Co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells

Macrophages can be polarized by in vitro culture stimuli into M1 or M2 cells, but microenvironments in vivo are more complex. Here the authors analyze cultured macrophages stimulated with a combination of M1 and M2 stimuli by single-cell RNA sequencing, machine learning, and single-cell secretion pr...

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Autores principales:	Andrés R. Muñoz-Rojas, Ilana Kelsey, Jenna L. Pappalardo, Meibin Chen, Kathryn Miller-Jensen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/86bb0282c387410a83e2eea9693c147b
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spelling	oai:doaj.org-article:86bb0282c387410a83e2eea9693c147b2021-12-02T14:11:51ZCo-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells10.1038/s41467-020-20540-22041-1723https://doaj.org/article/86bb0282c387410a83e2eea9693c147b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20540-2https://doaj.org/toc/2041-1723Macrophages can be polarized by in vitro culture stimuli into M1 or M2 cells, but microenvironments in vivo are more complex. Here the authors analyze cultured macrophages stimulated with a combination of M1 and M2 stimuli by single-cell RNA sequencing, machine learning, and single-cell secretion profiling to show a surprising level of heterogeneity of response.Andrés R. Muñoz-RojasIlana KelseyJenna L. PappalardoMeibin ChenKathryn Miller-JensenNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Andrés R. Muñoz-Rojas Ilana Kelsey Jenna L. Pappalardo Meibin Chen Kathryn Miller-Jensen Co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells
description	Macrophages can be polarized by in vitro culture stimuli into M1 or M2 cells, but microenvironments in vivo are more complex. Here the authors analyze cultured macrophages stimulated with a combination of M1 and M2 stimuli by single-cell RNA sequencing, machine learning, and single-cell secretion profiling to show a surprising level of heterogeneity of response.
format	article
author	Andrés R. Muñoz-Rojas Ilana Kelsey Jenna L. Pappalardo Meibin Chen Kathryn Miller-Jensen
author_facet	Andrés R. Muñoz-Rojas Ilana Kelsey Jenna L. Pappalardo Meibin Chen Kathryn Miller-Jensen
author_sort	Andrés R. Muñoz-Rojas
title	Co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells
title_short	Co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells
title_full	Co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells
title_fullStr	Co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells
title_full_unstemmed	Co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells
title_sort	co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/86bb0282c387410a83e2eea9693c147b
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Co-stimulation with opposing macrophage polarization cues leads to orthogonal secretion programs in individual cells

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