Nontypeable Haemophilus influenzae genetic islands associated with chronic pulmonary infection.

<h4>Background</h4>Haemophilus influenzae (Hi) colonizes the human respiratory tract and is an important pathogen associated with chronic obstructive pulmonary disease (COPD). Bacterial factors that interact with the human host may be important in the pathogenesis of COPD. These factors,...

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Autores principales:	Lixin Zhang, Jingping Xie, Mayuri Patel, Arsala Bakhtyar, Garth D Ehrlich, Azad Ahmed, Josh Earl, Carl F Marrs, Daniel Clemans, Timothy F Murphy, Janet R Gilsdorf
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/86e36140375842389a45b4a3ba0d2ad5
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spelling	oai:doaj.org-article:86e36140375842389a45b4a3ba0d2ad52021-11-18T07:06:19ZNontypeable Haemophilus influenzae genetic islands associated with chronic pulmonary infection.1932-620310.1371/journal.pone.0044730https://doaj.org/article/86e36140375842389a45b4a3ba0d2ad52012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22970300/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Haemophilus influenzae (Hi) colonizes the human respiratory tract and is an important pathogen associated with chronic obstructive pulmonary disease (COPD). Bacterial factors that interact with the human host may be important in the pathogenesis of COPD. These factors, however, have not been well defined. The overall goal of this study was to identify bacterial genetic elements with increased prevalence among H. influenzae strains isolated from patients with COPD compared to those isolated from the pharynges of healthy individuals.<h4>Methodology/principal findings</h4>Four nontypeable H. influenzae (NTHi) strains, two isolated from the airways of patients with COPD and two from a healthy individual, were subjected to whole genome sequencing using 454 FLX Titanium technology. COPD strain-specific genetic islands greater than 500 bp in size were identified by in silico subtraction. Open reading frames residing within these islands include known Hi virulence genes such as lic2b, hgbA, iga, hmw1 and hmw2, as well as genes encoding urease and other enzymes involving metabolic pathways. The distributions of seven selected genetic islands were assessed among a panel of 421 NTHi strains of both disease and commensal origins using a Library-on-a-Slide high throughput dot blot DNA hybridization procedure. Four of the seven islands screened, containing genes that encode a methyltransferase, a dehydrogenase, a urease synthesis enzyme, and a set of unknown short ORFs, respectively, were more prevalent in COPD strains than in colonizing strains with prevalence ratios ranging from 1.21 to 2.85 (p ≤ 0.0002). Surprisingly, none of these sequences show increased prevalence among NTHi isolated from the airways of patients with cystic fibrosis.<h4>Conclusions/significance</h4>Our data suggest that specific bacterial genes, many involved in metabolic functions, are associated with the ability of NTHi strains to survive in the lower airways of patients with COPD.Lixin ZhangJingping XieMayuri PatelArsala BakhtyarGarth D EhrlichAzad AhmedJosh EarlCarl F MarrsDaniel ClemansTimothy F MurphyJanet R GilsdorfPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e44730 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lixin Zhang Jingping Xie Mayuri Patel Arsala Bakhtyar Garth D Ehrlich Azad Ahmed Josh Earl Carl F Marrs Daniel Clemans Timothy F Murphy Janet R Gilsdorf Nontypeable Haemophilus influenzae genetic islands associated with chronic pulmonary infection.
description	<h4>Background</h4>Haemophilus influenzae (Hi) colonizes the human respiratory tract and is an important pathogen associated with chronic obstructive pulmonary disease (COPD). Bacterial factors that interact with the human host may be important in the pathogenesis of COPD. These factors, however, have not been well defined. The overall goal of this study was to identify bacterial genetic elements with increased prevalence among H. influenzae strains isolated from patients with COPD compared to those isolated from the pharynges of healthy individuals.<h4>Methodology/principal findings</h4>Four nontypeable H. influenzae (NTHi) strains, two isolated from the airways of patients with COPD and two from a healthy individual, were subjected to whole genome sequencing using 454 FLX Titanium technology. COPD strain-specific genetic islands greater than 500 bp in size were identified by in silico subtraction. Open reading frames residing within these islands include known Hi virulence genes such as lic2b, hgbA, iga, hmw1 and hmw2, as well as genes encoding urease and other enzymes involving metabolic pathways. The distributions of seven selected genetic islands were assessed among a panel of 421 NTHi strains of both disease and commensal origins using a Library-on-a-Slide high throughput dot blot DNA hybridization procedure. Four of the seven islands screened, containing genes that encode a methyltransferase, a dehydrogenase, a urease synthesis enzyme, and a set of unknown short ORFs, respectively, were more prevalent in COPD strains than in colonizing strains with prevalence ratios ranging from 1.21 to 2.85 (p ≤ 0.0002). Surprisingly, none of these sequences show increased prevalence among NTHi isolated from the airways of patients with cystic fibrosis.<h4>Conclusions/significance</h4>Our data suggest that specific bacterial genes, many involved in metabolic functions, are associated with the ability of NTHi strains to survive in the lower airways of patients with COPD.
format	article
author	Lixin Zhang Jingping Xie Mayuri Patel Arsala Bakhtyar Garth D Ehrlich Azad Ahmed Josh Earl Carl F Marrs Daniel Clemans Timothy F Murphy Janet R Gilsdorf
author_facet	Lixin Zhang Jingping Xie Mayuri Patel Arsala Bakhtyar Garth D Ehrlich Azad Ahmed Josh Earl Carl F Marrs Daniel Clemans Timothy F Murphy Janet R Gilsdorf
author_sort	Lixin Zhang
title	Nontypeable Haemophilus influenzae genetic islands associated with chronic pulmonary infection.
title_short	Nontypeable Haemophilus influenzae genetic islands associated with chronic pulmonary infection.
title_full	Nontypeable Haemophilus influenzae genetic islands associated with chronic pulmonary infection.
title_fullStr	Nontypeable Haemophilus influenzae genetic islands associated with chronic pulmonary infection.
title_full_unstemmed	Nontypeable Haemophilus influenzae genetic islands associated with chronic pulmonary infection.
title_sort	nontypeable haemophilus influenzae genetic islands associated with chronic pulmonary infection.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/86e36140375842389a45b4a3ba0d2ad5
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Nontypeable Haemophilus influenzae genetic islands associated with chronic pulmonary infection.

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