Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles

Skeletal muscle growth and maintenance depend on two tightly regulated processes, myogenesis and muscle regeneration. Both processes involve a series of crucial regulatory molecules including muscle-specific microRNAs, known as myomiRs. We recently showed that four myomiRs, miR-1, miR-133a, miR-133b...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chrystalla Mytidou, Andrie Koutsoulidou, Margarita Zachariou, Marianna Prokopi, Konstantinos Kapnisis, George M. Spyrou, Andreas Anayiotos, Leonidas A. Phylactou
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	skeletal muscle muscle endogenous muscle-derived exosomes age myomiRs differential expression Physiology QP1-981
Acceso en línea:	https://doaj.org/article/86f3128605ab452590a0feba08a9e1c5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:86f3128605ab452590a0feba08a9e1c5
record_format	dspace
spelling	oai:doaj.org-article:86f3128605ab452590a0feba08a9e1c52021-11-18T09:20:07ZAge-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles1664-042X10.3389/fphys.2021.708278https://doaj.org/article/86f3128605ab452590a0feba08a9e1c52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.708278/fullhttps://doaj.org/toc/1664-042XSkeletal muscle growth and maintenance depend on two tightly regulated processes, myogenesis and muscle regeneration. Both processes involve a series of crucial regulatory molecules including muscle-specific microRNAs, known as myomiRs. We recently showed that four myomiRs, miR-1, miR-133a, miR-133b, and miR-206, are encapsulated within muscle-derived exosomes and participate in local skeletal muscle communication. Although these four myomiRs have been extensively studied for their function in muscles, no information exists regarding their endogenous and exosomal levels across age. Here we aimed to identify any age-related changes in the endogenous and muscle-derived exosomal myomiR levels during acute skeletal muscle growth. The four endogenous and muscle-derived myomiRs were investigated in five skeletal muscles (extensor digitorum longus, soleus, tibialis anterior, gastrocnemius, and quadriceps) of 2-week–1-year-old wild-type male mice. The expression of miR-1, miR-133a, and miR-133b was found to increase rapidly until adolescence in all skeletal muscles, whereas during adulthood it remained relatively stable. By contrast, endogenous miR-206 levels were observed to decrease with age in all muscles, except for soleus. Differential expression of the four myomiRs is also inversely reflected on the production of two protein targets; serum response factor and connexin 43. Muscle-derived exosomal miR-1, miR-133a, and miR-133b levels were found to increase until the early adolescence, before reaching a plateau phase. Soleus was found to be the only skeletal muscle to release exosomes enriched in miR-206. In this study, we showed for the first time an in-depth longitudinal analysis of the endogenous and exosomal levels of the four myomiRs during skeletal muscle development. We showed that the endogenous expression and extracellular secretion of the four myomiRs are associated to the function and size of skeletal muscles as the mice age. Overall, our findings provide new insights for the myomiRs’ significant role in the first year of life in mice.Chrystalla MytidouChrystalla MytidouAndrie KoutsoulidouAndrie KoutsoulidouMargarita ZachariouMargarita ZachariouMarianna ProkopiMarianna ProkopiKonstantinos KapnisisGeorge M. SpyrouGeorge M. SpyrouAndreas AnayiotosLeonidas A. PhylactouLeonidas A. PhylactouFrontiers Media S.A.articleskeletal musclemuscle endogenousmuscle-derived exosomesagemyomiRsdifferential expressionPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	skeletal muscle muscle endogenous muscle-derived exosomes age myomiRs differential expression Physiology QP1-981
spellingShingle	skeletal muscle muscle endogenous muscle-derived exosomes age myomiRs differential expression Physiology QP1-981 Chrystalla Mytidou Chrystalla Mytidou Andrie Koutsoulidou Andrie Koutsoulidou Margarita Zachariou Margarita Zachariou Marianna Prokopi Marianna Prokopi Konstantinos Kapnisis George M. Spyrou George M. Spyrou Andreas Anayiotos Leonidas A. Phylactou Leonidas A. Phylactou Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles
description	Skeletal muscle growth and maintenance depend on two tightly regulated processes, myogenesis and muscle regeneration. Both processes involve a series of crucial regulatory molecules including muscle-specific microRNAs, known as myomiRs. We recently showed that four myomiRs, miR-1, miR-133a, miR-133b, and miR-206, are encapsulated within muscle-derived exosomes and participate in local skeletal muscle communication. Although these four myomiRs have been extensively studied for their function in muscles, no information exists regarding their endogenous and exosomal levels across age. Here we aimed to identify any age-related changes in the endogenous and muscle-derived exosomal myomiR levels during acute skeletal muscle growth. The four endogenous and muscle-derived myomiRs were investigated in five skeletal muscles (extensor digitorum longus, soleus, tibialis anterior, gastrocnemius, and quadriceps) of 2-week–1-year-old wild-type male mice. The expression of miR-1, miR-133a, and miR-133b was found to increase rapidly until adolescence in all skeletal muscles, whereas during adulthood it remained relatively stable. By contrast, endogenous miR-206 levels were observed to decrease with age in all muscles, except for soleus. Differential expression of the four myomiRs is also inversely reflected on the production of two protein targets; serum response factor and connexin 43. Muscle-derived exosomal miR-1, miR-133a, and miR-133b levels were found to increase until the early adolescence, before reaching a plateau phase. Soleus was found to be the only skeletal muscle to release exosomes enriched in miR-206. In this study, we showed for the first time an in-depth longitudinal analysis of the endogenous and exosomal levels of the four myomiRs during skeletal muscle development. We showed that the endogenous expression and extracellular secretion of the four myomiRs are associated to the function and size of skeletal muscles as the mice age. Overall, our findings provide new insights for the myomiRs’ significant role in the first year of life in mice.
format	article
author	Chrystalla Mytidou Chrystalla Mytidou Andrie Koutsoulidou Andrie Koutsoulidou Margarita Zachariou Margarita Zachariou Marianna Prokopi Marianna Prokopi Konstantinos Kapnisis George M. Spyrou George M. Spyrou Andreas Anayiotos Leonidas A. Phylactou Leonidas A. Phylactou
author_facet	Chrystalla Mytidou Chrystalla Mytidou Andrie Koutsoulidou Andrie Koutsoulidou Margarita Zachariou Margarita Zachariou Marianna Prokopi Marianna Prokopi Konstantinos Kapnisis George M. Spyrou George M. Spyrou Andreas Anayiotos Leonidas A. Phylactou Leonidas A. Phylactou
author_sort	Chrystalla Mytidou
title	Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles
title_short	Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles
title_full	Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles
title_fullStr	Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles
title_full_unstemmed	Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles
title_sort	age-related exosomal and endogenous expression patterns of mir-1, mir-133a, mir-133b, and mir-206 in skeletal muscles
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/86f3128605ab452590a0feba08a9e1c5
work_keys_str_mv	AT chrystallamytidou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT chrystallamytidou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT andriekoutsoulidou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT andriekoutsoulidou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT margaritazachariou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT margaritazachariou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT mariannaprokopi agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT mariannaprokopi agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT konstantinoskapnisis agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT georgemspyrou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT georgemspyrou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT andreasanayiotos agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT leonidasaphylactou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles AT leonidasaphylactou agerelatedexosomalandendogenousexpressionpatternsofmir1mir133amir133bandmir206inskeletalmuscles
_version_	1718420912518201344

Age-Related Exosomal and Endogenous Expression Patterns of miR-1, miR-133a, miR-133b, and miR-206 in Skeletal Muscles

Ejemplares similares