Callus induction and regeneration in sugarcane under drought stress

Tissue culture methods are useful in assessing the tolerance of various stresses due to the ease of controlling stress under in vitro conditions. This study aimed to investigate the response of sugarcane genotyps to drought stress using calli as a model system. For inducing sugarcane callus, the med...

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Autores principales:	Nader R. Abdelsalam, Wafaa E. Grad, Nabawya S.A. Ghura, Ahmed E. Khalid, Rehab Y. Ghareeb, El-Sayed M. Desoky, Mostafa M. Rady, Hatim M. Al-Yasi, Esmat F. Ali
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Water scarcity Saccharum officinarum Tissue culture Development Mass propagation Regeneration Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/86c9a479ad27434aa2d961f93eff3613
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spelling	oai:doaj.org-article:86c9a479ad27434aa2d961f93eff36132021-11-20T04:57:08ZCallus induction and regeneration in sugarcane under drought stress1319-562X10.1016/j.sjbs.2021.08.047https://doaj.org/article/86c9a479ad27434aa2d961f93eff36132021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21007361https://doaj.org/toc/1319-562XTissue culture methods are useful in assessing the tolerance of various stresses due to the ease of controlling stress under in vitro conditions. This study aimed to investigate the response of sugarcane genotyps to drought stress using calli as a model system. For inducing sugarcane callus, the medium of Murashige and Skoog (MS) was used with different mannitol concentrations (100, 200, and 300 mM) to measure their effects on callus frequency, the day of callus initiation, embryogenic potential, relative growth rate (RGR), water and proline contents, K+ and Na+ contents, as well as the formation of shoot and roots for three sugarcane genotypes (e.g., GT 54-9, G 84-47, and pH 8013). The RAPD-PCR analysis was carried out using five oligonucleotide primers to identify the genetic variation among sugarcane genotypes. The results indicated that the degree of callus proliferation varied from 70 − 86%. The highest value of callus proliferation, PGR, shoot formation was recorded for the genotype GT 54-9 compared to the other two genotypes (G 84-47 and pH 8013). Calli treated with 100 mM mannitol showed the highest RGR, proline and waer contents for the genotype GT 54-9, while, those treated with 300 mM recorded the lowest values of these parameters for the genotype pH 8013. The genotype G 84-47 collected highest Na+ content, while the genotype pH 8013 collected highest K+ content. The results of this study recommend preference for GT 54-9 genotype, which is considered the most promising genotype, showing more tolerance to drought stress based on all studied traits.Nader R. AbdelsalamWafaa E. GradNabawya S.A. GhuraAhmed E. KhalidRehab Y. GhareebEl-Sayed M. DesokyMostafa M. RadyHatim M. Al-YasiEsmat F. AliElsevierarticleWater scarcitySaccharum officinarumTissue cultureDevelopmentMass propagationRegenerationBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7432-7442 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Water scarcity Saccharum officinarum Tissue culture Development Mass propagation Regeneration Biology (General) QH301-705.5
spellingShingle	Water scarcity Saccharum officinarum Tissue culture Development Mass propagation Regeneration Biology (General) QH301-705.5 Nader R. Abdelsalam Wafaa E. Grad Nabawya S.A. Ghura Ahmed E. Khalid Rehab Y. Ghareeb El-Sayed M. Desoky Mostafa M. Rady Hatim M. Al-Yasi Esmat F. Ali Callus induction and regeneration in sugarcane under drought stress
description	Tissue culture methods are useful in assessing the tolerance of various stresses due to the ease of controlling stress under in vitro conditions. This study aimed to investigate the response of sugarcane genotyps to drought stress using calli as a model system. For inducing sugarcane callus, the medium of Murashige and Skoog (MS) was used with different mannitol concentrations (100, 200, and 300 mM) to measure their effects on callus frequency, the day of callus initiation, embryogenic potential, relative growth rate (RGR), water and proline contents, K+ and Na+ contents, as well as the formation of shoot and roots for three sugarcane genotypes (e.g., GT 54-9, G 84-47, and pH 8013). The RAPD-PCR analysis was carried out using five oligonucleotide primers to identify the genetic variation among sugarcane genotypes. The results indicated that the degree of callus proliferation varied from 70 − 86%. The highest value of callus proliferation, PGR, shoot formation was recorded for the genotype GT 54-9 compared to the other two genotypes (G 84-47 and pH 8013). Calli treated with 100 mM mannitol showed the highest RGR, proline and waer contents for the genotype GT 54-9, while, those treated with 300 mM recorded the lowest values of these parameters for the genotype pH 8013. The genotype G 84-47 collected highest Na+ content, while the genotype pH 8013 collected highest K+ content. The results of this study recommend preference for GT 54-9 genotype, which is considered the most promising genotype, showing more tolerance to drought stress based on all studied traits.
format	article
author	Nader R. Abdelsalam Wafaa E. Grad Nabawya S.A. Ghura Ahmed E. Khalid Rehab Y. Ghareeb El-Sayed M. Desoky Mostafa M. Rady Hatim M. Al-Yasi Esmat F. Ali
author_facet	Nader R. Abdelsalam Wafaa E. Grad Nabawya S.A. Ghura Ahmed E. Khalid Rehab Y. Ghareeb El-Sayed M. Desoky Mostafa M. Rady Hatim M. Al-Yasi Esmat F. Ali
author_sort	Nader R. Abdelsalam
title	Callus induction and regeneration in sugarcane under drought stress
title_short	Callus induction and regeneration in sugarcane under drought stress
title_full	Callus induction and regeneration in sugarcane under drought stress
title_fullStr	Callus induction and regeneration in sugarcane under drought stress
title_full_unstemmed	Callus induction and regeneration in sugarcane under drought stress
title_sort	callus induction and regeneration in sugarcane under drought stress
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/86c9a479ad27434aa2d961f93eff3613
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Callus induction and regeneration in sugarcane under drought stress

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