Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode

Abstract Inactivation technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is certainly a critical measure to mitigate the spread of coronavirus disease 2019 (COVID-19). A deep ultraviolet light-emitting diode (DUV-LED) would be a promising candidate to inactivate SARS-CoV-2,...

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Autores principales:	Takeo Minamikawa, Takaaki Koma, Akihiro Suzuki, Takahiko Mizuno, Kentaro Nagamatsu, Hideki Arimochi, Koichiro Tsuchiya, Kaoru Matsuoka, Takeshi Yasui, Koji Yasutomo, Masako Nomaguchi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/86cd4db202ac40e981c77988641100fd
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spelling	oai:doaj.org-article:86cd4db202ac40e981c77988641100fd2021-12-02T13:35:05ZQuantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode10.1038/s41598-021-84592-02045-2322https://doaj.org/article/86cd4db202ac40e981c77988641100fd2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84592-0https://doaj.org/toc/2045-2322Abstract Inactivation technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is certainly a critical measure to mitigate the spread of coronavirus disease 2019 (COVID-19). A deep ultraviolet light-emitting diode (DUV-LED) would be a promising candidate to inactivate SARS-CoV-2, based on the well-known antiviral effects of DUV on microorganisms and viruses. However, due to variations in the inactivation effects across different viruses, quantitative evaluations of the inactivation profile of SARS-CoV-2 by DUV-LED irradiation need to be performed. In the present study, we quantify the irradiation dose of DUV-LED necessary to inactivate SARS-CoV-2. For this purpose, we determined the culture media suitable for the irradiation of SARS-CoV-2 and optimized the irradiation apparatus using commercially available DUV-LEDs that operate at a center wavelength of 265, 280, or 300 nm. Under these conditions, we successfully analyzed the relationship between SARS-CoV-2 infectivity and the irradiation dose of the DUV-LEDs at each wavelength without irrelevant biological effects. In conclusion, total doses of 1.8 mJ/cm2 for 265 nm, 3.0 mJ/cm2 for 280 nm, and 23 mJ/cm2 for 300 nm are required to inactivate 99.9% of SARS-CoV-2. Our results provide quantitative antiviral effects of DUV irradiation on SARS-CoV-2, serving as basic knowledge of inactivation technologies against SARS-CoV-2.Takeo MinamikawaTakaaki KomaAkihiro SuzukiTakahiko MizunoKentaro NagamatsuHideki ArimochiKoichiro TsuchiyaKaoru MatsuokaTakeshi YasuiKoji YasutomoMasako NomaguchiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takeo Minamikawa Takaaki Koma Akihiro Suzuki Takahiko Mizuno Kentaro Nagamatsu Hideki Arimochi Koichiro Tsuchiya Kaoru Matsuoka Takeshi Yasui Koji Yasutomo Masako Nomaguchi Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
description	Abstract Inactivation technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is certainly a critical measure to mitigate the spread of coronavirus disease 2019 (COVID-19). A deep ultraviolet light-emitting diode (DUV-LED) would be a promising candidate to inactivate SARS-CoV-2, based on the well-known antiviral effects of DUV on microorganisms and viruses. However, due to variations in the inactivation effects across different viruses, quantitative evaluations of the inactivation profile of SARS-CoV-2 by DUV-LED irradiation need to be performed. In the present study, we quantify the irradiation dose of DUV-LED necessary to inactivate SARS-CoV-2. For this purpose, we determined the culture media suitable for the irradiation of SARS-CoV-2 and optimized the irradiation apparatus using commercially available DUV-LEDs that operate at a center wavelength of 265, 280, or 300 nm. Under these conditions, we successfully analyzed the relationship between SARS-CoV-2 infectivity and the irradiation dose of the DUV-LEDs at each wavelength without irrelevant biological effects. In conclusion, total doses of 1.8 mJ/cm2 for 265 nm, 3.0 mJ/cm2 for 280 nm, and 23 mJ/cm2 for 300 nm are required to inactivate 99.9% of SARS-CoV-2. Our results provide quantitative antiviral effects of DUV irradiation on SARS-CoV-2, serving as basic knowledge of inactivation technologies against SARS-CoV-2.
format	article
author	Takeo Minamikawa Takaaki Koma Akihiro Suzuki Takahiko Mizuno Kentaro Nagamatsu Hideki Arimochi Koichiro Tsuchiya Kaoru Matsuoka Takeshi Yasui Koji Yasutomo Masako Nomaguchi
author_facet	Takeo Minamikawa Takaaki Koma Akihiro Suzuki Takahiko Mizuno Kentaro Nagamatsu Hideki Arimochi Koichiro Tsuchiya Kaoru Matsuoka Takeshi Yasui Koji Yasutomo Masako Nomaguchi
author_sort	Takeo Minamikawa
title	Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_short	Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_full	Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_fullStr	Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_full_unstemmed	Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode
title_sort	quantitative evaluation of sars-cov-2 inactivation using a deep ultraviolet light-emitting diode
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/86cd4db202ac40e981c77988641100fd
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Quantitative evaluation of SARS-CoV-2 inactivation using a deep ultraviolet light-emitting diode

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