Global regulator DksA modulates virulence of Acinetobacter baumannii

DksA with (p)ppGpp regulates a wide range of gene transcriptions during the stringent response. The aim of this study was to identify a DksA ortholog in Acinetobacter baumannii and clarify the roles of DksA in bacterial physiology and virulence. The ∆dksA mutant and its complemented strains were con...

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Autores principales:	Nayeong Kim, Joo Hee Son, Kyeongmin Kim, Hyo Jeong Kim, Yoo Jeong Kim, Minsang Shin, Je Chul Lee
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	acinetobacter baumannii dksa virulence quorum sensing system biofilm formation Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/86b2fd5d484d471d96d2597df99bef41
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spelling	oai:doaj.org-article:86b2fd5d484d471d96d2597df99bef412021-11-11T14:23:42ZGlobal regulator DksA modulates virulence of Acinetobacter baumannii2150-55942150-560810.1080/21505594.2021.1995253https://doaj.org/article/86b2fd5d484d471d96d2597df99bef412021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2021.1995253https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608DksA with (p)ppGpp regulates a wide range of gene transcriptions during the stringent response. The aim of this study was to identify a DksA ortholog in Acinetobacter baumannii and clarify the roles of DksA in bacterial physiology and virulence. The ∆dksA mutant and its complemented strains were constructed using A. baumannii ATCC 17978. The AlS_0248 in A. baumannii ATCC 17978 was identified to dksA using sequence homology, protein structure prediction, and gene expression patterns under different culture conditions. The ∆dksA mutant strain showed a filamentous morphology compared with the wild-type (WT) strain. Bacterial growth was decreased in the ∆dksA mutant strain under static conditions. Surface motility was decreased in the ∆dksA mutant strain compared with the WT strain. In contrast, biofilm formation was increased and biofilm-associated genes, such as bfmR/S and csuC/D/E, were upregulated in the ∆dksA mutant strain. The ∆dksA mutant strain produced less autoinducers than the WT strain. The expression of abaI and abaR was significantly decreased in the ∆dksA mutant strain. Furthermore, the ∆dksA mutant strain showed less bacterial burden and milder histopathological changes in the lungs of mice than the WT strain. Mice survival was also significantly different between the ∆dksA mutant and WT strains. Conclusively, DksA is directly or indirectly involved in regulating a wide range of genes associated with bacterial physiology and virulence, which contributes to the pathogenesis of A. baumannii. Thus, DksA is a potential anti-virulence target for A. baumannii infection.Nayeong KimJoo Hee SonKyeongmin KimHyo Jeong KimYoo Jeong KimMinsang ShinJe Chul LeeTaylor & Francis Grouparticleacinetobacter baumanniidksavirulencequorum sensing systembiofilm formationInfectious and parasitic diseasesRC109-216ENVirulence, Vol 12, Iss 1, Pp 2750-2763 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acinetobacter baumannii dksa virulence quorum sensing system biofilm formation Infectious and parasitic diseases RC109-216
spellingShingle	acinetobacter baumannii dksa virulence quorum sensing system biofilm formation Infectious and parasitic diseases RC109-216 Nayeong Kim Joo Hee Son Kyeongmin Kim Hyo Jeong Kim Yoo Jeong Kim Minsang Shin Je Chul Lee Global regulator DksA modulates virulence of Acinetobacter baumannii
description	DksA with (p)ppGpp regulates a wide range of gene transcriptions during the stringent response. The aim of this study was to identify a DksA ortholog in Acinetobacter baumannii and clarify the roles of DksA in bacterial physiology and virulence. The ∆dksA mutant and its complemented strains were constructed using A. baumannii ATCC 17978. The AlS_0248 in A. baumannii ATCC 17978 was identified to dksA using sequence homology, protein structure prediction, and gene expression patterns under different culture conditions. The ∆dksA mutant strain showed a filamentous morphology compared with the wild-type (WT) strain. Bacterial growth was decreased in the ∆dksA mutant strain under static conditions. Surface motility was decreased in the ∆dksA mutant strain compared with the WT strain. In contrast, biofilm formation was increased and biofilm-associated genes, such as bfmR/S and csuC/D/E, were upregulated in the ∆dksA mutant strain. The ∆dksA mutant strain produced less autoinducers than the WT strain. The expression of abaI and abaR was significantly decreased in the ∆dksA mutant strain. Furthermore, the ∆dksA mutant strain showed less bacterial burden and milder histopathological changes in the lungs of mice than the WT strain. Mice survival was also significantly different between the ∆dksA mutant and WT strains. Conclusively, DksA is directly or indirectly involved in regulating a wide range of genes associated with bacterial physiology and virulence, which contributes to the pathogenesis of A. baumannii. Thus, DksA is a potential anti-virulence target for A. baumannii infection.
format	article
author	Nayeong Kim Joo Hee Son Kyeongmin Kim Hyo Jeong Kim Yoo Jeong Kim Minsang Shin Je Chul Lee
author_facet	Nayeong Kim Joo Hee Son Kyeongmin Kim Hyo Jeong Kim Yoo Jeong Kim Minsang Shin Je Chul Lee
author_sort	Nayeong Kim
title	Global regulator DksA modulates virulence of Acinetobacter baumannii
title_short	Global regulator DksA modulates virulence of Acinetobacter baumannii
title_full	Global regulator DksA modulates virulence of Acinetobacter baumannii
title_fullStr	Global regulator DksA modulates virulence of Acinetobacter baumannii
title_full_unstemmed	Global regulator DksA modulates virulence of Acinetobacter baumannii
title_sort	global regulator dksa modulates virulence of acinetobacter baumannii
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/86b2fd5d484d471d96d2597df99bef41
work_keys_str_mv	AT nayeongkim globalregulatordksamodulatesvirulenceofacinetobacterbaumannii AT jooheeson globalregulatordksamodulatesvirulenceofacinetobacterbaumannii AT kyeongminkim globalregulatordksamodulatesvirulenceofacinetobacterbaumannii AT hyojeongkim globalregulatordksamodulatesvirulenceofacinetobacterbaumannii AT yoojeongkim globalregulatordksamodulatesvirulenceofacinetobacterbaumannii AT minsangshin globalregulatordksamodulatesvirulenceofacinetobacterbaumannii AT jechullee globalregulatordksamodulatesvirulenceofacinetobacterbaumannii
_version_	1718438954016964608

Global regulator DksA modulates virulence of Acinetobacter baumannii

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