Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Igor M Pongrac,1 Ivan Pavičić,2 Mirta Milić,2 Lada Brkič Ahmed,1 Michal Babič,3 Daniel Horák,3 Ivana Vinković Vrček,2 Srećko Gajović1 1School of Medicine, Croatian Institute for Brain Research, University of Zagreb, 2Institute for Medical Research and Occupational Health, Zagreb, Croatia...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pongrac IM, Pavičić I, Milić M, Brkić Ahmed L, Babič M, Horák D, Vinković Vrček I, Gajović S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	superparamagnetic iron oxide nanoparticles biocompatibility oxidative stress genotoxicity murine neural stem cells Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/86ff04ff7c914adfb11c022eb1de2e1b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:86ff04ff7c914adfb11c022eb1de2e1b
record_format	dspace
spelling	oai:doaj.org-article:86ff04ff7c914adfb11c022eb1de2e1b2021-12-02T00:30:22ZOxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles1178-2013https://doaj.org/article/86ff04ff7c914adfb11c022eb1de2e1b2016-04-01T00:00:00Zhttps://www.dovepress.com/oxidative-stress-response-in-neural-stem-cells-exposed-to-different-su-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Igor M Pongrac,1 Ivan Pavičić,2 Mirta Milić,2 Lada Brkič Ahmed,1 Michal Babič,3 Daniel Horák,3 Ivana Vinković Vrček,2 Srećko Gajović1 1School of Medicine, Croatian Institute for Brain Research, University of Zagreb, 2Institute for Medical Research and Occupational Health, Zagreb, Croatia; 3Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic Abstract: Biocompatibility, safety, and risk assessments of superparamagnetic iron oxide nanoparticles (SPIONs) are of the highest priority in researching their application in biomedicine. One improvement in the biological properties of SPIONs may be achieved by different functionalization and surface modifications. This study aims to investigate how a different surface functionalization of SPIONs – uncoated, coated with D-mannose, or coated with poly-L-lysine – affects biocompatibility. We sought to investigate murine neural stem cells (NSCs) as important model system for regenerative medicine. To reveal the possible mechanism of toxicity of SPIONs on NSCs, levels of reactive oxygen species, intracellular glutathione, mitochondrial membrane potential, cell-membrane potential, DNA damage, and activities of SOD and GPx were examined. Even in cases where reactive oxygen species levels were significantly lowered in NSCs exposed to SPIONs, we found depleted intracellular glutathione levels, altered activities of SOD and GPx, hyperpolarization of the mitochondrial membrane, dissipated cell-membrane potential, and increased DNA damage, irrespective of the surface coating applied for SPION stabilization. Although surface coating should prevent the toxic effects of SPIONs, our results showed that all of the tested SPION types affected the NSCs similarly, indicating that mitochondrial homeostasis is their major cellular target. Despite the claimed biomedical benefits of SPIONs, the refined determination of their effects on various cellular functions presented in this work highlights the need for further safety evaluations. This investigation helps to fill the knowledge gaps on the criteria that should be considered in evaluating the biocompatibility and safety of novel nanoparticles. Keywords: superparamagnetic iron oxide nanoparticles, biocompatibility, oxidative stress, genotoxicity, murine neural stem cellsPongrac IMPavičić IMilić MBrkić Ahmed LBabič MHorák DVinković Vrček IGajović SDove Medical Pressarticlesuperparamagnetic iron oxide nanoparticlesbiocompatibilityoxidative stressgenotoxicitymurine neural stem cellsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1701-1715 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	superparamagnetic iron oxide nanoparticles biocompatibility oxidative stress genotoxicity murine neural stem cells Medicine (General) R5-920
spellingShingle	superparamagnetic iron oxide nanoparticles biocompatibility oxidative stress genotoxicity murine neural stem cells Medicine (General) R5-920 Pongrac IM Pavičić I Milić M Brkić Ahmed L Babič M Horák D Vinković Vrček I Gajović S Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles
description	Igor M Pongrac,1 Ivan Pavičić,2 Mirta Milić,2 Lada Brkič Ahmed,1 Michal Babič,3 Daniel Horák,3 Ivana Vinković Vrček,2 Srećko Gajović1 1School of Medicine, Croatian Institute for Brain Research, University of Zagreb, 2Institute for Medical Research and Occupational Health, Zagreb, Croatia; 3Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic Abstract: Biocompatibility, safety, and risk assessments of superparamagnetic iron oxide nanoparticles (SPIONs) are of the highest priority in researching their application in biomedicine. One improvement in the biological properties of SPIONs may be achieved by different functionalization and surface modifications. This study aims to investigate how a different surface functionalization of SPIONs – uncoated, coated with D-mannose, or coated with poly-L-lysine – affects biocompatibility. We sought to investigate murine neural stem cells (NSCs) as important model system for regenerative medicine. To reveal the possible mechanism of toxicity of SPIONs on NSCs, levels of reactive oxygen species, intracellular glutathione, mitochondrial membrane potential, cell-membrane potential, DNA damage, and activities of SOD and GPx were examined. Even in cases where reactive oxygen species levels were significantly lowered in NSCs exposed to SPIONs, we found depleted intracellular glutathione levels, altered activities of SOD and GPx, hyperpolarization of the mitochondrial membrane, dissipated cell-membrane potential, and increased DNA damage, irrespective of the surface coating applied for SPION stabilization. Although surface coating should prevent the toxic effects of SPIONs, our results showed that all of the tested SPION types affected the NSCs similarly, indicating that mitochondrial homeostasis is their major cellular target. Despite the claimed biomedical benefits of SPIONs, the refined determination of their effects on various cellular functions presented in this work highlights the need for further safety evaluations. This investigation helps to fill the knowledge gaps on the criteria that should be considered in evaluating the biocompatibility and safety of novel nanoparticles. Keywords: superparamagnetic iron oxide nanoparticles, biocompatibility, oxidative stress, genotoxicity, murine neural stem cells
format	article
author	Pongrac IM Pavičić I Milić M Brkić Ahmed L Babič M Horák D Vinković Vrček I Gajović S
author_facet	Pongrac IM Pavičić I Milić M Brkić Ahmed L Babič M Horák D Vinković Vrček I Gajović S
author_sort	Pongrac IM
title	Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles
title_short	Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles
title_full	Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles
title_fullStr	Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles
title_full_unstemmed	Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles
title_sort	oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/86ff04ff7c914adfb11c022eb1de2e1b
work_keys_str_mv	AT pongracim oxidativestressresponseinneuralstemcellsexposedtodifferentsuperparamagneticironoxidenanoparticles AT pavicici oxidativestressresponseinneuralstemcellsexposedtodifferentsuperparamagneticironoxidenanoparticles AT milicm oxidativestressresponseinneuralstemcellsexposedtodifferentsuperparamagneticironoxidenanoparticles AT brkicahmedl oxidativestressresponseinneuralstemcellsexposedtodifferentsuperparamagneticironoxidenanoparticles AT babicm oxidativestressresponseinneuralstemcellsexposedtodifferentsuperparamagneticironoxidenanoparticles AT horakd oxidativestressresponseinneuralstemcellsexposedtodifferentsuperparamagneticironoxidenanoparticles AT vinkovicvrceki oxidativestressresponseinneuralstemcellsexposedtodifferentsuperparamagneticironoxidenanoparticles AT gajovics oxidativestressresponseinneuralstemcellsexposedtodifferentsuperparamagneticironoxidenanoparticles
_version_	1718403687029669888

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles

Ejemplares similares