PROMOTER REGION rs1800629 TNFα POLYMORPHISM AND ITS INFLUENCE ON TUMOR NECROSIS FACTOR ALPHA CONCENTRATION IN BLOOD OF HEALTHY INDIVIDUALS AND PATIENTS WITH ERYSIPELAS

Erysipelas is an actual problem of health care. It is characterized by consistently high morbidity, excepted tendency to recur, reduced the life quality and took important place in the structure of temporary disability. The changes of immunological reactivity, depended on genetic characteristics of...

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Autores principales:	A. S. Emelyanov, A. N. Emelyanova, B. S. Pushkarev, Yu. A. Vitkovsky
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	erysipelas genetic polymorphism tnfα cytokines inflammation rs1800629 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/86c4276d04404e02b64a8abbc69bf242
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spelling	oai:doaj.org-article:86c4276d04404e02b64a8abbc69bf2422021-11-18T08:03:47ZPROMOTER REGION rs1800629 TNFα POLYMORPHISM AND ITS INFLUENCE ON TUMOR NECROSIS FACTOR ALPHA CONCENTRATION IN BLOOD OF HEALTHY INDIVIDUALS AND PATIENTS WITH ERYSIPELAS1563-06252313-741X10.15789/1563-0625-2018-3-411-416https://doaj.org/article/86c4276d04404e02b64a8abbc69bf2422018-06-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1531https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XErysipelas is an actual problem of health care. It is characterized by consistently high morbidity, excepted tendency to recur, reduced the life quality and took important place in the structure of temporary disability. The changes of immunological reactivity, depended on genetic characteristics of the individual were established to play important role in the pathogenesis of erysipelas. The prediction of the course of erysipelas is unresolved problem yet. The genetic features of the organism, such as the genetic polymorphisms of some cytokines, involved on the prediction of the course of erysipelas. Investigations of TNFα genes demonstrated that their transcriptions increased and productions enhanced. Aim was to study the influence of polymorphism of TNFα gene promoter rs1800629 on the concentration of TNFα in the blood of healthy individuals and patients with erysipelas in primary and recurrent course. The study was performed at 104 patients with erysipelas (54 patients with primary erysipelas and 50 patients with recurrent course) and 94 healthy residents. Gene polymorphism of TNFα rs1800629 was detected by PCR method. Measurement of the concentration of TNFα was performed by immunoassay analysis. Homo- and heterozygous SNP of the TNFα rs1800629 gene were conformed to Hardy–Weinberg equilibrium (p > 0.05). Minor allele A was found to be registered in erysipelas patient less frequently by 2.9 times than in healthy individuals. The patients who carried homozygous G/G were observed in 88.7% of cases. At that heterozygous G/A were registered in any case. In other case, among the patients, no cases of the carriage of the mutant genotypes A/A were detected. Thus, allele G and genotypes G/G of promoter gene of TNFα rs1800629 predisposed to erysipelas. Heterozygous G/A of promoter gene of TNFα rs1800629 increased the risk of erysipelas. G-allele carrier led to decrease of TNFα concentration homozygous G/G patients.A. S. EmelyanovA. N. EmelyanovaB. S. PushkarevYu. A. VitkovskySPb RAACIarticleerysipelasgenetic polymorphismtnfαcytokinesinflammationrs1800629Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 3, Pp 411-416 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	erysipelas genetic polymorphism tnfα cytokines inflammation rs1800629 Immunologic diseases. Allergy RC581-607
spellingShingle	erysipelas genetic polymorphism tnfα cytokines inflammation rs1800629 Immunologic diseases. Allergy RC581-607 A. S. Emelyanov A. N. Emelyanova B. S. Pushkarev Yu. A. Vitkovsky PROMOTER REGION rs1800629 TNFα POLYMORPHISM AND ITS INFLUENCE ON TUMOR NECROSIS FACTOR ALPHA CONCENTRATION IN BLOOD OF HEALTHY INDIVIDUALS AND PATIENTS WITH ERYSIPELAS
description	Erysipelas is an actual problem of health care. It is characterized by consistently high morbidity, excepted tendency to recur, reduced the life quality and took important place in the structure of temporary disability. The changes of immunological reactivity, depended on genetic characteristics of the individual were established to play important role in the pathogenesis of erysipelas. The prediction of the course of erysipelas is unresolved problem yet. The genetic features of the organism, such as the genetic polymorphisms of some cytokines, involved on the prediction of the course of erysipelas. Investigations of TNFα genes demonstrated that their transcriptions increased and productions enhanced. Aim was to study the influence of polymorphism of TNFα gene promoter rs1800629 on the concentration of TNFα in the blood of healthy individuals and patients with erysipelas in primary and recurrent course. The study was performed at 104 patients with erysipelas (54 patients with primary erysipelas and 50 patients with recurrent course) and 94 healthy residents. Gene polymorphism of TNFα rs1800629 was detected by PCR method. Measurement of the concentration of TNFα was performed by immunoassay analysis. Homo- and heterozygous SNP of the TNFα rs1800629 gene were conformed to Hardy–Weinberg equilibrium (p > 0.05). Minor allele A was found to be registered in erysipelas patient less frequently by 2.9 times than in healthy individuals. The patients who carried homozygous G/G were observed in 88.7% of cases. At that heterozygous G/A were registered in any case. In other case, among the patients, no cases of the carriage of the mutant genotypes A/A were detected. Thus, allele G and genotypes G/G of promoter gene of TNFα rs1800629 predisposed to erysipelas. Heterozygous G/A of promoter gene of TNFα rs1800629 increased the risk of erysipelas. G-allele carrier led to decrease of TNFα concentration homozygous G/G patients.
format	article
author	A. S. Emelyanov A. N. Emelyanova B. S. Pushkarev Yu. A. Vitkovsky
author_facet	A. S. Emelyanov A. N. Emelyanova B. S. Pushkarev Yu. A. Vitkovsky
author_sort	A. S. Emelyanov
title	PROMOTER REGION rs1800629 TNFα POLYMORPHISM AND ITS INFLUENCE ON TUMOR NECROSIS FACTOR ALPHA CONCENTRATION IN BLOOD OF HEALTHY INDIVIDUALS AND PATIENTS WITH ERYSIPELAS
title_short	PROMOTER REGION rs1800629 TNFα POLYMORPHISM AND ITS INFLUENCE ON TUMOR NECROSIS FACTOR ALPHA CONCENTRATION IN BLOOD OF HEALTHY INDIVIDUALS AND PATIENTS WITH ERYSIPELAS
title_full	PROMOTER REGION rs1800629 TNFα POLYMORPHISM AND ITS INFLUENCE ON TUMOR NECROSIS FACTOR ALPHA CONCENTRATION IN BLOOD OF HEALTHY INDIVIDUALS AND PATIENTS WITH ERYSIPELAS
title_fullStr	PROMOTER REGION rs1800629 TNFα POLYMORPHISM AND ITS INFLUENCE ON TUMOR NECROSIS FACTOR ALPHA CONCENTRATION IN BLOOD OF HEALTHY INDIVIDUALS AND PATIENTS WITH ERYSIPELAS
title_full_unstemmed	PROMOTER REGION rs1800629 TNFα POLYMORPHISM AND ITS INFLUENCE ON TUMOR NECROSIS FACTOR ALPHA CONCENTRATION IN BLOOD OF HEALTHY INDIVIDUALS AND PATIENTS WITH ERYSIPELAS
title_sort	promoter region rs1800629 tnfα polymorphism and its influence on tumor necrosis factor alpha concentration in blood of healthy individuals and patients with erysipelas
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/86c4276d04404e02b64a8abbc69bf242
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PROMOTER REGION rs1800629 TNFα POLYMORPHISM AND ITS INFLUENCE ON TUMOR NECROSIS FACTOR ALPHA CONCENTRATION IN BLOOD OF HEALTHY INDIVIDUALS AND PATIENTS WITH ERYSIPELAS

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