Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice

Abstract Irreversible electroporation (IRE) is a non-thermal tissue ablative technology that has emerging applications in surgical oncology and regenerative surgery. To advance its therapeutic usefulness, it is important to understand the mechanisms through which IRE induces cell death and the role...

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Autores principales:	Maya Lopez-Ichikawa, Ngan K. Vu, Amar Nijagal, Boris Rubinsky, Tammy T. Chang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/86dc7c5d9332411f95eb75efd22e41ed
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spelling	oai:doaj.org-article:86dc7c5d9332411f95eb75efd22e41ed2021-12-02T17:55:13ZNeutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice10.1038/s41598-021-94016-82045-2322https://doaj.org/article/86dc7c5d9332411f95eb75efd22e41ed2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94016-8https://doaj.org/toc/2045-2322Abstract Irreversible electroporation (IRE) is a non-thermal tissue ablative technology that has emerging applications in surgical oncology and regenerative surgery. To advance its therapeutic usefulness, it is important to understand the mechanisms through which IRE induces cell death and the role of the innate immune system in mediating subsequent regenerative repair. Through intravital imaging of the liver in mice, we show that IRE produces distinctive tissue injury features, including delayed yet robust recruitment of neutrophils, consistent with programmed necrosis. IRE treatment converts the monocyte/macrophage balance from pro-inflammatory to pro-reparative populations, and depletion of neutrophils inhibits this conversion. Reduced generation of pro-reparative Ly6CloF4/80hi macrophages correlates with lower numbers of SOX9+ hepatic progenitor cells in areas of macrophage clusters within the IRE injury zone. Our findings suggest that neutrophils play an important role in promoting the development of pro-reparative Ly6Clo monocytes/macrophages at the site of IRE injury, thus establishing conditions of regenerative repair.Maya Lopez-IchikawaNgan K. VuAmar NijagalBoris RubinskyTammy T. ChangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maya Lopez-Ichikawa Ngan K. Vu Amar Nijagal Boris Rubinsky Tammy T. Chang Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice
description	Abstract Irreversible electroporation (IRE) is a non-thermal tissue ablative technology that has emerging applications in surgical oncology and regenerative surgery. To advance its therapeutic usefulness, it is important to understand the mechanisms through which IRE induces cell death and the role of the innate immune system in mediating subsequent regenerative repair. Through intravital imaging of the liver in mice, we show that IRE produces distinctive tissue injury features, including delayed yet robust recruitment of neutrophils, consistent with programmed necrosis. IRE treatment converts the monocyte/macrophage balance from pro-inflammatory to pro-reparative populations, and depletion of neutrophils inhibits this conversion. Reduced generation of pro-reparative Ly6CloF4/80hi macrophages correlates with lower numbers of SOX9+ hepatic progenitor cells in areas of macrophage clusters within the IRE injury zone. Our findings suggest that neutrophils play an important role in promoting the development of pro-reparative Ly6Clo monocytes/macrophages at the site of IRE injury, thus establishing conditions of regenerative repair.
format	article
author	Maya Lopez-Ichikawa Ngan K. Vu Amar Nijagal Boris Rubinsky Tammy T. Chang
author_facet	Maya Lopez-Ichikawa Ngan K. Vu Amar Nijagal Boris Rubinsky Tammy T. Chang
author_sort	Maya Lopez-Ichikawa
title	Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice
title_short	Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice
title_full	Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice
title_fullStr	Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice
title_full_unstemmed	Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice
title_sort	neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/86dc7c5d9332411f95eb75efd22e41ed
work_keys_str_mv	AT mayalopezichikawa neutrophilsareimportantforthedevelopmentofproreparativemacrophagesafterirreversibleelectroporationoftheliverinmice AT ngankvu neutrophilsareimportantforthedevelopmentofproreparativemacrophagesafterirreversibleelectroporationoftheliverinmice AT amarnijagal neutrophilsareimportantforthedevelopmentofproreparativemacrophagesafterirreversibleelectroporationoftheliverinmice AT borisrubinsky neutrophilsareimportantforthedevelopmentofproreparativemacrophagesafterirreversibleelectroporationoftheliverinmice AT tammytchang neutrophilsareimportantforthedevelopmentofproreparativemacrophagesafterirreversibleelectroporationoftheliverinmice
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Neutrophils are important for the development of pro-reparative macrophages after irreversible electroporation of the liver in mice

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