BIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS

ABSTRACT Background: Bioactive compounds from the fruit of S. elaeagnifolium were isolated since could be highly potential source to develop functional foods or pharmaceutical products. Objectives: In this study a bioguided fractionation of the methanolic extract from S. elaeagnifolium fruit was...

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Autores principales:	Leobardo HERNÁNDEZ O, Pilar CARRANZA R, Luis Enrique COBOS P, Lluvia Itzel LÓPEZ L, Juan Alberto ASCASIO V, Sonia Yesenia SILVA B
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia
Materias:	Solanum fractionation bioguided cell line tumor Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/870809c22a11451db4774f0061522e6b
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spelling	oai:doaj.org-article:870809c22a11451db4774f0061522e6b2021-12-02T09:23:47ZBIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS0121-400410.17533/udea.vitae.v24n2a05https://doaj.org/article/870809c22a11451db4774f0061522e6bhttp://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-40042017000200124&lng=en&tlng=enhttps://doaj.org/toc/0121-4004ABSTRACT Background: Bioactive compounds from the fruit of S. elaeagnifolium were isolated since could be highly potential source to develop functional foods or pharmaceutical products. Objectives: In this study a bioguided fractionation of the methanolic extract from S. elaeagnifolium fruit was carried out to evaluate their cytotoxicity and antitumoral potential on several cell lines and breast tumor explants, respectively. Methods: Microdilution method with A. salina was used to isolate bioactive compounds. Fractionation was performed by vacuum liquid chromatography, and the monitoring from fractions was done by thin layer chromatography. The effect of the fractions on viability in Vero, HeLa, and MCF-7 cells was assessed using WST-1 assay, whereas in breast tumor explants was evaluated by Alamar blue assay. A qualitative phytochemical analysis was performed to partially identify the compounds contained in the fractions and a spectroscopic characterization by RP-HPLC-MS was done to identify the group of compounds responsible for the effect on the cell lines and the mammary explants. Results: Several fractions were isolated from the fruit of S. elaeagnifolim. Notwithstanding, the FVLC7 showed a higher activity in A. salina assay. This fraction reduced the viability at 39 ± 1.67, 15.05 ± 0.09 and 66.10 ± 4 % in Vero, HeLa and MCF-7 cells, at 100 mg/mL, respectively. On the other hand, showed an effect in breast tumor explants obtained from a patient in remission. Qualitative phytochemical analysis showed that FVLC7 contains alkaloids, coumarins, and sesquiterpene lactones. Characterization by RP-HPLC-MS detected quinic acid, chlorogenic acid, dicaffeoylquinic acid as well as presence of an alkaloid. Conclusion: On this basis, our results suggest that cytotoxic effect of FVLC7 isolated from the fruit of S. elaeagnifolium could be mediated by quinic, chlorogenic, and dicaffeoylquinic acids.Leobardo HERNÁNDEZ OPilar CARRANZA RLuis Enrique COBOS PLluvia Itzel LÓPEZ LJuan Alberto ASCASIO VSonia Yesenia SILVA BUniversidad de AntioquiaarticleSolanumfractionationbioguidedcell linetumorFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 24, Iss 2, Pp 124-131
institution	DOAJ
collection	DOAJ
language	EN
topic	Solanum fractionation bioguided cell line tumor Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Solanum fractionation bioguided cell line tumor Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Leobardo HERNÁNDEZ O Pilar CARRANZA R Luis Enrique COBOS P Lluvia Itzel LÓPEZ L Juan Alberto ASCASIO V Sonia Yesenia SILVA B BIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS
description	ABSTRACT Background: Bioactive compounds from the fruit of S. elaeagnifolium were isolated since could be highly potential source to develop functional foods or pharmaceutical products. Objectives: In this study a bioguided fractionation of the methanolic extract from S. elaeagnifolium fruit was carried out to evaluate their cytotoxicity and antitumoral potential on several cell lines and breast tumor explants, respectively. Methods: Microdilution method with A. salina was used to isolate bioactive compounds. Fractionation was performed by vacuum liquid chromatography, and the monitoring from fractions was done by thin layer chromatography. The effect of the fractions on viability in Vero, HeLa, and MCF-7 cells was assessed using WST-1 assay, whereas in breast tumor explants was evaluated by Alamar blue assay. A qualitative phytochemical analysis was performed to partially identify the compounds contained in the fractions and a spectroscopic characterization by RP-HPLC-MS was done to identify the group of compounds responsible for the effect on the cell lines and the mammary explants. Results: Several fractions were isolated from the fruit of S. elaeagnifolim. Notwithstanding, the FVLC7 showed a higher activity in A. salina assay. This fraction reduced the viability at 39 ± 1.67, 15.05 ± 0.09 and 66.10 ± 4 % in Vero, HeLa and MCF-7 cells, at 100 mg/mL, respectively. On the other hand, showed an effect in breast tumor explants obtained from a patient in remission. Qualitative phytochemical analysis showed that FVLC7 contains alkaloids, coumarins, and sesquiterpene lactones. Characterization by RP-HPLC-MS detected quinic acid, chlorogenic acid, dicaffeoylquinic acid as well as presence of an alkaloid. Conclusion: On this basis, our results suggest that cytotoxic effect of FVLC7 isolated from the fruit of S. elaeagnifolium could be mediated by quinic, chlorogenic, and dicaffeoylquinic acids.
format	article
author	Leobardo HERNÁNDEZ O Pilar CARRANZA R Luis Enrique COBOS P Lluvia Itzel LÓPEZ L Juan Alberto ASCASIO V Sonia Yesenia SILVA B
author_facet	Leobardo HERNÁNDEZ O Pilar CARRANZA R Luis Enrique COBOS P Lluvia Itzel LÓPEZ L Juan Alberto ASCASIO V Sonia Yesenia SILVA B
author_sort	Leobardo HERNÁNDEZ O
title	BIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS
title_short	BIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS
title_full	BIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS
title_fullStr	BIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS
title_full_unstemmed	BIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS
title_sort	bioguided fractionation from solanum elaeagnifolium to evaluate toxicity on cellular lines and breast tumor explants
publisher	Universidad de Antioquia
url	https://doaj.org/article/870809c22a11451db4774f0061522e6b
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BIOGUIDED FRACTIONATION FROM Solanum elaeagnifolium TO EVALUATE TOXICITY ON CELLULAR LINES AND BREAST TUMOR EXPLANTS

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