Detoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases

This present study aims to evaluate the peroxidase activity in soybean extract which was obtained through the seeds of the soybean. This enzymatic extract (Soybean Peroxidase-SBP) was then compared with commercially available pure enzyme i.e., horseradish peroxidase (HRP). The effect of the enzyme c...

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Autores principales:	Shweta Sharma, Nithya Rani R, Vadiraj K.T., Ravi M.B., Chandan Shivamallu, Raghu Ram Achar
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Soybean peroxidase (SBP) Decolorization Sunset yellow Coomassie brilliant blue R-250 (CBB) Horseradish peroxidase (HRP) Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/86d144cf6df54595b26057b97dd6d13e
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spelling	oai:doaj.org-article:86d144cf6df54595b26057b97dd6d13e2021-11-18T04:51:34ZDetoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases2666-086510.1016/j.crgsc.2021.100215https://doaj.org/article/86d144cf6df54595b26057b97dd6d13e2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666086521001624https://doaj.org/toc/2666-0865This present study aims to evaluate the peroxidase activity in soybean extract which was obtained through the seeds of the soybean. This enzymatic extract (Soybean Peroxidase-SBP) was then compared with commercially available pure enzyme i.e., horseradish peroxidase (HRP). The effect of the enzyme concentration, substrate concentration, pH temperature were studied to interpret the factors affectingthe rate of the reaction. Therefore, after the studies were carried out, it was detected that the increase in the concentration of the substrate as well as enzyme resulted in the gradual increase of the activity. The rate of the reaction thus became constant when all the enzymes got saturated after a certain range. The optimum pH and temperature of 7 and 40 °C was maintained for the decolorization of the synthetic dyes viz., Sunset yellow and Coomassie Brilliant blue dye R-250. The removal of Sunset yellow dye and CBB was around 45 % and 11 %, respectively and results were compared with the degradation with HRP in similar conditions. The effective degradation of Sunset yellow over CBB may be because of the difference in the molecular weight. Furthermore, optimization of reaction mixture and reaction conditions is in progress. The results obtained in this work suggest that peroxidases can be used in a supplementary step to the conventional processes for specific dye removal.Shweta SharmaNithya Rani RVadiraj K.T.Ravi M.B.Chandan ShivamalluRaghu Ram AcharElsevierarticleSoybean peroxidase (SBP)DecolorizationSunset yellowCoomassie brilliant blue R-250 (CBB)Horseradish peroxidase (HRP)ChemistryQD1-999ENCurrent Research in Green and Sustainable Chemistry, Vol 4, Iss , Pp 100215- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Soybean peroxidase (SBP) Decolorization Sunset yellow Coomassie brilliant blue R-250 (CBB) Horseradish peroxidase (HRP) Chemistry QD1-999
spellingShingle	Soybean peroxidase (SBP) Decolorization Sunset yellow Coomassie brilliant blue R-250 (CBB) Horseradish peroxidase (HRP) Chemistry QD1-999 Shweta Sharma Nithya Rani R Vadiraj K.T. Ravi M.B. Chandan Shivamallu Raghu Ram Achar Detoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases
description	This present study aims to evaluate the peroxidase activity in soybean extract which was obtained through the seeds of the soybean. This enzymatic extract (Soybean Peroxidase-SBP) was then compared with commercially available pure enzyme i.e., horseradish peroxidase (HRP). The effect of the enzyme concentration, substrate concentration, pH temperature were studied to interpret the factors affectingthe rate of the reaction. Therefore, after the studies were carried out, it was detected that the increase in the concentration of the substrate as well as enzyme resulted in the gradual increase of the activity. The rate of the reaction thus became constant when all the enzymes got saturated after a certain range. The optimum pH and temperature of 7 and 40 °C was maintained for the decolorization of the synthetic dyes viz., Sunset yellow and Coomassie Brilliant blue dye R-250. The removal of Sunset yellow dye and CBB was around 45 % and 11 %, respectively and results were compared with the degradation with HRP in similar conditions. The effective degradation of Sunset yellow over CBB may be because of the difference in the molecular weight. Furthermore, optimization of reaction mixture and reaction conditions is in progress. The results obtained in this work suggest that peroxidases can be used in a supplementary step to the conventional processes for specific dye removal.
format	article
author	Shweta Sharma Nithya Rani R Vadiraj K.T. Ravi M.B. Chandan Shivamallu Raghu Ram Achar
author_facet	Shweta Sharma Nithya Rani R Vadiraj K.T. Ravi M.B. Chandan Shivamallu Raghu Ram Achar
author_sort	Shweta Sharma
title	Detoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases
title_short	Detoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases
title_full	Detoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases
title_fullStr	Detoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases
title_full_unstemmed	Detoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases
title_sort	detoxification of sunset yellow and brilliant blue dyes using soybean peroxidases
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/86d144cf6df54595b26057b97dd6d13e
work_keys_str_mv	AT shwetasharma detoxificationofsunsetyellowandbrilliantbluedyesusingsoybeanperoxidases AT nithyaranir detoxificationofsunsetyellowandbrilliantbluedyesusingsoybeanperoxidases AT vadirajkt detoxificationofsunsetyellowandbrilliantbluedyesusingsoybeanperoxidases AT ravimb detoxificationofsunsetyellowandbrilliantbluedyesusingsoybeanperoxidases AT chandanshivamallu detoxificationofsunsetyellowandbrilliantbluedyesusingsoybeanperoxidases AT raghuramachar detoxificationofsunsetyellowandbrilliantbluedyesusingsoybeanperoxidases
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Detoxification of Sunset yellow and Brilliant blue dyes using Soybean peroxidases

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