly Jessica Bouwmeester Claire Lager E. Michael Henley C. Isabel Nuñez Lendo Mary Hagedorn Assessing coral sperm motility
description	Abstract The declining reproductive viability of corals threatens their ability to adapt to changing ocean conditions. It is vital that we monitor this viability quantitatively and comparatively. Computer-assisted sperm analysis (CASA) systems offer in-depth analysis used regularly for domestic and wildlife species, but not yet for coral. This study proposes quality control procedures and CASA settings that are effective for coral sperm analysis. To resolve disparities between CASA measurements and evaluations by eye, two negative effects on motility had to be resolved, slide adhesion (procedural) and sperm dilution (biological). We showed that the addition of bovine serum albumin, or caffeine, or both to fresh sperm reduced adhesion in the CASA cassettes, improved motility and motile sperm concentration (P < 0.0001), yet these additions did not affect measurements of total sperm concentration. Diluting coral sperm reduced sperm motility (P = 0.039), especially from heat-stressed corals. We found CASA concentration counts comparable to haemocytometer and flow cytometer measures (P = 0.54). We also found that motile sperm per egg is a useful predictor of fertilisation success, using cryopreserved sperm. Standard measurements of coral reproductive characteristics inform our understanding of the impacts of climate change on reef populations; this study provides a benchmark to begin this comparative work.
format	article
author	Nikolas Zuchowicz Jonathan Daly Jessica Bouwmeester Claire Lager E. Michael Henley C. Isabel Nuñez Lendo Mary Hagedorn
author_facet	Nikolas Zuchowicz Jonathan Daly Jessica Bouwmeester Claire Lager E. Michael Henley C. Isabel Nuñez Lendo Mary Hagedorn
author_sort	Nikolas Zuchowicz
title	Assessing coral sperm motility
title_short	Assessing coral sperm motility
title_full	Assessing coral sperm motility
title_fullStr	Assessing coral sperm motility
title_full_unstemmed	Assessing coral sperm motility
title_sort	assessing coral sperm motility
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/86a541d1e006426d969cf8de9091eed3
work_keys_str_mv	AT nikolaszuchowicz assessingcoralspermmotility AT jonathandaly assessingcoralspermmotility AT jessicabouwmeester assessingcoralspermmotility AT clairelager assessingcoralspermmotility AT emichaelhenley assessingcoralspermmotility AT cisabelnunezlendo assessingcoralspermmotility AT maryhagedorn assessingcoralspermmotility
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Assessing coral sperm motility

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